... (Fig. 6A,B). TcrPDECexhibits a Km for cAMP of 31.6 ± 9 lm (N ¼ 6). Thisis significantly higher than the Km for cAMP observed for a previously characterized PDE from T. cruzi,TcPDE1 [14]. Unexpectedly, ... an unusual PDE from this protozoal organism. This enzyme, TcrPDEC, is a member of the classI PDEs, as determined from the presence of a characteristic signaturesequence and from the conservation ... LmPDEC, phosphodiesterase from Leishmania major; PtdIns(3)P, phosphatidylinositol-3-phosphate; TbPDEC, phosphodiesterase from Trypanosoma brucei; TcrPDEC, phosphodiesterase from Trypanosoma cruzi.6412...