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Báo cáo khoa học: Origin and properties of cytoplasmic and mitochondrial isoforms of taurocyamine kinase pptx

Báo cáo khoa học: Origin and properties of cytoplasmic and mitochondrial isoforms of taurocyamine kinase pptx

Báo cáo khoa học: Origin and properties of cytoplasmic and mitochondrial isoforms of taurocyamine kinase pptx

... 3381–3385. Cytoplasmic and mitochondrial taurocyamine kinases K. Uda et al.3530 FEBS Journal 272 (2005) 3521–3530 ª 2005 FEBS Origin and properties of cytoplasmic and mitochondrial isoforms of taurocyamine ... correlations of this relaxed sub-strate specificity and the possibility of cytoplasmic and mitochondrial isoforms of TK, we have amplified twocDNAs coding for Arenicola brasiliensis TKs and cloned ... activity for taurocyamine, lombricine (30% of that of taurocyam-ine) and glycocyamine (7% of that of taurocyamine) . Neither TK catalyzedthe phosphorylation of creatine. Comparison of the deduced...
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Báo cáo khoa học: Ion-binding properties of Calnuc, Ca2+ versus Mg2+ – Calnuc adopts additional and unusual Ca2+-binding sites upon interaction with G-protein pdf

Báo cáo khoa học: Ion-binding properties of Calnuc, Ca2+ versus Mg2+ – Calnuc adopts additional and unusual Ca2+-binding sites upon interaction with G-protein pdf

... Although de Alba and Tjandra have reported theaffinities (Kd of 47 and 40 lm)ofCa2+for peptidescomprising the EF-hands of Calnuc [42], there are noreports of the affinity of the metal ion ... b-band and thec-band upon the addition of Ca2+to the dye com-plexes of calmodulin and troponin C, with parvalbu-min, the J-band is simply lost and the bound dye isreleased. In the case of ... and d-crystallin behave similarly (showonly one band), whereas calmodulin and troponinbehave like each other. On the other hand, Calnuc and parvalbumin generate both the J-band and the c-band,and...
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Báo cáo khoa học: Dissociation/association properties of a dodecameric cyclomaltodextrinase Effects of pH and salt concentration on the oligomeric state pot

Báo cáo khoa học: Dissociation/association properties of a dodecameric cyclomaltodextrinase Effects of pH and salt concentration on the oligomeric state pot

... at low and high pHs and in the presence of KCl.Considering also the 3D structure of CDase I-5, theanalysis of the quaternary state of CDase I-5 revealedFig. 8. Fluorescence spectra of CDase ... Dimerization and oligomerization can pro-vide enzymes with a number of functional advantagessuch as high stability and control over accessibility and specificity of active sites [1,2]. An example of this ... FEBSconcentration, the effect of the dimer/dodecamer equi-librium on the enzymatic properties of CDase I-5 wasexamined at various concentrations of KCl. First, therole of KCl in dissociation of CDase I-5 was...
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Báo cáo khoa học:

Báo cáo khoa học: "Exploiting Aggregate Properties of Bilingual Dictionaries For Distinguishing Senses of English Words and Inducing English Sense Clusters" ppt

... Aggregate Properties of Bilingual Dictionaries For DistinguishingSenses of English Words and Inducing English Sense ClustersCharles SCHAFER and David YAROWSKYDepartment of Computer Science and Center ... con-sisted of a list of pairs of the form (foreign word, Englishword). Because bilingual dictionary structure varieswidely, and even the availability and compatibility of part -of- speech tags ... illustrates the kind of supportthe aggregate bilingual dictionary provides for partitioning the mean-ings of fair into distinct senses: blond and just.erage of 26 and a median of 3 foreign entries...
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Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx

Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx

... family of cytoplasmic enzymes that are important for several aspects of leu-kocyte biology. Both loss- and gain -of- function muta-tions in humans have been instrumental in ourunderstanding of their ... TEC,BMX [also known as epithelial and endothelial tyro-sine kinase (ETK)] and TXK [also known as restinglymphocyte kinase (RLK)]. The phenotypes of loss -of- function mutations in mammals mainly ... ancient and constitutes the second largest family of cyto-plasmic tyrosine kinases. In 1993, loss -of- function mutations in the BTKgene were reported as the cause of X-linked agammaglobulinemia. Of...
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Tài liệu Báo cáo khoa học: The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid ppt

Tài liệu Báo cáo khoa học: The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid ppt

... Bax and Bak on mitochondrial membranes, which liberatescytochrome c and activates caspase 3 and the apopto-some [23]. Transfection of tBid directly triggers mitochondrial- dependent apoptosis and ... anti-FLAG and blotted with anti-GFPto reveal Bid and tBid coimmunoprecipitation. (B) 293T cells were cotransfected with constant amounts of rLuc–Itch and various amounts of either Bid–GFP of tBid–GFP. ... activation of afamily of cytosolic cysteine proteases, caspases, whichare required for many of the morphological changesthat occur during apoptosis. The mitochondrial release of cytochrome c and...
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Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

... because they arehydrophobic and negatively charged. A comparison of rhodamine uptake, in the presence and the absence of uncouplers, allows the estimation of the effect of intracellular binding ... volume of 2.5 l3[49]. Data represent the mean ± SD of four independent experiments.Fig. 4. The effect of MK571 and NBD-Cl on TMRM distribution in K562 and 2008 cell lines. K562 cells and 2008 ... continuously with an excitation wavelength of 555 nm and an emission wavelength of 575 nm. Inhibition of the NBD-Cl-sensitive efflux allowed the uptake of TMRM and its quenching within the cells. Right:...
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Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf

Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf

... E22K, E22Q and D23N), and the availability of a rapid and simpleexpression and purification protocol will facilitatelarge-scale investigations of the molecular determinants of aggregation and fibrillation. ... suppliedwith the enzyme, and contained Aba, Abb and Abcat40 nm each, and the start and stop primers Abstart and Abstop at 600 nm each, and 200 lm each of dATP, dCTP,dGTP and dTTP. The product ... understanding the structural and biological properties of this molecule [13–16]. Using chemicallysynthesized Ab peptides, substantial progress has beenmade in understanding of the aggregation and...
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Tài liệu Báo cáo khoa học: Peptides corresponding to helices 5 and 6 of Bax can independently form large lipid pores pdf

Tài liệu Báo cáo khoa học: Peptides corresponding to helices 5 and 6 of Bax can independently form large lipid pores pdf

... and NMRstructure of human Bcl-xL, an inhibitor of programmedcell death. Nature 381, 335–341.25 Suzuki M, Youle RJ & Tjandra N (2000) Structure of bax. Coregulation of dimer formation and ... the help of the CDPro softwarepackage, which contains three commonly used programs:selcon3, contin ⁄ ll and cdsstr [42,43]. This softwareallows the use of different reference sets of proteins, ... Salgado1,21 Department of Biochemistry and Molecular Biology, University of Valencia, Spain2 Institute of Molecular Science, University of Valencia, Spain3 ITC-CNR Institute of Biophysics, Trento,...
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Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc

Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc

... antiparallel b-sheet (strands bC–bG)facing the interior of the phage particle, with anN-terminal hairpin (strands bAandbB) and two a-helices(aAandaB) shielding most of the upper surface of theb-sheet ... emitted at wavenumbermi and the summation is carried out over the range of appreciable values of F. The values of center of spectralmass of tryptophan were converted into degree of denatur-ation/dissociation ... lgÆmL)1.Fig. 3. Dissociation and denaturation of wild-type (WT) and mutant MS2 particles. (A) Light scattering and spectral center of mass measurements of WT MS2 as a function of urea concentration....
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