Báo cáo khoa học: The 3¢-UTR of the mRNA coding for the major protein kinase C substrate MARCKS contains a novel CU-rich element interacting with the mRNA stabilizing factors HuD and HuR ppt
Báo cáo khoa học: The 3¢-UTR of the mRNA coding for the major protein kinase C substrate MARCKS contains a novel CU-rich element interacting with the mRNA stabilizing factors HuD and HuR ppt
... TCT TTCTTT CTT TCT TTC TTT TTT TTT TTC TCG AGCCCC-3¢;antisense:5¢-GGG GCT CGA GAA AAA AAAAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA AGA AAG AAA GAA AGG AAT TCG GGCCCG GGG-3¢) representing base pairs ... for the major protein kinase C substrate MARCKS contains a novel CU-rich element interacting with the mRNA stabilizing factors HuD and HuR Georg Wein1, Marek Ro¨ ssler1, Roland Klug1 and ... ARE-containing mRNAs [68] and has beenassociated with the activation of c- Jun N-terminal kinase (JNK) [69]. Similarly, activation of MAP kinase- activated protein kinase 2 has been associated with the stabilization...
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Báo cáo khoa học: SAF-3, a novel splice variant of the SAF-1/MAZ/Pur-1 family, is expressed during inflammation pptx
... splice acceptor 1A 75 ATCTTCCAGgtaacaac 625 cacctcagGGTCACGCC 1C 87 CCATTCCAGgtgagtag 84 ctccgcagGCCGCGCCG2 851 CTTCTCCCGgtgtgcac 403 gtccccagGCCGGATCA364AATGTGAGgtaggaag 277 ctcctcagAAATGTGAG4 ... nucleic acid and predictedamino acid sequences of the cDNA encod-ing SAF-3 are shown. The initiator ATGcodon and stop codon are indicated.Transcription factor SAF-3 is expressed during inflammation ... (Promega) and 1000 UÆmL)1AMV reverse transcriptase(Promega), and incubated at 42 C for 90 min. The sample was extracted with phenol/chloroform and ethanol-precipitated. As a negative control,...
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Báo cáo khoa học: Calpain 3: a key regulator of the sarcomere? pot
... substrates.Through the absence of cleavage of these substrates, calpain 3 deficiencyleads to abnormal sarcomeres, impairment of muscle contractile capacity, and death of the muscle fibers. These data indicate a ... Kroupova I, VondracekP, Marikova T, Mazanec R, Zamecnik J, Stanek J,Havlova M & Fajkusova L (2004) Mutations in CzechLGMD 2A patients revealed by analysis of calpain3 mRNA and their phenotypic ... activity of calpain 3As a cytoplasmic protease, the activity of calpain 3must be tightly regulated temporally and spatially tobe effective and to avoid unwanted damage. Besidescontrol at the transcriptional...
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Báo cáo khoa học: N-Glycan structures of squid rhodopsin Existence of the a1–3 and a1–6 difucosylated innermost GlcNAc residue in a molluscan glycoprotein pot
... of the Japan Science and Technology Corporation, the Yamada Science Foundation, and the Mizutani Foundation for Glycoscience. K.K.H. and H.H.H. are supported financially by the Academia Sinica, ... which was confirmed by cochro-matography of the digestion product of glycan B1 with glycan C on the ODS and amide-silica columns. On the basis of these data, we conclude that glycan C is an analog of ... Mana1–3(Mana1–6)Manb1–4GlcNAcb1–4GlcNAc. In contrast,octopus rhodopsin [14] expresses a unique N-glycan struc-ture which contains a characteristic Galb1–4Fuca1–6branch attached to the reducing terminal GlcNAc....
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Báo cáo khoa học: Structure and function of the 3-carboxy-cis,cis-muconate lactonizing enzyme from the protocatechuate degradative pathway of Agrobacterium radiobacter S2 pdf
... C- terminal His-tagged enzyme, as follows[23]. The DNA segments encompassing pcaB1S2 wereamplified by PCR using the primers CMLEI-X-N (ATAACA TAT GAG CCT TTC CCC CTT CGA AC) and CMLEI-His -C (AAA ... in the protoca-techuate branch of the 3-oxoadipate pathway of various aerobic bacteria. The gene encoding a 3-carboxy-cis,cis-muconate lactonizing enzyme(pcaB1S2) was cloned from a gene cluster ... FEBSStructure and function of the 3-carboxy-cis,cis-muconatelactonizing enzyme from the protocatechuate degradativepathway of Agrobacterium radiobacter S2Sad Halak1,*, Lari Lehtio¨2,3,*, Tamara...
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Tài liệu Báo cáo khoa học: Glycomics-based analysis of chicken red blood cells provides insight into the selectivity of the viral agglutination assay docx
... completed additional MS and NMR-based analysis of the cRBC glycan pool. Inaddition to identification and quantification of othermonosaccharides, we aimed to characterize the overallsialic acid content, ... GlcNAc-5, GlcNAc-5¢, galactose (Gal)-6,Gal-6¢ and Gal-8 appear in the range 4.40–4.75 p.p.m.(Table S1). Specifically, the anomeric proton of GlcNAc-2 appears at 4.62 p.p.m.; the GlcNAc-5 and GlcNAc-5¢ ... hemagglu-tination assay takes advantage of the fact that hemag-glutinin (HA) on the surface of human-adapted virusestypically binds to speci c sialylated glycans on the surface of epithelial cells of the...
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Tài liệu Báo cáo khoa học: 3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-c-dependent and mediated by the caspase-3-dependent apoptotic pathway doc
... 693TTTTTCAGTGCAGAA-3¢; aP2 forward, 5¢-AAAGACAGCTCCTCCTCGAAGGTT-3¢; and aP2 reverse, 5¢-TGACCAAATCCCCATTTACGC-3¢. Standard curves were gen-erated with 10-fold serial dilutions ranging from ... against actin in the same sample. The PCR primers were as follows: actin forward, 5¢-GAAATCGTGCGTGACATCAAAG-3¢; actin reverse, 5¢-TGTAGTTTCATGGA TGCCACAG-3¢; Akt-1 forward, 5¢ -A ACGGACTTCGGGCTGTG-3¢; ... 5¢ -A ACGGACTTCGGGCTGTG-3¢; Akt-1 reverse, 5¢-TTGTCCTCCAGCACCTCAGG-3¢; CD36 forward, 5¢-TCCAGCCAATGCCTTTGC-3¢; CD36 reverse, 5¢-TGGAGATTAC A BEFGCDFig. 5. Inhibition of troglitazone (Tro)-induced 3T3-L1 adipocyte...
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Tài liệu Báo cáo khoa học: Minor capsid proteins of mouse polyomavirus are inducers of apoptosis when produced individually but are only moderate contributors to cell death during the late phase of viral infection ppt
... caspase 3 activity and the caspase inhibition assayAt indicated time-points, cell lysates were prepared and tested for cleavage of amino acid DEVD sequences by cas-pase 3 using the CaspACE assay system, ... polyoma-virus capsid proteins that serve as nuclear localizationsignals. Trans Kans Acad Sci 96, 35–39.5 Moreland RB & Garcea RL (1991) Characterization of a nuclear localization sequence in the ... variants of the minor proteins or EGFP only, mock-transfected cells, cells treated with actino-mycin D (ActD), or untreated cells. (A) Cleavage of caspase 3 and of PARP in lysates of cells collected...
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Tài liệu Báo cáo khoa học: An autoinhibitory effect of the homothorax domain of Meis2 ppt
... 5¢-GAGGTCGATGGGCATTTTC-3¢;Meis3-F, 5¢-GATGATCCAGCCATCCA-3¢; Meis3-R, 5¢-GGCTGGGTAGTCCTCGAAGT-3¢; mMeis3-F, 5¢-GTCCAGGCCATCCAGGTACT-3¢; and mMeis3-R, 5¢-TCCTCCCTGCAACTACCATC-3¢. The relative ... the conformation or intramolecular interactions, allowingaccess to the AD. As the autoinhibition affected anunrelated AD when this was put in place of the nativeMeis2d AD, it appears that any ... cytoplasmic in the absence or presence of Pbx1 [45]. Thus the nuclear ⁄ cytoplasmic localization of Prep1, and possibly of other Meis paralogs, may play a role in regulating transcriptional activity,...
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Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc
... interior of the micelle or the aqueous buffer. Qualitative analysis of secondary chemicalshift and paramagnetic relaxation enhancement data in conjunction with dynamic information from heteronuclear ... and the chemicalshift list reflecting the sequence-speci c resonance assign-ment. Intermediate protein structures are calculated at the end of each cycle and provide additional guidance for the interpretation ... dyana for calculation of preliminary protein structures [79]. radar runs in an iterative manner. The input of the first cycle consisted of the 3D NOESYdata, the amino acid sequence of VpUcyt and...
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Từ khóa: Nghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Thơ nôm tứ tuyệt trào phúng hồ xuân hươngSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀM