Báo cáo khoa học: Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp no 92 pdf

Báo cáo khoa học: Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92 pdf

... oligopeptide sequences and using PCR, the gene of the cyclodextrinase from Flavobacterium sp. no. 92 wasderivedfromthegenomicDNA.Thegenewassequenced and expressed in Escherichia coli; the gene pro-duct ... longer and more complex than the loops at the opposite barrel end. The lengths of the b-strands in the barrel vary from two (b14)to seven (b11) residues. As in other enzymes of family no. 13, the ... [16]. The sequence and 3D structure of the enzyme is presented and comparedwith related proteins.Experimental proceduresIsolation and sequencing of the gene The enzyme was puriﬁed from Flavobacterium...

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... II. The hood domain is mainly builtup by residues from the C-terminus and consists of atwo-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig. 1A). The two ... atoms,whereas the side chain of Lys173 interacts with bothO6 and N7 (Fig. 1C). The 2¢OH of the ribose is hydro-gen bonded to the main chain and the side chain of Asp142 (Fig. 1C). Both hydroxyl groups of ... chain of Asp201, a phosphate ion and ﬁve water molecules. The phosphate involved in this interaction is bound bymain chain interactions with Lys76 and Gly77 and the side chain of Arg207. The phosphate...

Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc

... [57].Isolation and cloning of the SynMGST and E.coliMGST The coding sequence for the SynMGST, corresponding to the complementary strand of the nucleotide sequence from 89 254 to 89 667 in the Synechocystis ... above and in[1]). We investigated representative gene products from the E. coli and Synechocystis sp. bacteria further, togain insight into the function of these proteins and the evolution of the ... band was also observed in fractions from the other puriﬁcation steps. The band was cut out from the gel followed by elution of the protein and the N-terminalamino acid sequence was determined using...

Tài liệu Báo cáo khoa học: Covalent binding to glutathione of the DNA-alkylating antitumor agent, S23906-1 doc

... GSH and to the diol, mono-acetate and di-acetate forms of the parent drug, respectively. The latter three species reﬂect the hydrolysis of the compound in solution (see the resultsbelow with the ... reactivity of S23906-1 towards the bionucleophiles DNA and GSH. The covalent binding of the drug to GSH reduced the formation of DNA adducts and slightly decreased the cytotoxic potential of the molecule. ... ethanol. The ﬂuorescence spectrum of the DNA-bound S23906-1 specieswas then recorded. The results shown in Fig. 6A clearlyreveal that the preincubation of the drug with GSSG had no effect on the...

Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx

... distortion of the )1sugar and by increasing the pKa of the catalytic acid. The hydroxyl group of Tyr214 makes a major contribution to the positioning of the N-acetyl group of the )1sugar.Takentogether, ... simplicity, and in line with the results from the other calculations, the <0.0 value is allocated to Asp140 and > 20.0 to Asp142.b The calculations were performed on the crystal structure of the ... 257rows 5 and 7 shows that in the presence of the substrate,rotation of Asp142 towards Glu144 lowers the pKa of the latter by 0.8 pH units. The calculated effects of the D140N and D215N muta-tions...

Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx

... 7).Although the spectral characteristics of the lac1 proteinsuggest the absence of a type 1 copper site, this is not borneout by analysis of the deduced amino acid sequence of the enzyme. Thus, the ... amino acid sequence from Ala25 to Asp41 corresponded to the N-terminal sequence of the puriﬁedprotein,andtheputativepresequenceof 24aminoacids is a hydrophobic signal peptide as predicted by the computer ... portion of the lac1 protein and RACE was used to obtain full-lengthcDNA clones. The cDNA of lac1 contained an ORF of 1557 bp encoding 519 amino acids. The amino acid sequence from Ala25 to Asp41...

Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx

... comprising the N-terminal fragment and the ﬁrst AAA-cassette of PEX6 (PEX6N + D1–GAL4-BD). Neither of the cas-settes alone nor the two AAA-cassettes together or the N-terminal part together with the ... assigned their corresponding binding sites and elucidated the importance of ATP-binding and -hydrolysis of Pex1p and Pex6p for theirinteraction. We show that the interaction of Pex1p and Pex6p ... Pex5p and Pex7p, respectively[11,12]. These two peroxins bind their cargo in the cytoplasm, transport them to (and possibly across) the peroxisomal membrane and return to the cytoplasmfor the...

Báo cáo khoa học: Spectroscopic and kinetic properties of the horseradish peroxidase mutant T171S Evidence for selective effects on the reduced state of the enzyme potx

... from comparison of the electronic absorption and RR spectra of the wild type and Thr171Ser eitherin the presence of the aromatic donor BHA or atalkaline pH. Addition of saturating amounts of ... amounts of BHAdid not reveal any spectral differences between the wildtype and the mutant (data not shown). Furthermore, the electronic absorption spectra of the Thr171Sermutant and wild-type HRPC ... to that of the wild type, % 11.1[18]. Finally, comparison of the X-ray structures of the oxidized forms of the native [5,19] and the T171Smutant (protein databank code: 1GW2.pdb) did notreveal...

Báo cáo khoa học: Functional and genetic characterization of the promoter region of apolipoprotein H (b2-glycoprotein I) ppt

... vector. The nucleotides are numbered from the translation start site (ATG). The effect of the wild-type and mutants was measured as the mean of the firefly luciferase levels nor-malized by the Renilla ... conjunction with the luciferase gene in pGL3-basic vector. The nucleotides are numbered from the translation start site. The effect of the wild-type and mutants was measured as the mean of the firefly ... tested 12 of the 14 sequence vari-ants located within the 1418 bp of the 5¢ flankingregion of APOH for allele-specific regulatory effects on the expression of the dual luciferase reporter gene and by...

Báo cáo khoa học: Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus – the role of conserved residues in the active site pdf

... Stereo figures of the model of the bound substrate in the AD2 E526A mutant (A) and D524A mutant (B). The structures of boundsugars and the side-chains of three acidic residues in the conserved ... causes the distortion and twist-ing of the substrate, so that the glycosidic oxygen facestowards the bottom of the deep cleft.Enzyme–substrate interactions The crystal structures of the AD2 ... identical (Table 2). In the interest of simplicity, the structures are discussed consistently using the first mono-mer of the coordinate files, unless otherwise stated. The structures and electron density...

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