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Báo cáo khoa học: Protein stabilization by compatible solutes Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR docx

Báo cáo khoa học: Protein stabilization by compatible solutes Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR docx

Báo cáo khoa học: Protein stabilization by compatible solutes Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR docx

... Protein stabilization by compatible solutes Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR Pedro Lamosa1, David L. ... protein known to date [27]. In this context, assessing the changes in the dynamic behaviour of proteins in the presence of solutes is expected to shed light on the stabilization phenomenon. Desulfovibrio ... solutecompletely restricts concerted motions of groups on largertime-scales. The influence of DGP addition on the order parameters of RdDg is of the same magnitude as the effect of ligandbinding reported...
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Báo cáo khoa học: Protein expressed by the ho2 gene of the cyanobacterium Synechocystis sp. PCC 6803 is a true heme oxygenase pot

Báo cáo khoa học: Protein expressed by the ho2 gene of the cyanobacterium Synechocystis sp. PCC 6803 is a true heme oxygenase pot

... FEBSAbsorption spectra of the complexes of heme andHOs resemble the spectrum of myoglobin with oneexception, the HO of Drosophila melanogaster. In the HO of fruit fly, the iron of heme was not ... 3. Absorption spectra of various forms of the Syn HO-2–hemecomplex. The concentration of the complex was 7 lm. The spectraare the iron(III) (solid line), iron(II) (dotted line), iron(II)-CO ... O2and electronsin the conversion of hemin to verdoheme, therebyimplying that the chemistry of heme degradation by Syn HO-2 is similar to that by other HOs. Accord-ingly, we conclude that...
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Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc

Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc

... characterization of the purified enzyme The c oncentrated enzyme solution (fraction 6) was yellow i ncolor. The enzyme s olution showed one main absorptionpeak at 266 nm and a broad absorption band in the ... identified by GC-MS analysis, and the determination of released ammonia. The coupled enzymeassay revealed the mechanism of the deamination reactionand the subsequent metabolism, including the deaminationstep. The ... the deamination step the concentrated crudeextracts of strain 10d grown on 4-amino-3-hydroxybenzoicacid were prepared by ammonia sulfate fractionation(35–75% saturation). Figure 2A shows the...
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Báo cáo khoa học: Caenorhabditis elegans metallothionein isoform specificity – metal binding abilities and the role of histidine in CeMT1 and CeMT2 potx

Báo cáo khoa học: Caenorhabditis elegans metallothionein isoform specificity – metal binding abilities and the role of histidine in CeMT1 and CeMT2 potx

... at the end of the titration, even afteraddition of excess Cu(I), some species still retainedZn(II) ions. The discussion of the Cu(I) coordination behaviorbecomes more complex in view of the ... homonuclear Cu5preparations. Under these conditions, low Zn contentsTable 4. Analytical characterization of the recombinant preparations of the Cu complexes yielded by CeMT1, CeMT2, their N-term andC-term ... provided by Professor J. H. R. Ka¨gi (Institute of Biochemistry of the University of Zurich, Switzerland) andalso used as a template for the construction of regionscoding for the deletion mutant...
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Báo cáo khoa học: A novel metallobridged bis(b-cyclodextrin)s fluorescent probe for the determination of glutathione doc

Báo cáo khoa học: A novel metallobridged bis(b-cyclodextrin)s fluorescent probe for the determination of glutathione doc

... tem-perature.Influence of interference The influence of the main constituents of plasma on the determination of 2.00 · 10)6molÆL)1GSH were studied. The criterion for interference was fixed at a± 5.0% variation ... bis(b-CD)s.Because of the conformation of the linker of 1, the nitrogen atoms and amido bond formed two chelaterings to coordinate with copper (II) ions. This coordi-nation effect and the paramagnetism of ... the fluorescence intensity, 2.00 mL of buffer was chosen insubsequent experiments.Influence of the concentration of metallobridgedbis(b-CD)s 2 The influence of the concentration of 2 on fluorescenceintensity...
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Báo cáo khoa học:

Báo cáo khoa học: "PCFGs, Topic Models, Adaptor Grammars and Learning Topical Collocations and the Structure of Proper Names" ppt

... models. The first replaces the unigram component of LDA topic models with multi-word se-quences or collocations generated by anAG. The second extension builds on the first one to learn aspects of the ... the Doc and Docnonterminals — but ithas the advantage that the LDA distributions mapstraight-forwardly onto PCFG nonterminals). The terminals W of the CFG consist of the vo-cabulary V of ... θSentencespecifies the distri-bution of documents in the corpus. However, it iseasy to see that these distributions do not influence the topic distributions; indeed, the expansions of the Sentence nonterminal...
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Báo cáo khoa học: From functional genomics to systems biology Meeting report based on the presentations at the 3rd EMBL Biennial Symposium 2006 (Heidelberg, Germany) Sergii Ivakhno pot

Báo cáo khoa học: From functional genomics to systems biology Meeting report based on the presentations at the 3rd EMBL Biennial Symposium 2006 (Heidelberg, Germany) Sergii Ivakhno pot

... metabolism. The functional association wasfurther aided by integration of protein interactiondata with data on gene expression, localization, func-tion, evolutionary conservation, protein structure ... than 70% of the promoters are bound by at least one of the top 10%most highly connected TFs. In addition, 82% of the promoters are bound by at least one of the other less-well-connected interactors, ... signal-transductionpathways. One of the key considerations in suchscreens is the choice of appropriate read-out assaysthat can accurately assess the effect of gene knock-down on the pathway of interest [23]....
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Báo cáo khoa học:

Báo cáo khoa học: "Adding Syntax to Dynamic Programming for Aligning Comparable Texts for the Generation of Paraphrases" potx

... (Milan Theater) and is not at the beginning of that constituent. Finally, the syntactic depen-dence trace of a word is the path of IOB valuesfrom the root of the tree to the word itself. The last ... measurement – the relativeposition of the two words in their respective sen-tences. The relative position is defined to be the word’s absolute position divided by the length of the sentence it ... higher than the non-syntactic versions. In particular, Version 4 outper-forms the the best baseline version by 19.9% on grammaticality and by 22.8% on fidelity. Our sec-ond hypothesis is also...
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Tài liệu Báo cáo khoa học: Protein farnesyltransferase inhibitors interfere with farnesyl diphosphate binding by rubber transferase pdf

Tài liệu Báo cáo khoa học: Protein farnesyltransferase inhibitors interfere with farnesyl diphosphate binding by rubber transferase pdf

... The assays were incubatedfor 4 h and were stopped by the addition of 0.5MEDTApH 8 to a final concentration of 20 mM. The incorporated14C was measured by liquid scintillation counting of ... rubbertransferase activity at the final concentration of 10% (v/v)in the rubber transferase assay, so these were the chosenconditions for conducting the inhibitor studies (data notshown).As the chaetomellic ... (not shown) of the slope of the each reciprocal plot vs. the concentration of the inhibitory compound [14] (Table 1).DiscussionRubber transferases exhibit a considerable degree of toler-ance...
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Báo cáo khoa học: Regulated expression by PPARa and unique localization of 17b-hydroxysteroid dehydrogenase type 11 protein in mouse intestine and liver pdf

Báo cáo khoa học: Regulated expression by PPARa and unique localization of 17b-hydroxysteroid dehydrogenase type 11 protein in mouse intestine and liver pdf

... upper).Higher concentrations of fatty acids accelerated the formation of the LDs, but the toxic effects of fattyacids on the cells became evident. Therefore, the cellscultured under normal conditions ... [14], and detected the changesin distribution of the labeled protein before and after the formation of the LDs. To complete pulse-labeling of the Halo-tagged 17b-HSD11 on the ER, the cellswere ... the constructs of Chai et al. might have led to the protein being in the wrong location. We examined the subcellu-lar distribution of 17b-HSD11 in the cells containing the LDs (Fig. 4), and confirmed...
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