Báo cáo khoa học: In situ proton NMR analysis of a-alkynoate biotransformations ppt

... In situ proton NMR analysis of a-alkynoate biotransformations From ‘invisible’ substrates to detectable metabolitesLothar Brecker1,*, Julia Petschnigg1, Nicole Depine´1, Hansjo¨ ... and analysis of the fermentation timecourses [11–15]. While, except in propynoate, the triplebond in a-alkynoates do not carry a proton, the substratesare ÔinvisibleÕ in 1H -NMR. The incorporation ... compounds. To get a deeperinsight into such biotransformations in situ 1H -NMR analysis in 1H2O is a valuable analytical method, althoughthe substrates themselves are often ÔinvisibleÕ. Severalmetabolites...

Báo cáo khoa học: Computer-assisted mass spectrometric analysis of naturally occurring and artiﬁcially introduced cross-links in proteins and protein complexes potx

... rigid protein breaks up into MALDI-MS detectable frag-ments, leaving the disulfide bonds intact. We also show the mapping of anumber of chemically introduced cross-links in the NK1 domain of hepato-cyte ... major tool in thestructural analysis of proteins and protein complexesand in large scale analysis of the function of genes(proteomics) [1].For mass spectrometric analysis, the proteins andproteomes ... procedures for the analysis of naturally occur-ring cross-links, e.g. disulfide bonds, and artificiallyintroduced cross-links in proteins. Cross-links imposedistance constraints on amino acid residues...

Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf

... of a JC virus pentanucleo-tide repeat element binding protein: cellular nucleic acidbinding protein. Virus Res 58, 73–82.6 Flink IL & Morkin E (1995) Alternatively processedisoforms of ... November2006)doi:10.1111/j.1742-4658.2006.05596.xThe zinc-ﬁnger cellular nucleic acid binding protein (CNBP) is a strikinglyconserved single-stranded nucleic acid binding protein essential for normalforebrain formation during mouse and ... CNBPproteins with the 5 ¢ UTR of L4 ribosomal proteinmRNA. J Mol Biol 281, 593–608.15 Flink IL & Morkin E (1995) Organization of the geneencoding cellular nucleic acid-binding protein. Gene...

Báo cáo khoa học: Identiﬁcation and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss) doc

... c.secombes@abdn.ac.ukAbbreviations: IL, interleukin; IL-1Ra, interleukin 1 receptorantagonist; ICE: interleukin 1b converting enzyme; IL)18 BP,interleukin 18 binding protein; IFN, interferon; c-IP10, interferongamma induced ... Cloning and expression of interleukin-18binding protein. FEBS Lett. 445, 338–342.32. Novick, D., Kim, S.H., Fantuzzi, G., Reznikov, L.L., Dinarello,C.A. & Rubinstein, M. (1999) Interleukin-18 ... important role in regulating IL-18activities in rainbow trout.Keywords: interleukin 18; alternative splicing; expression;rainbow trout. In the last few years, major advances have occurred in thediscovery...

Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx

... Aboligomerization [22–25]. Since a functioning zinc-bind-ing domain may be present in the Ab sequence of C99,we hypothesized that zinc may affect the oligomeriza-tion state of C101-3FLAG. Therefore, ... APPintracellular domain (AICD or e-CTF) by cleaving 9–7amino acids from c 40 and 42 sites at the e site [1–3].Several lines of evidence support the pathogenic role of c-cleavage of APP in Alzheimer’s ... detection of COS7-SPC99-3FLAG in vitro c-secretase activityWhile performing control reactions for inhibitorexperiments, a two- to ﬁvefold increase in the detection of products arising from in vitro...

Báo cáo khoa học: Genomic structure and expression analysis of the RNase j family ortholog gene in the insect Ceratitis capitata pptx

... manifested by the presence of multiple ARE-binding proteins, including stabilizingproteins [29,30] or proteins that promote degradation of mRNAs that contain AREs [31]. Recent ﬁndingsreveal that the ... organization of the ORFregion of the RNase j family genes is shown in Fig. 7A. In all organisms examined, the region codingfor RNase j is interrupted by two introns, with theexception of the sea urchin ... the boxes indicate the size of the exons, and those belowthe lines indicate the size of the introns. (B) Multiple amino acid sequence alignment of the RNase j family orthologs. Amino acid sequencesare...

Báo cáo khoa học: Experimental and steady-state analysis of the GAL regulatory system in Kluyveromyces lactis docx

... that of the other species whilstretaining the parameters of the former. The in silicore-engineering study indicated that the leaky behavior in response to glucose inhibition was also obtained in S. ... requirement of Gal4p for the expression of geneswith two binding sites is a result of the strong cooper-ativity during the binding of the transcriptional activa-tor to the two binding site genes in ... protein expression of genes with one (full line, f1p) and two (broken line,f2p) binding sites for varying KlGal4ptconcentration in a K. lactis mutant strain lacking KlGAL80.GAL system in...

Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase potx

... display),increasing the likelihood of correct folding in a redu-cing environment while minimizing the risk of proteo-lytic degradation. The association of plasmid andfusion protein can be maintained ... mechanism of the a subunit of tryp-tophan synthase: an amino-terminal autonomousfolding unit controls several rate-limiting steps in thefolding of a single domain protein. Biochemistry 38,10205–10214.45 ... bound-aries of independent folding domains in the a subunit of tryptophan synthase, a b ⁄ a barrel protein. Protein Sci 8,1200–1209.34 Jasanoff A, Davis B & Fersht AR (1994) Detection of an intermediate...

Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx

... cytokines; interleukin; innate immunity; ﬁsh;expression analysis. Cytokines play a signiﬁcant role in initiating and regulatingthe in ammatory process, which is an important defensesystem in innate ... immunity. Cytokines are subdivided intofamilies such as interleukins (ILs), lymphokines, growthfactors, interferons (IFNs) and chemokines. IL-10, initiallyknown as cytokine synthesis inhibitory ... normalizing against products of b-actin. The x-axis indicates the time-periods of LPS incubation and the relative expression of thecarp IL-10 gene on the y-axis. Controls for 0, 1, 3 and 6 h of incubation...

Tài liệu Báo cáo khoa học: A differential scanning calorimetry study of tetracycline repressor pptx

... results in inducerbinding in the protein cavities [33]. These studies show thatsubstitution of protein residues engaged in hydrogenbonding with [Tc–Mg]+results in reduced binding of theinducer ... rate of 1 KÆmin)1(Figs 1 and 2).Therefore, under these experimental conditions, binding of Tc causes an increase in the Tmax of the protein of % 10.0 °C. The ligand binding leads to a doubling ... a domain possessing a helix–turn–helix motif and a domain responsible for binding tetra-cycline. Binding of tetracycline in the protein pocket isaccompanied by conformational changes in TetR,...

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