Báo cáo khoa học: Purification of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays pdf
Báo cáo khoa học: Purification of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays pdf
... Purification of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays Greg B. G. Moorhead*, Sarah E. M. Meek, Pauline Douglas*, ... glutamine synthetase (GS) and nitrate reductase (NR) was highly purified from cauli-flower (Brassica oleracea var. botrytis) extracts. The finalpreparationcontainedanacyl-CoAoxidaseandasecond protein ... acyl-CoA oxidase and a second protein of the plant nucleotide pyrophosphatase family. Nucleotide pyrophosphatases belong to a family of widely distributed hydrolases that are active on a variety of derivatives...
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Tài liệu Báo cáo khoa học: Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx
... crosspeaks were assigned and inte-grated, with concomitant cycles of structure calcula-tions for evaluation of distance and angle constraintviolations as well as assignments of additional peaksbased ... China Sea. Sephadex G-25 was purchased fromAmersham Biosciences (Uppsala, Sweden), a ZORBAX300SB-C18 semipreparative column was from Agilent Tech-nologies (Santa Clara, CA, USA), and trifluoroacetic ... 275–283.10 Kuwada M, Teramoto T, Kumagaye KY, Nakajima K,Watanabe T, Kawai T, Kawakami Y, Niidome T,Sawada K, Nishizawa Y et al. (1994) Omega-agatoxin-TK containing D-serine at position 46, but...
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Tài liệu Báo cáo khoa học: Purification and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt
... ATCCTCACGAACAAGCAG5RACR GATCGCGATGCAGGCCTTFLF1 GGACGACTACAGCGTCTTCAGTAGAFLR1 TCCAAACAGTCAGTTTCTTAACCGTTable 1. Purification of cellulase from abalone Haliotis discus hannai. Oneunitofcellulasewasdefinedastheamountofenzymethatliberatesreducing ... et al. [29] using bovine serumalbumin fraction V as a standard protein. cDNA cloningConstruction of the cDNA library and cloning of cellulasecDNA was achieved as follows: Total RNA was extractedfrom ... cDNA was syn-thesized from the mRNA with a cDNA synthesis kit(TaKaRa, Tokyo, Japan) and used as an abalone cDNAlibrary. cDNAs encoding abalone cellulase were amplifiedby PCR from the cDNA library...
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Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
... sialidasetypeX,proteaseenzymes and molecular mass standards were purchasedfrom Sigma.Preparation of crab seraFreshwater field crabs, Paratelphusa jacquemontii werecollected from the local ... werewashed in NaCl/Tris five times and used for the hemagglu-tination assay.Preparation of asialo-erythrocytes. The procedure fol-lowed for preparing asialo-erythrocytes was that of Mercy and Ravindranath ... [34]. An evaluation of the literature revealed that purification of lectin from thehemolymph of crustaceans was most successful by affinitychromatography as it gave a higher fold of purification and percentage...
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Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf
... subunit of nitrate reductase from variousorganisms, such as NarI of nitrate reductase of Escherichiacoli [26]. AF501 also has low sequence similarity to theb-type cytochrome (HdrE) of Hdr. A topological ... polypeptide with anapparent molecular mass of 53 kDa appears as a double band inunboiled samples (lanes A1 and B1).Table 1. N-Terminal sequences of the polypeptides of the purified enzyme. N-Terminal ... iron, and acid-labile sulfur [36]. Anadditional protein with an apparent molecular mass of 11 k Da is associated with sulfite reductase from D. vulgaris[38] and De sulfovibrio desulfuricans [39,40]....
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Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... 21673TTREEQPAAEAAASTAGGSQACC ACC AGG GAA GAA CAA CCG GCG GCA GAG GCC GCG GCG TCC ACC GCT GGC GGT AGC CAA 27693QEGYGSTQLPSDEPLNGLNDCAA GAA GGA TAT GGC AGC ACC CAA CTT CCA TCG GAC GAG CCA TTG AAC GGG ... GGA GGC TAT GGC GGA GAG GGA TCA GCA GCC GCC CAG GAG GTC CAG ACC GCC GCC 15653QEAVEGAEQVASESASLTTPCAG GAG GCG GTA GAG GGC GCC GAG CAG GTA GCG TCC GAG TCA GCC TCC CTC ACA ACA CCA 21673TTREEQPAAEAAASTAGGSQACC ... 636213WKDTTSVGCGRVLCDDKKDTTGG AAG GAC ACC ACC AGC GTC GGA TGC GGA CGC GTC CTC TGC GAC GAC AAG AAG GAC ACC 696233MIMCSYWPPGNYENQKPYATG ATC ATG TGC AGC TAC TGG CCG CCG GGC AAC TAT GAA AAC CAG AAG CCC TAC 750BCyn...
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Báo cáo khoa học: Purification and properties of a new S-adenosyl-Lmethionine:flavonoid 4¢-O-methyltransferase from carnation (Dianthus caryophyllus L.) pot
... compo-nents of each reaction mixture, after their chromatographicseparation and purification, were submitted to1HNMR(nuclear magnetic resonance) and FABMS (fast atombombardment mass spectrum) analyses ... confirmed that it consistsactually of a unique enzymatic protein. Molecular mass and pI determination of F 4¢-OMT. Themolecular mass of the pure enzyme was calculated boththrough gel-filtration and ... Theconcentration of both the assayed compound and itsrespective transformation product was determined bycomparison of peak data with those obtained fromauthentic standards chromatographed at different...
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Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
... pGEX–EWS; EAD forward d(CGGAAT TCA TGG CGT CCA CGG ATT ACA G) and EADreverse d(CGC TCG AGT CAT CCG GAA AAT CCTCCA GAC T), for pGEX–EAD; RGG1 forward d(CGGAAT TCC CAG GAG AGA ACC GGA GCA T) and RGG1 ... compilation ª 2011 FEBSIdentification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein Kentaro Takahama1,*, Katsuhito Kino2,*, Shigeki Arai3, Riki Kurokawa3 and Takanori ... primers: KGG3-2 forwardd(AAA GGT GGC AAA GGT GGA GAC AGA GGTGGC TT) and KGG3-2 reverse d(GAA CAT TCC ACCGGG ACC ACC AC). pGEX–KGG3-4 was generated byPCR using pGEX–KGG2 as a template and the followingprimers:...
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Tài liệu Báo cáo khoa học: Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc
... AAG CCG ATG ACA CCA ATT (asd sense) This studyasd2 GCA GGT TCA TAG TGC ATG (asd antisense) This studyKan RP GGT GCG ACA ATC TAT CGA (kanamycin sense) [19]Kan FP CTC ATC GAG CAT CAA ATG (kanamycin ... mean value was calculated.Bactericidal assay with normal human serumComplement-sufficient normal human serum was prepared and pooled from eight healthy adult donors. A bactericidalassay was ... E. coliDNA polymerase I Klenow fragment, and Taq DNA poly-merase were purchased from Fermentas (Hanover, MD,USA). Preparation of plasmids, and purification of PCRproducts and DNA fragments,...
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Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf
... region wasdecreased and a new band was detected at 64 kDa, butonly after treatment with heparitinase II (Fig. 5A) .Although the same band was obtained after digestionFig. 3.45Ca overlay analysis ... found to have a molecular mass of 64 kDa, and to containtwo tandem repeats and a Glu-rich region. The structure of the protein and that of its DNA are similar to those of starmaker, a protein involvedin ... byinvertebrates, and has three crystal phases: calcite, ara-gonite and vaterite. Although calcite is the most stablecrystal thermodynamically, many organisms can formmetastable aragonite crystals with...
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Từ khóa: chuyên đề điện xoay chiều theo dạngđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Sở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt nam