Báo cáo khoa học: Plasminogen activator inhibitor type-1 inhibits insulin signaling by competing with avb3 integrin for vitronectin binding pptx
Báo cáo khoa học: Plasminogen activator inhibitor type-1 inhibits insulin signaling by competing with avb3 integrin for vitronectin binding pptx
... PRIORITY PAPER Plasminogen activator inhibitor type-1 inhibits insulin signaling by competing with avb3 integrin for vitronectin binding Roser Lo´pez-Alemany1, Juan ... between integrins and growth factorreceptors has been reported for several systems, one of whichis the modulation of insulin signaling by avb3 integrin. Plasminogen activator inhibitor type-1 ... (PAI-1), competes with avb3 integrin for vitronectin (VN) binding. Here wereport that PAI-1, in a VN-dependent manner, prevents thecooperation of avb3 integrin with insulin signaling inNIH3T3...
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Báo cáo khoa học: A novel inhibitor of indole-3-glycerol phosphate synthase with activity against multidrug-resistant Mycobacterium tuberculosis pptx
... by reducing the binding affinity for substrate of residues Glu168and Asn189. These results suggest that ATB107 is a novel potent inhibitor of IGPS, and that IGPS might be a potential target for ... high-affinity inhibitor, ATB107, of mIGPS, using the virtual screeningapproach. The inhibitor was found to be a competi-tive inhibitor of mIGPS, as it reduced the binding affinity for substrate ... the binding ability of ATB107 was well correlated with itsconcentrations. The equilibrium dissociation contantFig. 4. Ligands with low docking energy values binding to theregion surrounded by...
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Báo cáo khoa học: Mechanistic investigation of a highly active phosphite dehydrogenase mutant and its application for NADPH regeneration pptx
... order was established, with thecofactor binding first and sulfite binding second. The low dissociation con-stant for the cofactor product NADPH combined with the reduced values for DV and kcatimplies ... andDV ⁄ K for both substrates as presentedin Table 2.TheDV value for PTDH-E175A ⁄ A176R was2.21 ± 0.03 with NAD and 1.7 ± 0.1 with NADP.Therefore, the hydride transfer step for the PTDH-E175A ... approximately fourfoldfaster with PTDH-E175A⁄ A176R than with either WT PTDH or aNADP-specific Pseudomonas sp.101 formate dehydrogenase mutant with atotal turnover number for NADPH of 2500.AbbreviationsDH,...
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Báo cáo khoa học: Activated transglutaminase from Streptomyces mobaraensis is processed by a tripeptidyl aminopeptidase in the final step pptx
... be consistent with such a model.Formation of TGase isoforms at distinct differentiationstages is being investigated.AcknowledgementsThis work was supported by the Deutsche Forschungsgemeinschaft ... °Cfor30minwith20lL(1 pmol) TAMEP, 500 lL (20 U) immobilized chymotryp-sin or 250 lL(5U)immobilizedtrypsinin50mMTris/HCl,pH 7.0. Immobilized proteases were removed by centrifu-gation before ... against SM-TAP. For residual activitymonitoring, 70 lL (about 10 lg) of the enzyme was preincubated in 50mM Tris/HCl, pH 7.0, with 10 lL inhibitor and 20 lL ethanol at roomtemperature for 30 min....
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Báo cáo khoa học: Substrate and inhibitor specificity of Mycobacterium avium dihydrofolate reductase pptx
... was37 lm for the D31A mutant and 198 lm for the D31Lmutant, as compared to 0.70 lm for p807. This corres-ponds to a 51-fold increase for the D31A mutant anda 283-fold increase for the D31L ... enzyme assay for determining functionality of theDHFR mutant forms was performed as described previ-ously [4,5]. Each enzyme sample was measured multipletimes. A control reaction without FAH2was ... reaction mix-ture was incubated for 1 min at 30 °C, but with NADPHand FAH2. The reaction was initiated by addition of theenzyme, and the activity was measured, but for 1 min at10 s reading intervals....
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Báo cáo khóa học: The proteasome inhibitor, MG132, promotes the reprogramming of translation in C2C12 myoblasts and facilitates the association of hsp25 with the eIF4F complex pptx
... X-100 for 5 min,rinsed once in NaCl/Pithen incubated with 0.1% (w/v)NaBH4 for 5 min prior to three w ashes with NaCl/Pi. Non-specific binding was blocked by adding 1% BSA in NaCl/Pi for ... for 8 h (middle panels) or incubated for 1 h with 20 lMSB203580 then 8 h with 50 lMMG132 ( lower panels) beforebeing processed as in Fig. 4. The localization of the endogenous proteins within ... or incubated with 5 0 lMMG132 for 8 h(lower panels) before being fixed in 4% (v/v) paraformaldehyde for 15 min and permeabilized in NaCl/Picontaining 0.1% (v/v) Triton X-100 for 5 min. Rabbit...
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Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx
... of S.W. wassupported by the Deutsche Forschungsgemeinschaft(We2843 ⁄ 2-1) and BGAG-Stiftung Walter Hesselbach.The work of S.B. is supported by grants from the Deut-sche Forschungsgemeinschaft ... CagL, CagY and CagA, which by itself binds the host cell integrin member b1followed by delivery ofCagA across the host cell membrane. A role of CagA interaction with phosphatidylserine has ... paradigm for chronic infections. Studies ofH. pylori have revealed not only its ability to colonizeindividual hosts for many decades, but also that thisbacterium has co-existed with humans for...
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Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf
... fixed with 3.7% paraformal-dehyde, quenched with 50 mm ammonium chloride for 15 min, and permeabilized with 0.1% Triton X-100 for 4 min, before being treated with 1.5 m hydrochloric acid for 10 ... detected by Nile red stain-ing as previously described [42]. Cell imaging for Nile redstaining was performed by confocal microscopy. Quantifica-tion of lipid droplets was performed by flow cytometricanalysis ... coverslips for confocal microscopy, or in six-well plates for flow cytometry. After FFA treatment, cellswere fixed with 3.7% paraformaldehyde for 10 min, washedtwice, and stained with Nile red...
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Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
... firstincubated for 24 h in 100 mm KCl to allow for quad-ruplex formation and then with GST-tag-digestedEWS for 1 h at room temperature. The EWS–DNAcomplexes were resolved by 6% PAGE and visualized by ... G-quadruplex binding specificity by ––EWSEWSssDNA LETS-1ssDNA SHtelodsHteloBA––EWSEWS–EWSFig. 1. Affinity of EWS for binding to G-quadruplex DNA. (A) EMSAwas performed with EWS (lanes ... as an equilibrium G-quadruplex forma-tion of some antiparallel form of the hybrid paral-lel ⁄ antiparallel (3 + 1) form together with the parallelpropeller form and the basket type [37–42]....
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Tài liệu Báo cáo khoa học: The Saccharomyces cerevisiae vacuolar acid trehalase is targeted at the cell surface for its physiological function docx
... (A,B) BY4 742 ath1D trans-formed with pGFP–ATH1 under the MET25promoter in YN glucose without methionine.(A) (left) fluorescence, (right) DIC. (B) Immu-noblot with anti-GFP of crude extract before()) ... crude extract before ()) andafter (+) deglycosylation with EndoH usingDsRed polyclonal antibody (C). (D) BY4 741cells transformed with pSPPEP4–ath1DN–mCherry were collected for live cell micros-copy. ... (0.17%w ⁄ v yeast nitrogen base without amino acid and withoutammonium, supplemented with 0.5% ammonium sulfatew ⁄ v, buffered to pH 4.8 with sodium succinate ⁄ NaOH and with the auxotrophic amino...
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