Báo cáo khoa học: Identiﬁcation and functional expression of a second human b-galactoside a2,6-sialyltransferase, ST6Gal II docx

... HepG2cDNA library (C. Baisez and A. Harduin-Lepers, unpublished data), as the template and two speciﬁc primers For 6I 5¢-CGATGAATTCGTTAACGCTCATCACCATCACCATCACGGGAAATTGGCCATGGGGT-3¢ containing a HpaIsiteandBack6I ... kidney,thymus, liver), and rather weakly in placenta, lung, aorta,amygdala, occipital and parietal lobe and salivary gland.Almost no expression was observed in fetal lung and heart,uterus, bladder, kidney, ... the annealing of the two following synthetic oligonucleotides For EGT 5¢-GATCCGCCACCATGACCATCTTATGTTGGCTCGCTCTCCTGAGCACACTCACAGCTGTTAACGCTGACATCA-3¢ and Back EGT 5¢-GATCTGATGTCAGCGTTAACAGCTGTGAGTGTGCTCAGGAGAGCGAGCCAACATAAGATGGTCATGGTGGCG-3¢....

Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... 275–283.10 Kuwada M, Teramoto T, Kumagaye KY, Nakajima K,Watanabe T, Kawai T, Kawakami Y, Niidome T,Sawada K, Nishizawa Y et al. (1994) Omega-agatoxin-TK containing D-serine at position 46, but ... crosspeaks were assigned and inte-grated, with concomitant cycles of structure calcula-tions for evaluation of distance and angle constraintviolations as well as assignments of additional peaksbased ... D-amino acid in peptide link-age by an enzyme from frog skin secretions. Proc NatlAcad Sci USA 102, 4235–4239.33 Shikata Y, Watanabe T, Teramoto T, Inoue A, Kawakami Y, Nishizawa Y, Katayama K &...

Tài liệu Báo cáo khoa học: Puriﬁcation and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt

... ATCCTCACGAACAAGCAG5RACR GATCGCGATGCAGGCCTTFLF1 GGACGACTACAGCGTCTTCAGTAGAFLR1 TCCAAACAGTCAGTTTCTTAACCGTTable 1. Purification of cellulase from abalone Haliotis discus hannai. Oneunitofcellulasewasdefinedastheamountofenzymethatliberatesreducing ... RTARAAYTGNCCNGCRTCYTGLP6 WAVEQMNYILGDNK R2 CATYTGYTCNACNGCCCAYTTLP7 AWAWALGWDDKLP8 GYHENALP9 WPLDYFLF2 GCCACACTTCTGTCAACATCC3RAC TTCTTCAAGGGCTGGCTCCCT3AP CTGATCTAGAGGTACCGGATCC5RACF ATCCTCACGAACAAGCAG5RACR ... Purification and cDNA cloning of a cellulase from abaloneHaliotis discus hannaiKen-ichi Suzuki, Takao Ojima and Kiyoyoshi NishitaLaboratory of Biochemistry and Biotechnology, Graduate School of...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

... CCATGTCTGCAGATGGTCGAGG 5¢-RACEZf5¢stat6-R2 GGACTGACATTGCTCCAGAGC 5¢-RACEZf3¢stat6-F3 GCTTCAGTGACTCAGAAATTGG 3¢-RACEZf3¢stat6-F4 GTCCAGAATATTCAGCCTTTCACC 3¢-RACEZftbet-F1 CTCCCTCAAACAAACCAGAGTC Initial PCRZftbet-R1 ... CTGCTTTTCTGGGGACTTCA Initial PCRZf3¢foxp3-F1 TGAAGTCCCCAGAAAAGCAG 3¢-RACEZf3¢foxp3-F2 GTGCTTTGTGCGTGTTGAAG 3¢-RACEZf5¢foxp3-R1 TGTATGATGGAAAAGGTGGCA 5¢-RACEZf5¢foxp3-R2 GGAACACACAGAGGGGATGATA 5¢-RACEOligo ... GGAAACTTCCTGTCTCATCCAGTG 5¢-RACEZffoxp3-F1 GGAACACACAGAGGGGATGATA Initial PCRZffoxp3-R1 CTTCAACACGCACAAAGCAC Initial PCRZffoxp3-F2 TGCCACCTTTTCCATCATACA Initial PCRZffoxp3-R2 CTGCTTTTCTGGGGACTTCA...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

... transcriptional co-activator P ⁄ CAF potenti-ates TGF-beta ⁄ Smad signaling. Nucleic Acids Res 28,4291–4298.39 Kahata K, Hayashi M, Asaka M, Hellman U,Kitagawa H, Yanagisawa J, Kato S, Imamura ... DeMarco R, Martins EA,Guimaraes PE, Ojopi EP, Paquola AC, Piazza JP,Nishiyama MY Jr, Kitajima JP, Adamson RE et al.(2003) Transcriptome analysis of the acoelomate human parasite Schistosoma ... day and 35 day para-sites, adult worm pairs, separated adult female and male worms, and eggs. cDNA from uninfected B. glabrata snails served as a neg-ative control.J. M. Carlo et al. SmSmad1B,...

Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc

... GAGAATTCAAAAGAAAGCGGGGAAGAA Construction of pOpacWhW12 CGAGATCTTGTGGACATGGTCCCGTTTC Construction of pOpacWhS1 CGGAATTCGCTAATGGGAGGAAATCAC Construction of pOpacShS2 TACAGATCTTTTCGTCTTGCGAATTTC ... of sipWW7 TTGTGTAAAAGTGATGACATCGCC Cloning of sipWW8 GTGATCCCGATTATTCTGTGTGTT Cloning of sipWW9 GGCGATGTCATCACTTTTACACAA Cloning of sipWW10 AACACACAGAATAATCGGGATCAC Cloning of sipWW11 GAGAATTCAAAAGAAAGCGGGGAAGAA ... of sipVV4 GCCAAAACAACGATAAGCACGCC Cloning of sipVV5 GGATTCATGCTGATTCCTTCGAC Cloning of sipVV6 ACTTGGCACTACACCGCACCTCATGCG Cloning of sipVV7 ATTTCGTGATTGGCGACAACCGC Cloning of sipVV8 GAGAATTCCGGAGGGGGACAGGAATCTTG...

Báo cáo khoa học: Identiﬁcation and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot

... DR2:5¢-CCGTAAGGTCACAAGGTCACTCG-3¢,DR3:5¢-CCGTAAGGTCACAGAGGTCACTCG-3¢, DR4: 5¢-CCGTAAGGTCACAGGAGGTCACTCG-3¢, DR5: 5¢-CCGTAAGGTCACCAGGAGGTCACTCG-3¢. PAL0: 5¢-CGCAAGGTCATGACCTCG-3¢. One strand of each oligonucleotide ... bp wasproduced by PCR ampliﬁcation with TOPO 2.1-SmNR1 as a template (forward primer: 5¢-ATTTCAGAAGTTGAACAAACACAC-3¢, reverse primer: 5¢-AAGATGGTATTGAAGATGATGGTTGA-3¢), puriﬁed from agarose ... Extraction kit (Qiagen, Valencia, CA, USA) and randomly labeled with32P using a Metaprime kit (Amer-sham Pharmacia Biotech Inc., Piscataway, NJ, USA). ForBAC DNA sequencing, the BAC clone was...

Báo cáo khoa học: Identiﬁcation and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf

... 25–29.12 Kawakami R, Sakuraba H, Kamohara S, Goda S,Kawarabayasi Y & Ohshima T (2004) Oxidative stressresponse in an anaerobic hyperthermophilic archaeon:presence of a functional peroxiredoxin ... & Garrett RA (2005) Diver-gent transcriptional and translational signals inArchaea. Environ Microbiol 7, 47–54.17 Bell SD (2005) Archaeal transcriptional regulation – vari-ation on a ... the ﬁrst of all Prxs analysedso far in archaea that has only one cysteine residue inthe sequence.Transcriptional analysis of bcp2 under oxidativestress and characterization of mRNA 5¢ endIn...

Báo cáo khoa học: Identiﬁcation and characterization of B¢¢-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues Evidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein pot

... tttcagACCCTA8 157 ACCAGGgtaagt 5461 atttagCTTCAT9 49 AACAAGgtaaga 2646 ctttagGGGAAA10 90 TACCAGgtatga 11341 atctagCATTGA11 177 TTGATGgtgaga 1075 atgtagGCAAAA12 119 CAGAAGgtaaca 2839 aatcagGATGTT13 ... the primers5¢-GTTCCTGAGCAAAGTCTTCAATG-3¢ and 5¢-GGAATTCAGTGGAAAAACTTTACAT-3¢ for XN73, and the primers 5¢-GGAATTCATGCCGACCACAACCGTTTTAAG-3¢ and 5¢-GGAGACAGTGACAAGTTATCTAGTGCT-3¢ for XPR70. ... ACACAGgtttga 3629 ttttagATTCAA3 267 GCAAAGgtaatg 13760 ttgcagGTCTGT4 104 GAGAAAgtaagt 8296 ttatagGTTGCT5 103 CTTCAGgtaatt 185761 tttcagGATGTG6 75 ACCACGgtaggc 7814 aaccagGTTATT7 87 TTGCAAgtatgc...

Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

... shortest functional domain from a crenarchaeal plasmid endowed withDNA and RNA synthesis and terminal transferase activity.AbbreviationsAEP, archaeo-eukaryotic replicative primases; dNTP, deoxyribonucleotide; ... multifunctional nature, archaealDNA primases share a number of features with eukar-yal ones and are consequently subsumed within thesuperfamily of structurally related proteins calledarchaeo-eukaryotic ... calledarchaeo-eukaryotic replicative primases (AEPs) [11].Primase–polymerases (prim–pols) are a novel family of AEPs which are sporadically found in both bacterio-phages and crenarchaeal and Gram-positive...

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