Báo cáo khoa học: Molecular characterization of H2O2-forming NADH oxidases from Archaeoglobus fulgidus potx

Báo cáo khoa học: Molecular characterization of artemin and ferritin from Artemia franciscana pot

... amino-acid sequence of the arteminmonomer consists of 230 residues with a calculated molecular mass of 25 976 Da.The ferritin cDNA (accession number AY062897) of 725 bp consists of an ORF of 516 bp, ... Canada;2Department of Molecular Cell Biology,Sylvius Laboratory, Leiden, the Netherlands;3Section of Molecular and Cellular Biology, University of California,Davis, Bodega Bay, CA, USA;4Department of Biological ... ferritins, and they form oligomers of similar size [23]. Thus, puriﬁed artemin has a sedimentationconstant of 19S and a molecular mass of 573–610 kDa,probably consisting of 24 subunits. It was suggested...

Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... (A) Scheme of the three-hybrid strategy used inthe screen. (B) Number of colonies identiﬁed in each step of thescreen. (C) Distribution of the 63 distinct sequences in relation of their position ... 3¢ UTR. In recent years, and as a result of great efforts aiming to understand the mechanism of RNA control in animals, the function of a diversenumber of RNA-binding proteins has been elucidated[1–4]. ... 3 out of 25 proteins were found tofall outside these groups. Within each group, someproteins show a degree of primary sequence identity, from 40% to 90% along their entire lengths, andfrom...

Báo cáo khoa học: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae pot

... Munn2,3and Hongyuan Yang11 Department of Biochemistry, Faculty of Medicine, National University of Singapore, Republic of Singapore2 Institute of Molecular and Cell Biology, A*STAR Biomedical ... anti-Osh6pserum from cell extracts of wildtype cells, which wasabsent from extracts of OSH6 knockout cells. On theother hand, the amount of Osh6p protein overexpro-duced using the ADH1 promoter from ... its mature form (M form with molecular mass of 61 kDa). Deﬁciency in this processleads to accumulation of and secretion of p2 CPY. Asshown in Fig. 7, at 0 min of chase, p1 CPY was thepredominant...

Báo cáo khoa học: Molecular characterization of H2O2-forming NADH oxidases from Archaeoglobus fulgidus potx

... that the three NADH oxidases belong to differentphylogenetic clusters. NoxA-1 falls within a group of typical NADH oxidases of  49 kDa, including the well-studied NADH oxidases from Enterococcus ... 1).The physiological role of the putative NADH oxidases inA. fulgidus is still enigmatic. Common NADH oxidases of mesophilic origin are assumed to protect the cells from oxidative stress by reducing ... reactions,such as NADH peroxidase (EC 1.11.1.1) and disulﬁdereductase (EC 1.8.1.14). The noxA homologues from A. fulgidus code for a group of typical H2O-forming NADH oxidases of  49 kDa, found...

Báo cáo khoa học: Molecular characterization of a novel nuclear transglutaminase that is expressed during starﬁsh embryogenesis ppt

... predicted molecular mass of 83 kDa. A comparison of this transglutaminase with other members of the gene familyrevealed an overall sequence identity of 33–41%. A specialsequence feature of this ... of a c-carbonyl of histoneH2B by esteriﬁcation, followed by a nucleophilic attackby an e-amino group of Lys residue of histone H4 [38], thefact that the cross-link is formed between Gln9 of ... function as acomponent of the signal-transducing G protein complex [7].The cDNA of Gha, involved in the transmission of adrenergic stimuli, is identical to that of tissue TG of human endothelial...

Báo cáo khoa học: Molecular characterization of a blood-induced serine carboxypeptidase from the ixodid tick Haemaphysalis longicornis docx

... a molecular mass of 53 kDa and a pI of 7.5 (Fig. 2A),corresponding to the predicted size of the putative pro-tein calculated from the HlSCP1 amino acid sequence.To detect the localization of ... Wereport the molecular characterization and possible function of a serinecarboxypeptidase (HlSCP1) identiﬁed in the midgut of the hard tick Haem-aphysalis longicornis. HlSCP1 consists of 473 amino ... reactivity.Subcellular localization of HlSCP1To clarify the subcellular localization of HlSCP1 in themidgut, immunoblot analysis of the subcellular frac-tions of midgut tissues obtained from 72-h-fed adultfemale...

Báo cáo khoa học: Molecular characterization of recombinant mouse adenosine kinase and evaluation as a target for protein phosphorylation potx

... usingIMAGEQUANTsoftware (Amersham Biosciences). Standardsconsisted of 5 lL aliquots of serial dilutions of the reactionmixtures, with the moles of phosphate deﬁned using theATP c oncentration. Division of ... transfer of the c-phosphate from ATP to the 5¢-hydroxyl of Ado, generating AMP and ADP, is one of severalenzymes responsible for maintaining steady-state Ado levels[6]. The structure of AK has ... mAK-L. To the best of ourknowledge, this is the ﬁrst report of the deduced aminoacid sequence of mAK-S.In order to study the function and regulation of mouseAK in vitro, both isoforms were s ubcloned...

Báo cáo khoa học: Molecular characterization of secretory proteins Rv3619c and Rv3620c fromMycobacterium tuberculosisH37Rv docx

... square ﬁt. The heat of dilution was subtracted from the raw data of titration of Rv3619c with Rv3620c. (B) Far-UV CD spectra of Rv3619c, Rv3620c, and the 1 : 1complex. CD spectra of 5 lM Rv3619c ... is the recipient of research fellowships from the Council of Scientiﬁc and Industrial Research(CSIR), New Delhi, India. S. Srivastava is the recipient of project assistantship from the CSIR network ... pro-ject. The work was supported by grants from CSIRnetwork project NWP0038 and from the Department of Biotechnology. M. A. Ansari is the recipient of afellowship from ICMR.References1 Behr MA, Wilson...

Báo cáo khoa học: Molecular characterization of gonad-inhibiting hormone of Penaeus monodon and elucidation of its inhibitory role in vitellogenin expression by RNA interference pptx

... feature of the amino acid sequences at the C-terminus, which arelonger than and different from that of MIHs. Toobtain the 5¢ region of this cDNA, a set of specificprimers was designed from the ... The identity of RT-PCR products was confirmed by DNA sequencing.Fig. 3. Alignment of Pem-GIH with GIH and MIH from other species of crustacean. The deduced amino acid sequence of GIH from P. monodon ... of Pem-GIHcDNA were designed from the conserved amino acidsequences of MIH ⁄ GIH from several species of crustacean.In the first round of PCR, a 1 lL aliquot of cDNA wasamplified with 3¢-RACE-GIH1A...

Báo cáo khoa học: Kinetic characterization of methionine c-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and triﬂuoromethionine, a promising lead compound against amoebiasis ppt

... at a molecular ratio of 1 : 1000,and further tested for the Met-degrading activity onthe basis of the detection of a-keto acid after the addi-tion of 2 mm Met (Fig. 3). Approximately 85% of EhMGL2 ... Kobayashi S, Ikegami F, Noji M,Saito K & Takeuchi T (1998) Molecular cloning and characterization of the genes encoding two isoforms of cysteine synthase in the enteric protozoan parasiteEntamoeba ... Nozaki11 Department of Parasitology, Gunma University Graduate School of Medicine, Japan2 Department of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology,...

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