...
Abstract
This paper introduced the main features of the
UAM CorpusTool, software for human and
semi-automatic annotation of text and images.
The demonstration will show how to set up ... e.g., MMAX-2 (Müller and Strube 2006),
GATE (Cunningham et al 2002), WordFreak
(Morton and LaCivita 2003) and Knowtator
(Ogren 2006).
With the exception of the last of these...
... complex, O2¢
and O3¢ form hydrogen bonds with OE1 of Glu56 and
NE2 of His86, respectively. Arg67 and Arg71 interact
with the oxygens of the phosphate group, and site-
directed mutagenesis studies ... Preparation command of
glide. Interactions of probe atoms with proteins were
calculated with the Receptor Grid Generation command of
V. Bauerova
´
-Hlinkova
´
et al. S...
... containing
4 pmol of each primer, 10 lLof2· SYBR Premix Ex Taq,
and 2 of 20 lL single-strand cDNA reactions. To determine
the absolute copy number of the target transcripts in indi-
vidual cDNA reaction ... exon 7 of fragment C represents a dele-
tion of 39 bp. The thick lines indicate intron 6 of 75 bp in fragments
B and C, 3¢ portion of 35 bp in intron 5 in fragments C...
... HPI, and to further learn the molecular mechanism of the
initiation of HPI folding.
Acknowledgements
We thank Profs M. A. Weiss and Q X. Hua for providing human
proinsulin and helpful ... sequences and in vitro refolding pathway of PIP and
HPI. (A) Amino acid sequences of PIP and HPI. Amino acids are
shown in the one-letter code. The numbering of the residues in HPI
a...
... the presence of Ca
2+
with a
K
D
of 327 ± 112 nm; binding of BD-N and BD-C2
were not altered with labeled with Cy5 instead of
TMR.
In FCS measurements fluorescently labeled peptides
of both, the ... in steps of 3) and C-terminal (amino-acids
480–962 in steps of 3 and amino-acids 645–895 in steps
of 2) cytosolic domains of hEAG1. Binding of
BODIPY FL-labeled hCaM...
... initiating a cascade of detrimental
events for the cell. Interaction of granular aggregates and globular oligo-
mers of an amyloidogenic protein, human stefin B, with model lipid mem-
branes and monolayers ... channel hypothesis of AD and neurodegeneration
in general, is not incompatible with other key elements
of toxicity, as, for example, the deregulation of Ca
2+
hom...
... biphasic,
with the best fit obtained with a two binding site
model, comprising a first site with binding stoichiome-
try (n) of 0.3 ± 0.2 and a binding constant (K
d
)of
3.4 ± 4 · 10
)9
m and a second with ... parameters of the interaction (on
rate constant k
on
and off rate constant k
off
) between
NarJ and the NarG(1–15) peptide. Taking into account
the existence of tw...
... over-expres-
sion of Hsp60 and Hsp70 and exhibit abnormal mor-
phology in correlation with increased proliferation,
premature differentiation and elongation, inhibition of
denucleation and of loss of organelles ... mechanisms for the lack of HSF2. In line with
this hypothesis, double disruption of Hsf1 and Hsf2
is associated with sterility and complete arrest of...
... target, loading of the appropriate guide
strand into the RISC is critical. Improved understand-
ing of the interactions of siRNAs with TRBP and Dicer
will enable improved design of siRNA therapeutics.
In ... more sta-
ble end of the siRNA duplex and directs binding of
Dicer-2 to the other, less stable, end, and hence the
guide strand is selected through interaction of it...
... roles in develop-
ment and in health and disease [1]. Their properties
have been studied by a combination of in vitro analy-
ses of human primary ECs and in vivo analyses of
genetically modified ... concentra-
tion of G418 (Invitrogen) of 400 lgÆmL
)1
. G418-resistant
colonies were picked and expanded for PCR genotyping
and the formation of embryoid bodies. One ESC clon...