... incorporation of TVASS into b-sheet A. Thisobservation is in agreement with the notion that the rate-limiting step during latency transition is not insertion of theRCL into b-sheet A, but rather ... comes from stabi-lization of the serpin in the ÔrelaxedÕ conformation byinsertion of the RCL into b-sheet A, as opposed to theÔstressedÕ, relatively unstable active conformation with asurface-exposed ... hydrolysis,resulting in abortive complex formation, full cleavage ofthe P1–P1¢ bond, insertion of the RCL into b-sheet A andrelease of an active proteinase (for reviews see [17,20]).Serpins following...