Báo cáo khoa học: Functional symmetry in the isolated domain demonstrated by N-ethylmaleimide labelling pdf
Báo cáo khoa học: Functional symmetry in the isolated domain demonstrated by N-ethylmaleimide labelling pdf
... Biochem. 270) Ó FEBS 2003 The nucleotide-binding domains of P-glycoprotein Functional symmetry in the isolated domain demonstrated by N-ethylmaleimide labelling Georgina Berridge1, Jennifer ... chain to the nucleotide-binding pocket [3].We describe the engineering, expression and purification ofsingle-cysteine-containing proteins comprising either the C-terminal NBD or the N-terminal ... NBDs,suggesting that there is little asymmetry in the initial step of the transport cycle, namely the recognition and binding ofnucleotide. Any observed functional asymmetry in the intacttransporter...
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Báo cáo khoa học: Conserved residues in the N-domain of the AAA+ chaperone ClpA regulate substrate recognition and unfolding pdf
... twoelements in ClpA, one in the N -domain and the other in the pore of the ClpA hexamer. In the case of shortunstructured peptides or unfolded proteins such ascasein, binding to the tyrosine residues in ... forvarious adaptor proteins [6–10]. ClpA consists of threedomains: an N -domain and two ATP-binding domainsreferred to as the D1 and D2 domains. Interestingly,deletion of the N -domain from ClpA not ... debate regarding the role of the ClpBN -domain (which shares a common fold with the N -domain of ClpA) in substrate selection [15–17]. Onedifficulty in understanding the role of the N -domain ofClpA...
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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt
... DEC1(including the bHLH domain) is sufficient for the suppressive activity of DEC1. The C-terminal region(amino acids 140–412), including the Orange domain [18], i s not required for suppression in ... 4415whereas the N-terminal region, containing the bHLH domain, is not essential for the interaction.Dominant-negative DEC1 counteracts the suppressionof CLOCK/BMAL1-induced transcription in the presenceof ... suppressiveactivity against CLOCK/BMAL1-induced transcription. In addition, the N-terminal region of DEC1, including the bHLH domain, interacted with the C-terminal r egion o fBMAL1 in a mammalian two-hybrid...
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Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx
... removal of the N-terminal helices 1 and 2 while leaving the interactinga-helix 8 intact, may destabilize the proteins leading tospurious protein–protein or/and protein–DNA interactions. The crystal ... migration. The proposed DNA binding role of the triple-helix domain ofYdc2 is further supported by the observation in the NCBIdatabase that the N-terminal region of Ydc2 (residues 1–35)contains a ... correctpositioning of the SAP motifs in relation to the rest of the protein as well as for the stability of the protein. The truncated Ydc2 mutant proteins localizeto the mitochondria in S. pombeYdc2...
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Báo cáo khoa học: Factors involved in the assembly of a functional molybdopyranopterin center in recombinant Comamonas acidovorans xanthine dehydrogenase pot
... is shown in Fig. 9, where the structure around the MPT site in bovineXDH is shown and the corresponding amino acids in C. acidovorans are shown in parenthesis. The residuesaround the Mo site ... centers and anFAD into the recombinant enzyme. Therefore, the hostorganism has to be able to provide these cofactors and the means for their incorporation into the expressed protein in order to form ... to utilize cysteine as the sourceof sulfur [19]. Therefore, the level of sulfuration ofrecombinant XDH may be in uenced by the redox stateof the thiols in the cell. Indeed, the level of XDHABactivityobserved...
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Báo cáo khoa học: Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P. putida 86-1 Dqor pUF1 and analysis of the Qor proteins doc
... from quinoline [1,2]. Besides quino-line, some quinoline derivatives and the benzodiazinesquinazoline and quinoxaline are accepted as substrates [1,3].Like other enzymes catalysing the hydroxylation ... diminuta 7,coding for isoquinoline 1-oxidoreductase, were cloned in P. putida KT2440 and in the quinoline degrading strain,P. putida 86. However, the level of Ior synthesis was verylow in ... able to integrate the Mo-MCD cofactor into the corresponding apoprotein. In a first attempt to functionally express genes codingfor a Mo-MCD-containing hydroxylase in heterologoushosts, the iorAB...
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Báo cáo khoa học: Functional role of the linker region in purified human P-glycoprotein pot
... fractions containingP-gp, the results could be affected by other proteins. In fact, it was reported that the linker region of P-gpinteracts with other proteins such as RING fingerprotein 2 (RNF2) ... participate in neither the preservation of the overall structure nor the ATPase reaction itself. This issupported by the following findings. (i) Cleaving the linker did not inactivate the ATPase ... Fig. 7) with the N-terminal elbow helix of sub-unit B (shown in purple in Fig. 7) in the MsbA dimer. In the inward-facing state, there appears to be lessinteraction between the linker region...
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Tài liệu Báo cáo khoa học: Nuclear receptors in the mosquito Aedes aegypti Annotation, hormonal regulation and expression profiling ppt
... recovered,only the longest amino acid sequences that included the DNA binding, hinge and ligand-binding domainswere used for this analysis, except for the Knirps fam-ily members that lack the LBD. In D. ... vitellogenic femalemosquitoes. They isolated several clones that codefor a single protein. Interestingly, during the initialprocess of identifying the NR family members in the A. aegypti genome, we ... blood meal(PBM), peaking at 18–24 h PBM [3]. 20E is one of the primary regulators in the synthesis of vitellogenin (Vg), the main YPP protein produced by the fat body [4,5]. The molecular mechanism...
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Tài liệu Báo cáo khoa học: Electrostatic contacts in the activator protein-1 coiled coil enhance stability predominantly by decreasing the unfolding rate docx
... interpretation in distinguish-ing interacting from noninteracting proteins. OneFig. 1. Schematics of library designs. The helical wheel diagram looks down the axis from the N-terminus to the C-terminus. ... importance in our abil-ity to engineer increased protein–protein interactionstability at will; in particular, the ability to increasestability by kinetic design. For example, by achievingthis ... date [20].AP-1 foldingFurther insight into the structural determinants of sta-bility arose by dissecting the folding pathway of fourJun-based leucine zipper variants that bind with highaffinity...
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Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx
... 2; RIG-I, retinoic acid-inducible gene I; siRNA, small interfering RNA; TBK, Tank-binding kinase 1; TLR, Toll-like receptor; TRIF,Toll-IL1 receptor domain- containing adaptor inducing IFN-b.FEBS ... [8–10].Virus-infected cells trigger the innate immuneresponse by recognizing viral components, includingDNA, ssRNA, dsRNA and glycoproteins. Thisresponse initiates signaling pathways leading to the induction ... roles in the recognitionof the viral components [11,12].IFN-b is induced by dsRNA, a common intermedi-ate in many RNA virus infections, including HCV. The viral dsRNA as well as the synthetic...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpBáo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Sở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXChuong 2 nhận dạng rui roTăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ