... upregulation of bothMCAD and chaperonin. It is clear, however, that,under these more stressful (though physiological) con-ditions of temperature, even the wild-type enzymedepends upon chaperonin for ... Mrvalues(46 590 for wild type, and 46 630 for K364R, the slightvariation due to the mutation) to define the concentrationof active sites. Michaelis–Menten plots were used only todisplay the ... Purifi-cation and characterization of short-chain, medium-chain, and long-chain acyl-CoA dehydrogenases fromrat liver mitochondria: isolation of the holo- andapoenzymes and conversion of the...