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Báo cáo Y học: Solution structure of the Alzheimer amyloid b-peptide (1–42) in an apolar microenvironment Similarity with a virus fusion domain potx

Báo cáo Y học: Solution structure of the Alzheimer amyloid b-peptide (1–42) in an apolar microenvironment Similarity with a virus fusion domain potx

Báo cáo Y học: Solution structure of the Alzheimer amyloid b-peptide (1–42) in an apolar microenvironment Similarity with a virus fusion domain potx

... purity assessed by a MALDI-TOF analysis using a Hewlett Packard G202 5A LD-TOF system mass spectro-meter and a- cyano-4-hydroxycinnamic acid as matrix.Sample preparationIt has been shown that a ... [38].Accordingly, it is possible that the mechanism of membraneinteraction and destabilization is different in the case of Ab,but it is fair to say that the similarity with the fusion domain of ... stronglyhydrophobic side chains; this feature has been aptlydescribed by Rajan et al. as a Teflon coating that cansurround a helix [16] in the case of a mixture of water andhexafluoroacetone hydrate,...
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Tài liệu Báo cáo Y học: Solution structure of the mEGF/TGFa44250 chimeric growth factor doc

Tài liệu Báo cáo Y học: Solution structure of the mEGF/TGFa44250 chimeric growth factor doc

... molecule and because of inaccuracies in the starting structure. Although mathemat-ical convergence may be achieved by iterative calculations, the accuracy of the distances obtained remains uncertain.Therefore, ... 2001geometry, the set of solutions reflects any uncertainty in the calibration as well as alternative fits to the set of distances. The main advantages of the procedure are simple: the details of the calibration ... exponential of the matrix of theoretical cross relaxation rates. These valuesmay be replaced by scaled experimental values and the logarithm of the matrix may be taken to obtain distances thattake account...
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Báo cáo Y học: Solution structure of the HIV gp120 C5 Domain pptx

Báo cáo Y học: Solution structure of the HIV gp120 C5 Domain pptx

... carboxy termini in close proximity. As shownby Fig. 5A, the amino terminal end of the C5 domain would be attached to remaining domains of gp120 in the native structure. The carboxy terminal end of ... and target cell membranes isfacilitated by the structures of gp41 and CD4 receptor.Specifically, gp41 contains a transmembrane domain thatis C-terminal to the ectodomain and thus located at the ÔleftÕ ... directing viralparticles to the appropriate target cell and initiating the fusion of the viral and cellular membranes in diverse virusesincluding retroviridae, herpesviridae, filoviridaie and para-myxoviridae...
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Báo cáo Y học: Functional studies of the Synechocystis phycobilisomes organization by high performance liquid chromatography on line with a mass spectrometer docx

Báo cáo Y học: Functional studies of the Synechocystis phycobilisomes organization by high performance liquid chromatography on line with a mass spectrometer docx

... chromatogram abundant in hydrophilic proteinssuch as linkers and a significant amount of a- andb-phycocyan ins, and only traces of the allophycocyanins.On the contrary, HPLC a nalysis of bands ... allophycoc yaninholds the bridging pigments between phycobilisomes and the photosynthetic lamellae [6]. Both phycocyanin andallophycocyanin are composed of two polypeptide chains, a and b, of approximately ... phycobilisomes beforeand after illumination reve aled the d isappearance of the b-phycocyanin already after 1 h of illumination (Fig. 4). In contrast, SDS/PAGE analysis of the total mixture of phycobilisomes...
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Báo cáo Y học: Solution structure of a hydrophobic analogue of the winter flounder antifreeze protein doc

Báo cáo Y học: Solution structure of a hydrophobic analogue of the winter flounder antifreeze protein doc

... changes wereTable 1. Sequence alignment of TTTT and VVVV2KE.1 2 13 24 35TTTT D TASDAAAAAAL TAANAKAAAEL TAANAAAAAAA TARVVVV2KE D VASDAKAAAEL VAANAKAAAEL VAANAKAAAEA VARCONH21260 E. Liepinsh ... calculations, and a short distance restraint was artificially introduced betweenAla14 H a and Lys19 HN, at the expense of an increasednumber of distance-restraint violations in the resultingconformers. ... prevented the resolution of allcross peaks. In particular, the chemical shifts of residuesGlu11 and Ala14 were practically indistinguishable fromthose of Glu22 and Ala25 (Table 2). Yet, the appearance...
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Tài liệu Báo cáo khoa học: Solution structure of the matrix attachment region-binding domain of chicken MeCP2 ppt

Tài liệu Báo cáo khoa học: Solution structure of the matrix attachment region-binding domain of chicken MeCP2 ppt

... secondary -structure elements are well defined, as also indicated by the rmsdvalues given in Table 1. Analysis of the Ramachandran plotshows that the dihedral angles / and w for the secondary structure ... (the methyl-CpG-binding domains of rat MeCP2 and methyl-CpG-binding domain protein 1, respectively), exhibiting a three-stranded antiparallel b-sheet and an a- helix a 1.We show that the C-terminal portion of ... contains several arginine and lysine side chainsforming hydrogen bonds with the bases and contacting the DNA backbone. The solution structure of the MBD of MBD1 shows high similarity to that of MeCP2...
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Báo cáo khoa học: Solution structure of the catalytic domain of RICH protein from goldfish pot

Báo cáo khoa học: Solution structure of the catalytic domain of RICH protein from goldfish pot

... polypeptide chain with three domains.Despite the low sequence similarity, the domain organization of plant and fungal ligases is very sim-ilar: an N-terminal adenylyltransferase ⁄ ligase domain, followed ... elements and the N- and C-termini are labeled. (C) Backbone overlay of catalytic domains of goldfish RICH (in cyan) and human CNPase (in purple) showing overall similarity of the structures. The lowest-energy ... phosphodiesterase domainsare shown in purple. RICH and CNPasecontain a C-terminal isoprenylation motifshown in red, domains with experimentallyconfirmed polynucleotide kinase and aden-ylyltransferase activity...
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Báo cáo khoa học: Solution structure of the active-centre mutant I14A of the histidinecontaining phosphocarrier protein from Staphylococcus carnosus ppt

Báo cáo khoa học: Solution structure of the active-centre mutant I14A of the histidinecontaining phosphocarrier protein from Staphylococcus carnosus ppt

... wild-typeprotein in the standard complementation a ssay [48].Reversal of the mutation-induced changesby xenon-bindingOne might reasonably assume that the removal of the bulkysidechain of an isoleucine ... that a xenon atombinds into the hydrophobic cavity of HPr(I1 4A) that i screated by the replacement of t he bulky Ile14 by an alanine (Fig. 6). P otentially, the binding of xenon insidethis cavity ... must be able to interact with different proteinsand ligands in a tightly regulated manner mainly dependingupon the nutritional state of the bacterium. Probably thesedifferent interactions are...
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Báo cáo Y học: Crystal structure of the catalytic domain of a human thioredoxin-like protein pdf

Báo cáo Y học: Crystal structure of the catalytic domain of a human thioredoxin-like protein pdf

... negative) than the latter. As the C-terminal regionis rich in acidic amino acids, if it does have some interaction with the N-terminal domain, the mechanism of regulating the catalytic activity ... Zhou2, Mark Bartlam1, Qing Guo1, Yiwei Liu1, Yixin Sun1, Yu Gao1,2,Sheng Ye1, Guangtao Li2, Zihe Rao1, Boqin Qiang2and Jiangang Yuan21Laboratory of Structural Biology and the ... for a protein with 289 amino acids and a calculated molecular mass of 32 kDa. A search of the nonredundant protein sequencedatabase was performed using the BLASTprogram. Besidessharing the same...
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Báo cáo khoa học: Solution structure of the bb¢ domains of human protein disulfide isomerase docx

Báo cáo khoa học: Solution structure of the bb¢ domains of human protein disulfide isomerase docx

... analysis of PDI revealed thatloss of the two cysteines in the C-terminal a domain increased the Kmto 30 lm, and loss of an additional cys-teine in the a domain resulted in an increase of the ... is a twist of over 120° in the relativeorientations of the a and b domains. In one struc-ture, the catalytic cysteines face each other; in the other, the catalytic residues of the a domain faceaway ... titrations of 2 mM mastoparan by human PDI-bb¢protein and a plot of the magnitude of changes in mastoparanproton chemical shifts at a protein ⁄ mastoparan ratio of 1 : 15. A. Y. Denisov et al. Structure...
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