Báo cáo khoa học: Proper targeting and activity of a nonfunctioning thyroid-stimulating hormone receptor (TSHr) combining an inactivating and activating TSHr mutation in one receptor pptx

... Proper targeting and activity of a nonfunctioning thyroid-stimulating hormone receptor (TSHr) combining an inactivating and activating TSHr mutation in one receptor Patrizia Agretti1, ... described as a cause of congenital hypothyroidism. Theeﬀects of combining activating and inactivating mutationswithin a single receptor was studied. The double mutantT477I/P639S contained an activating ... mutations within a single receptor. We decided tocombine two particularly potent activating TSHr mutations and to combine an activating mutation together with an inactivating one. Double mutant receptors...

Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by ﬂuorescence resonance energy transfer and the role of ATP hydrolysis pptx

... strand exchange assay weresynthesized by Metabion (Martinsreid, Germany) with thefollowing sequences: PhiC: 5¢-CGATACGCTCAAAGTCAAAATAATCAGCGTGACATTCAGAAGGGTAATAAGAACG-3¢;, PhiW: 5¢-CGTTCTTATTACCCTTCTGAATGTCACGCTGATTATTTTGACTTTGAGCGTATCG-3¢ and ... the sta-tus of physical union and separation of complementarystrands during renaturation and strand exchange,respectively, and thereby assesses the recombinase activity of OsDmc1, as has been ... stranded DNA binding activity. Binding tosingle stranded DNA stimulated a signiﬁcant level of DNA dependent ATPase activity. OsDmc1 alsoshowed renaturation of complementary strands as wellas...

Báo cáo khoa học: Anti-HIV-1 activity of 3-deaza-adenosine analogs Inhibition of S-adenosylhomocysteine hydrolase and nucleotide congeners pot

... uniqueantiviral activity of the DZA analogs. As AdoHcy hydrolaseinhibitors, DZA and DZAri have been shown to decreasethe AdoMet/AdoHcy ratio and inhibit methylation of DNA, RNA, protein, lipid, and ... West Palm Beach, Florida, USA;6BioinformaticsLaboratory, International Institute of Molecular and Cell Biology, Warsaw, PolandEight adenosine analogs, 3-deaza-adenosine (DZA), 3-deaza-(±)aristeromycin ... v/v/v).The radioactivity was quantitated by cutting the plasticbacked TLC plates and placing them in scintillation vials, and counting in a Packard 2000 CA scintillation counter(Packard Instruments,...

Tài liệu Báo cáo khoa học: Post-translational modiﬁcation of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion pptx

... serine ⁄ threonine kinase activity [13,15,37] and binding to actin [12], YpkA hasbeen shown to interact with small GTPases and inhibitnucleotide exchange in Rac1 and RhoA, mimicking theguanidine ... immunoprecipitation of OTUB1. As a control, lysate was incubated with agarose without the antibody. Immunoprecipitatedmaterial was analysed by SDS ⁄ PAGE and silver staining, and the large band corresponding ... separated by SDS ⁄ PAGE and visualized using silver staining, as described in Edelmann et al. [29]. Gel bands that were unique to lanescontaining OTUB1 as well as the corresponding areas in the...

Báo cáo khoa học: The potent inhibitory activity of histone H1.2 C-terminal fragments on furin doc

... column and RP-HPLC, and analyzed by SDS ⁄ PAGE and massspectrometry (Fig. 5). The molecular masses of therecombinant full-length histone H1.2 and its N- and C-terminal fragments determined by mass ... spectrometryanalysis of the recombinant full-length his-tone H1.2 and its N- and C-terminal frag-ments. (A) The recombinant full-lengthhistone H1.2 and its N- and C-terminal frag-ments were ... thepeptide inhibitor decanoyl-Arg-Val-Lys-Arg-CH2Cl and the bioengineered variant of a1 -antitrypsin Portland (a1 -PDX), the latter of which is highly selective for furin in vitro (Ki¼ 0.6 nm) and has...

Báo cáo khóa học: S-(2,3-Dichlorotriazinyl)glutathione A new afﬁnity label for probing the structure and function of glutathione transferases potx

... Zeta, Phi, Tau and O mega [3,4,9]. Whereas Zeta, Theta and Omega classes of GSTs are found in plants and animals, the large Phi and Tau classes a re unique to plants [9]. In maize ( Zea m ays L),42 ... the absence of anystructural perturbation caused by the mutation. Amino-acid analysis of the SDTG-modiﬁed Phe51Ala mutant and determination of its total amino and carboxy c ontentsuggests that ... chains are 26.67 A ˚2 and 49.26 A ˚2,andtheB-factors of S atoms are 38.14 A ˚2 and 79.30 A ˚2, respect-ively. It is therefore reasonable to propose that conform-ational changes and changes...

Báo cáo khoa học: Acute intermittent porphyria – impact of mutations found in the hydroxymethylbilane synthase gene on biochemical and enzymatic protein properties pdf

... Shoolingin-Jordan PM, Al-Dbass A, McNeill LA,Sarwar M & Butler D (2003) Human porphobilinogendeaminase mutations in the investigation of the mecha-nism of dipyrromethane cofactor assembly and ... as the standard and 12.5% TCA as a blank. The exactconcentration was determined at room temperature by mea-suring A 405 and calculated as A 405⁄ e (e – 505 · 103LÆcm)1Æmol)1). A standard ... consent was obtained from eachpatient, and the study was carried out in accordance with theprincipals of the Declaration of Helsinki.Isolation and ampliﬁcation of DNAGenomic DNA was extracted...

Báo cáo khoa học: Lpe10p modulates the activity of the Mrs2p-based yeast mitochondrial Mg2+channel pot

... ⁄ PAGE gels. HA protein-containing bandswere visualized by the use of an anti-HA serum (Covance), and myc protein-containing bands by an anti-Myc serum(Sigma-Aldrich). Chemical cross-linking ... was used with the mutagenicforward primer 5¢-GCTCTATTCCTGTCTATCGCTAGCTCTGTTCTGGAAAGTTTCATAGAAG-3¢ and the muta-genic reverse primer 5¢-CTTCTATGAAACTTTCCAGAACAG AGCTAGCGA TAGAACCCA GGAATAGAG ... that the mainte-nance of DW and that of Mg2+ in ux are functionally separated. Cross-linking and Blue native PAGE experiments indicated interaction of Lpe10pwith the Mrs2p-containing channel...

Báo cáo khoa học: Fibroblast growth-stimulating activity of S100A9 (MRP-14) ppt

... Chemistry, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University,Sugitani, Toyama, JapanFibroblasts play a critical role in chronic in ammation and wound healing. In this ... SDS/PAGE analyses of a ﬁbroblast growth-stimulating factor (FGSF) and recombinant S10 0A9 . FGSF puriﬁed by rechromatography of peak 1 indicated in Fig. 1, recombinant S10 0A9 and oxidized S10 0A9 (A9 ox) ... bacteria was harvested by centrifugation and lysed by freezing and thawing, sonication, and addition of 1% Triton X-100. The clear lysate was obtained bycentrifugation and applied to a Glutathione...

Báo cáo khoa học: Structured DNA promotes phosphorylation of p53 by DNA-dependent protein kinase at serine 9 and threonine 18 doc

... 13705; E-mail: ssoubeyrand@ohri.caAbbreviations: ATM, ataxia telangiectasia mutated gene product;ATR, ataxia telangectasia and RAD-3-related kinase; dsDNA,double-stranded DNA; DNA-PK, DNA-dependent ... roduct and t he ataxia t elangectasia and RAD-3-related kinase promote phosphorylation o f humanp53 at Ser15 and Ser20, and are required for the activation of p53 f ollowing DNA damage. DNA-dependent ... lM in all the samples and kinasing of p53wt(lanes 1, 2) or p53S1 5A/ S3 7A (lanes 3, 4) was performed by standardassay. On the left is a Phosphorimager analysis of a representative geland...

Xem thêm