Báo cáo khoa học: Secretion of macrophage urokinase plasminogen activator is dependent on proteoglycans potx

Tài liệu Báo cáo khoa học: Expression of poly(A)-binding protein is upregulated during recovery from heat shock in HeLa cells doc

... linearresponse of the detection technique.This supplementary material can be found in theonline version of this article.Please note: Wiley-Blackwell is not responsible forthe content or functionality ... TOP-containing constructthan what was observed with the construct containingTOP alone. This is in good agreement with previousobservations that the ARS slows the rate of translationinitiation ... [22].DiscussionTranslational control of PABP1 expression duringrecovery from heat shockPABP1 is considered to be a genuine translation initia-tion factor [29], and its activity and expression...

Báo cáo khoa học: Acceleration of disulﬁde-coupled protein folding using glutathione derivatives potx

... (solvent-exposed disulfide bonds) and GSSGpromotes the formation of disulfide bonds in proteins,resulting in the formation of the native conforma-tion of a protein. Glutathione (c-GSH) contains ac-branched ... disulfide bonds,including mis-bridged disulfide bonds, two disulfidebonds, no disulfide bonds and native disulfide bonds,respectively, as confirmed by MALDI-TOF ⁄ MS analy-ses. The folding reactions ... reaction, is absent, although trace amounts of RCGredexist as a side reaction product of the forma-tion of the cross-disulfide bond. Therefore, the intra-molecular disulfide-exchange reaction...

Báo cáo khoa học: Control of mammalian gene expression by amino acids, especially glutamine potx

... effect of amino acids on gene expression.Concerning the stimulation of gene expression by thepresence of amino acids, only one gene, Pept 1, encod-ing a peptide transporter, was shown to contain ... representation of theinﬂuence of glutamine on the transcription of genes involved in intermediarymetabolism.Table 5. Inﬂuence of glutamine on transcription factors involved in cell proliferation, ... & Kato H(1998) Analysis of gene expression in homocysteine-injured vascular endothelial cells: demonstration of GRP78/BiP expression, cloning and characterization of a novel reducing agent-tunicamycin...

Báo cáo khoa học: Design of expression vectors for RNA interference based on miRNAs and RNA splicing potx

... compilation ª 2006 FEBSwhich consist of intronic miRNAs in protein-codingtranscripts [8,9]. Separation by splicing of the miRNAcomponent from the 5¢ CAP-exon-EGFP-3 ¢ poly(A)component should ... processing of the pri-miRNA is relatively efﬁcient even when the miRNAcassette is in an exon, but correspondingly that most of the marker protein expression is lost through degra-dation of the ... expression cassette is a successful generalstrategy for improving marker gene protein expression.In this study, insertion of the miRNA-basedartiﬁcial miRNA expression cassette into an intronABFig....

Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... years ago [4]. On the basis of this long period of co-evolution, there are some indica-tions that colonization by H. pylori could have beenbeneﬁcial for their human carriers and this probablyprovided ... polarity, cell proliferation, actin-cytoskeletal rearrangements, cell elongation, disruption of tight and adherens junctions, pro-inﬂammatoryresponses and the suppression of apoptosis, as depicted. Black ... The contribution of these signallingpathways to pathogenesis during H. pylori infections is discussed.AbbreviationsAP, activator protein; cagPAI, cytotoxin-associated gene-pathogenicity island;...

Tài liệu Báo cáo khoa học: Impact of the native-state stability of human lysozyme variants on protein secretion by Pichia pastoris doc

... a reduction in the thermostability of their nativestates. Analysis of mRNA levels in this selection of variants suggests thatthe lower levels of secretion are due to post-transcriptional processes, ... and location of themutations discussed in this study. The locations of the single-pointmutations are shown on the structure of human wild-type lyso-zyme, deﬁned by X-ray diffraction (PDB entry ... compilation ª 2006 FEBS 713the less stable proteins is a result of degradation of partially folded or misfolded proteins by the qualitycontrol system during secretion. In light of the correla-tion...

Báo cáo khoa học: Architecture of the Helicobacter pylori Cag-type IV secretion system pdf

... reside only in the C-terminal por-tion (Fig. 3C). This discrepancy is particularly appar-ent for HP0527 that consists of almost 2000 residues, of which only 400 at the C-terminus correspond toVirB10 ... function of which is unknown, is oftenassociated with VirB4. In certain bacteria, some of these components are absent. This is the case for theH. pylori ComB system where the apparatus is used ... named CagE). On the basis of sequence signature, HP0544 containsmotif conserved in both VirB3 and VirB4 proteins[34]. This is reminiscent of the Campylobacter jejuniFig. 2. Structures of cytoplasmic...

Báo cáo khoa học: "Impact of computerized physician order entry on medication prescription errors in the intensive care unit: a controlled cross-sectional trial"

... wasresponsible for conceiving the study, data acquisition, analysis of the data, statistical analysis and drafting of the manuscript.BC was responsible for data acquisition, analysis of the ... severityclassification was modified, since this classification is ade-quate for administration errors, but not entirely for prescriptionerrors.An independent panel, consisting of one clinical pharmacist,not ... staff,consisting of five senior intensivists and three residents,rotated continuously over these units, usually on a one-weekbasis. One month after the completion of the study, the ICISwas...

Báo cáo khoa học: " Effect of the medical emergency team on long-term mortality following major surgery"

... patients displayed signs of pneumonitis and pneumonia upon arrival. Three patientspresented with severe sinusitis. Microbiology identified a variety of common but also uncommon isolates that were often ... Psychoemotional responses further com-prised distress about injuries sustained, dissociation, optical,acoustical and olfactory intrusions and, in some cases, agita-tion.DiscussionWe report on our ... has been discussed [49].The area of disaster mental health is fairly new and only fewdata exist on what interventions may encounter short and longterm psychological problems. One reason why valid...

Báo cáo khoa học: "Effect of intern’s consecutive work hours on safety, medical education and professionalism"

... thesafety of patient care. We believe, however, that development of ‘a sense of professionalism’ is not a function of whether ashift is 30 hours or is 16 hours, but is a function of the ethicalpriorities ... [2].LetterEffect of intern’s consecutive work hours on safety, medicaleducation and professionalismChristopher P Landrigan, Steven W Lockley and Charles A CzeislerDivision of Sleep Medicine, Boston, Massachusetts, ... of disciplines. We likewise agree that optimizing patient hand-offs, medical education, and trainees’ sense of professionalism should be priorities as interventions aredeveloped that reduce consecutive...

Xem thêm