... Assessment of porcine and human 16-ene-synthase, a third activity
of P450c17, in the formation of an androstenol precursor
Role of recombinant cytochrome
b
5
and P450 reductase
Penny ... showed
elution peaks at 4.70, 2.30 and 2.50 min, respectively
(Fig. 2). In both porcine and human assays using preg as
a substrate, an additional peak of...
... kDa, 622 amino acids), the iron–sulfur protein
(Ip, 29 kDa, 252 amino acids), and two membrane-
anchor proteins (CybL, 15 kDa, 140 amino acids, and
CybS, 11 kDa, 103 amino acids) with a total of ... Lane 1, nucleotide acid marker
DL2000 (TaKaRa); Lane 2, CybS subunit. The
FP band is not very clear in the agarose gel,
and the longest band was used to run
another PCR reactio...
... increase in malonyl-CoA promotes a decrease
in neuropeptide Y and agouti related peptide in hypo-
thalamic malonyl-CoA while promoting an increase in
proopiomelanocortin and cocaine and amphetamine
regulated ... accompanied by a suppression in energy
expenditure. These data suggest that CPT1c can
integrate carbohydrate and lipid nutrient sensing in the
brain and...
... in
A. thaliana PS. Val111 and Gly113, on the other hand,
are both affected by both pantoate and ATP. These
residues have their positional equivalents in Val137 and
Arg139 in A. thaliana PS, as ... to
D-pantoate and ATP are show-
n in green. The arrows indicate the sign of
change in chemical shift of the panto-
ate-bound (P; in blue) and pantoate +
ATP-bound...
... affinity, and incorporation
of an active site inhibitor into FVIIa and G37 2A-
FVIIa increased the affinity for sTF in an indistin-
guishable manner. The differences and similarities
between G37 2A- FVIIa ... region and the S1
pocket, whereas the intramolecular connections, for
instance between the TF-binding region and the active
site and between the TF-bindi...
... bifurcation and
the appearance of an unstable limit cycle. The unstable
and stable limit cycles collide and annihilate in a
global bifurcation near the oscillatory borders.
Saturability and consequent ... the SH2 and kinase domains at the back of
the small lobe, preventing the formation of a produc-
tive catalytic cleft [29]. Thus, these interactions cl...
... polyclonal anti-
bodies raised against each of the WhiB proteins. The absorbance
obtained in the positive control (where the antibody used was
raised against that particular antigen) was set to ... The other impor-
tant point is that although the WhiB proteins were
predicted to attain a a-helical structure [16], WhiB5
and WhiB6 appeared to have significant amounts of
b st...
... cultivated around the world,
including the oilseeds rapeseed and canola (Bras-
sica napus and Brassica rapa) and vegetables such ase
cabbage (Brassica oleraceae var. capitata), cauliflower
(B. oleraceae ... out using integration of peak areas
of 3-indolylmethanamine and a calibration curve of
indolyl-3-methanamine (3).
Kinetic analysis and protein measurements
Kinetic par...
... zinc-containing enzymes catalyzing the
reversible hydration of CO
2
to bicarbonate. Ubiqui-
tous in a wide range of eukaryotic organisms, they are
also widespread in the Archaea and Bacteria domains
[11]. ... sections
of the branchial plume and of the trophosome as
shown in Fig. 2A. The branchial plume is composed of
a central obturaculum, mainly made of ex...
... using first the 5¢-pri-
mer 5¢-GGGATTCAAGCCTGGGGAGAAATC-3¢ and
the 3¢-flanking T7 terminator and subsequently the 3¢-mega-
primer and the 5¢-flanking for the T7 promoter. After
amplification, all ... such
as reductants and ROS. An imbalance between these
dual functions is probably associated with many
human pathologies, such as thyroid tumors, breast
and lung cancer, Alzhe...