... expression vector under the control of the T7promoter, and coexpression of oraE and oraS genes wascarried out in E. co li. Meanwhile, the extent of theinvolvement of AdoCbl or PLP in the in vivo foldingprocess ... temperatures:95 °C for 30 sec, 50 °C for 1 min, and 72 °Cfor4min.Finally, the reaction was maintained at 72 °Cfor5min.The PCR products were gel-purified, restricted with NcoI and BamHI, and ligated ... measuring the apparent Kmvalue of D-ornithine at 25 , 50, 100, 200 and 400 lM of the inhibitor. For the measurement of the activity of the substrateanalogues, an HPLC and NMR-based method was devel-oped....