... had unsubstituted hydroxyl group at C-1, and was
mostly present in an a-pyranose form. Acyl
1
,acyl
2
and acyl
3
residues had hydroxy or acyloxy groups at C-3 (
13
C signals
of C-3 at 69.0–72.4 ... Structural analysis of
Francisella tularensis
lipopolysaccharide
Evgeny Vinogradov, Malcolm B. Perry and J. Wayne Conlan
Institute for Biological Sciences, ... 2-N-(3-O-
acyl
4
-acyl...
... chemical
analysis of 7-O-(2-amino-2-deoxy-a-
D
-glucopyranosyl-
L
-glycero-
D
-manno-heptose as a constituent of the lipopolysaccharides of the
UDP-galactose epimerase less mutant J-5 of Escherichia ... structure of E. coli R3 and R4 LPS (Eur. J. Biochem. 269) 5991
Structural analysis of deacylated lipopolysaccharide
of
Escherichia coli
strains 2513 (R4 core-type)
and F6...
... amounts of sialylated Hex3 glycoforms were indicated.
b
Minor amounts of Hex2 and Hex3 glycoforms lacking acetyl groups or
containing a second glycyl group were indicated.
c
Very minor amounts of ... Computer-
assisted structural analysis of polysaccharides with an extended
version of CASPER using
1
H- and
13
C-N.M.R. d ata. Carbohydr.
Res. 188, 169–191.
Ó FEBS 2002 Structural...
... biology and biochemistry have enabled analy-
sis of the expression profiles of numerous proteins at
once.
In this study, we looked at hypoxia-induced
apoptosis and, by using the Western array ... apoptosis-related proteins induced
by hypoxia in trophoblastic cells so as to clarify the mechanism of hypoxia-induced apoptosis by using the
PoweBlot, an antibody-based Western array. Hum...
... of hCDC4 can also be observed in
Acute Myeloid Leukemia (AML) or high risk
Myelodysplastic Syndrome (MDS). Therefore we
carried out a mutational analysis of the hCDC4 gene in
35 samples of ... Big Dye Terminator
system (Applied Biosystems, Germany). Sequences
were analyzed using the Chromas software (Technely-
sium Pty Ltd, Tewantin, Australia) and GeneDoc
software (http://www....
...
levels of many of these genes identified in the array
analysis. Moreover, Western blot analysis of thymo-
cyte lysates also confirmed that IgM increases with
age (Figure 3). Flow cytometry analysis ... and array analysis. For each array
sample, the RNA was prepared from the purified
thymocytes. The thymocytes were processed by using
the RNEasy Mini Kit (Qiagen, Valencia, CA)....
... capacity of DM64 to inhibit the myotoxicity induced
by myotoxins I (Asp49) and II (Lys49) from B. asper venom
was analyzed in vivo and in vitro. DM64 effectively neut-
ralized the myotoxic and cytotoxic ... completely
inhibited the toxins’ enzymatic activity, although residual
myotoxicity was still observed. Furthermore, the existence
of Lys49 PLA
2
structural variants displaying myotox...
... chaperoning in vitro [66], and disassembly of active units
from an oligomeric (storage) state of a-crystallin was
proposed, upon structural analysis of aA-crystallin by site-
directed spin labelling, ... example of stress resistance, fully hydrated cysts
survive several years at physiological temperature in the
complete absence o f o xygen [48,51,52,54], an unusual degree
of tol...
... sites of chymotrypsin. D-Chymotrypsinogen is a
chimera constructed to contain a trypsinogen propeptide
instead of the Cys1–Cys122-linked wild-type chymotryp-
sinogen peptide. D-Chymotrypsinogen ... of wild-type chymotrypsino-
gen, a variant of rat chymotrypsinogen (denoted as
D-chymotrypsinogen) was used throughout this study. The
Correspondence to L. Gra
´
f, Department of Biochemi...
... formation of gamma glutamyl-lysine bonds [4].
Furthermore, tTG plays a role in the transduction of
extracellular signals, mediated by its additional GTP-
hydrolyzing activity [5], analogous to that of ... vivo
inhibition of the enzymatic activity of tTG by CD
autoantibodies should be carefully considered. CD is
frequently accompanied by other autoimmune pathologies
whose onset might...