Báo cáo khoa học: Two separate regions essential for nuclear import of the hnRNP D nucleocytoplasmic shuttling sequence ppt
Báo cáo khoa học: Two separate regions essential for nuclear import of the hnRNP D nucleocytoplasmic shuttling sequence ppt
... areimportant for the nuclear import mediated by Trn-1.ResultsDetermination of hnRNP D NLS The exon 8 of hnRNP D encodes the 21 amino acids sequence common to all the isoforms D0 1, D0 2, D1 ,and ... and a primer set of a 5¢ EcoRI-attached primerdesigned for the defined N-terminal amino acid sequence of NLS and a 3¢ SalI-attached primer designed for 3¢-UTR. The amplified cDNAs were subcloned ... However, there is no clearevidence for the nucleocytoplasmic shuttling activity of NLS and the involvement of Trn-1 in the nuclear import. The C-terminal 21 amino acid sequence enco-ded by the hnRNP...
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Báo cáo khóa học: Two distinct heterodisulfide reductase-like enzymes in the sulfate-reducing archaeon Archaeoglobus profundus pptx
... Only the amino-terminal sequence of the AF1376 gene productFig. 2. Room temperature reduced/oxidized difference spectrum of the purified HmeCD from A. profundus. The spectrum of the reducedenzyme ... 1.5-mLcuvette to record the oxidized spectrum. Solid sodiumdithionite was then added (2–5 mg) and several successivespectra of the reduced pyridine hemochromes wererecorded.1108 G. J. Mander et al. ... weredetermined by Edman degradation (Table 1). Using thesesequences, the genome of A. fulgidus was searched for corresponding genes [13]. The amino-terminal sequence of the 53-kDa polypeptide shows 45% sequence...
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Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx
... cytosolic DA represents an additional risk of oxidative stress, as DA can auto-oxidize at the neutralpH of the cytosol and thereby form toxic DA-quinonespecies, superoxide radicals and hydrogen ... showed the presence of a strong broad band centered around75 kDa in the DAT-expressing cells that was absent in the mock-transfected cells. The broad band is probablydue to the presence of glycosylated ... eliminated the toxicityproduced by addition of DA (Fig. 5).DiscussionDegeneration of the dopaminergic neurons of the sub-stantia nigra pars compacta is responsible for the major motor symptoms of...
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Tài liệu Báo cáo khoa học: Mixed lineage leukemia: a structure–function perspective of the MLL1 protein ppt
... a1 and a3 of KIX (Fig. 2B) [33]. When the MLL1 TAD peptide is added to the binary complex, the TAD peptide adopts a helical conformation inwhich the conserved residues of MLL1 TAD (residues2845–2853) ... ternarycomplex, the histone H3 peptide binds in a deep chan-nel that divides a pair of acidic lobes, one of which iscomposed of residues from the SET-I region and the other of residues from the SET-C and ... transactivation domain is shown in blue (drawn from PDB code: 1sb0). Positions of E665 and E555 of the CBP–KIX domain, and residues K291 and R294 of the c-Myb transactivation domain are indicated. (C) The CBP–KIX:cMyb:MLL1...
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Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): evaluation of the biological function of growth-inhibitory factor in the injured and neurodegenerative brain pdf
... oxidative damage. Specificantioxidants include superoxide dismutase for superox-ide, catalase for hydrogen peroxide, and glutathioneperoxidase for hydrogen peroxide and lipid peroxide.In addition, ... is important to note that the synthesis of MT1 andMT2 in the injured or diseased brain is quite differentfrom the synthesis of GIF. While GIF synthesis is gen-erally considered to be reduced ... However, the levels of GIF are altered dramatically in the neurode-generative or traumatically injured brain. The best-characterized example of this is for AD. Indeed, manystudies have analysed the...
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Tài liệu Báo cáo khoa học: Compartmentalization and in vivo insulin-induced translocation of the insulin-signaling inhibitor Grb14 in rat liver pptx
... Grb10and Grb14 in the regulation of IR degradation [5].Additional studies are thus needed to determinewhether Grb14 is involved in insulin-induced endocy-tosis and ⁄ or degradation of the IR.In ... them.Structural studies of the kinase domain of the IR incomplex with the PIR–BPS of Grb14 and of the Grb14 SH2 domain have allowed us to propose amodel for the Grb14–IR interaction. The PIR–BPSbinds as ... was then added in the presence or absence of GST–Grb14 at the indicatedconcentration. After 30 min at 23 °C, RCAM-lysozyme(6 lm) was added and incubations were allowed to proceedfurther for...
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
... the Materials and methods. Completion of the hydrolysis wasconfirmed by the disappearance of the (Met1)-ONC(M23L) signal in the MALDI-TOF mass spectra 1 h after the addition of AAP. The pH was adjusted to ... under the conditions of this study.On the other hand, mass peaks corresponding to the removal of additional residues were not detected, indicatingthat the aminopeptidase action was limited ... fitted to a two- state thermodynamic model combined with sloping linearfunctions for the native and denatured states, as describedpreviously [19]. The free energy of thermal unfolding (DGU)was...
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Tài liệu Báo cáo khoa học: "Improving Automatic Speech Recognition for Lectures through Transformation-based Rules Learned from Minimal Data" ppt
... to eliminate the need for developmental data sets, the available data werepartitioned only into training and test sets, withone additional hour set aside for code developmentand debugging.It ... utterancesin the manual and ASR transcripts of trainingdata, and then extracting the mismatched wordsequences, anchored by matching words. The matching words serve as contexts for the rules’application. ... for the RT parameter could be tested and the optimal onechosen for the evaluation task. Since we used nodevelopment set, we tested two more values for the rule pruning threshold: RT = 5 and...
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Tài liệu Báo cáo khoa học: "Phrase-Based Backoff Models for Machine Translation of Highly Inflected Languages" docx
... haveused backoff models for combining in-domain and42out -of- domain data for the purpose of bootstrap-ping a part -of- speech tagger for Chinese, outper-forming standard methods such as EM.4 Backoff ... 2004) for phrase extraction and decoding. Word alignmentis performed in both directions using the IBM-4 model. Phrases are then extracted from the word alignments using the method described in(Och ... probability distributions. They specify underwhich conditions the more specific model is usedand when the model “backs off” to the more gen-eral distribution. Backoff models have been usedin a...
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Báo cáo khoa học: Physicochemical properties and distinct DNA binding capacity of the repressor of temperate Staphylococcus aureus phage /11 doc
... at the middle of the molecule. Tryptic digestion of CI at the above two regions yielded two extremelyfolded structures or domains [designated N-terminaldomain (NTD) and C-terminal domain (CTD)] ... resi-dues involved in the dimerization of LexA CTD werealso present in the CTD of /11 CI (data not shown). The CTDs of two /11 CI monomers may therefore beresponsible for the formation of a dimeric ... are denoted by vertical arrowheads and stars, respectively. The start codons of CI and Cro are indicated by angledarrows. The first base of the start codon of Cro was considered as +1 and the...
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