... 13 and 23 contain nonspecific competitor. Lanes 4, 14 and 24 contain DR0 ascompetitor. Lanes 5, 15 and 25 contain DR1 as competitor. Lanes 6, 16 and 26 contain DR2 as competitor. Lanes 7, 17 and ... of a target gene, and a conserved ligand-binding domain(LBD) that is involved in transcriptional activation of the target gene via ligand and coregulator binding.Some NRs have no known ligand ... controls. Lanes 2, 6, 10, 14,18, 22 , 26 and 30 contain lysate with in vitro translated SmNR1. Lanes 3, 7, 11, 15, 19, 23 , 27 and 31 contain lysate with in vitro translatedSmNR1 and SmRXR1. Lanes...