... 2,4-cyclodiphosphate.Proc. Natl Acad. Sci. USA 97, 2486–2490.23. Takagi, M., Kuzuyama, T., Kaneda, K., Watanabe, H., Dairi, T.& Seto, H. (2000) Studies on the nonmevalonate pathway:formation of 2-C-methyl-D-erythritol ... identified afterdephosphorylation and acetylation by the usual method.The acetylated crude residue was analysed by GCMS, andthe analytical data compared with those of a syntheticreference of deoxyxylulose ... results,methylerythrose phosphate apparently has a good affinity with the enzyme, at least as compared with that of DXP 3Table 1. Determination of the kinetic parameters (Kmand V)ofDXP3and2-C-methyl-D-erythrose...