... with 45 nm VVA1, VVA2 or a mixture of VVA2, 45 nm, and VVA1, 22 .5 nm,at37°C for 30 min in0.02% Triton X-100, 50 mm Tris, pH 8.0. The beads werewashed three times with 50 mm Tris buffer and eluted ... J Biol Chem 270, 147 05 147 11. 24 Lin SC, Lo YC, Lin JY & Liaw YC (20 04) Crystalstructures and electron micrographs of fungal volva-toxin A2. J Mol Biol 343 , 47 7 49 1. 25 Eisenberg D, Weiss ... VVA2 (166–1 94) . (B) Inhibitory effects of VVA1 on the binding of VVA2 to liposomes. Afterincubation of VVA1, VVA2 or the mixture of VVA2 and VVA1 at a molar ratio of 2 with liposomes (5 mM)at37°C...