... Superposition of the active-site
residues of the 20 conformers of the solu-
tion structure of HtA. Catalytic groups E66
and H113 are shown in red, and side chains
of other residues in their vicinity ... quality and precision of the 20 structures are
summarized in Table 1, and a superposition and general
view of the structures is shown in Fig. 2A,B. The me...
... at the N- and C-termini. A
comparison of the b-turn structure of cER with the similar
region in the crystal structure of rat and human CD2 was
carried out. In the rat CD2 crystal structure, the ... structure in solution and clo-
sely mimic the b-turn s tructure of the surface epitopes of the
CD2 protein. Docking studies of CD2 peptides and CD5...
... toxin structure and potential surface
The characteristic NOE connectivities, reported in Fig. 3A,
and the analysis of the / and w angle distribution allowed
the identification of the type and boundaries ... [47] of the triple-stranded antiparallel b-sheet was
Fig. 3. Summary of data used in the determination of the secondary structure of crotamine. (A) P...
... helix on the other face. It is thought
that the two inner strands of the b-sheet lie within the major
groove of the DNA and that a hydrophobic pocket formed
by the side chains of Y123 and I125 ... [15].
The solution structure of the MBD of rat (r)MeCP2 has
recently been determined [17]. The MBD adopts a wedge-
shaped structure, composed of an antiparal...
... with the crystal structure of human CNPase cata-
lytic domain (rmsd of 2.3 A
˚
over 205 residues).
Sequence alignment of the catalytic domain of
RICH and related proteins (Fig. 1B) identifies the ... close to its
Fig. 2. Structure of goldfish RICH catalytic domain. (A) Stereo view of the backbone superposition of 10 lowest energy structures of the
RICH c...
... obesity.
Experimental procedures
Generation of H-PGDS TG mice
The coding region of human H-PGDS was cloned into the
downstream sites of the chicken b-actin promoter and the
CMV enhancer of the pCAGGS expression vector ... without fat, of WT and
H-PGDS TG mice (n = 6). Continuous
dissections of mouse fat and bone in the
whole body were quantified by use of a
mi...
... developing and evaluating the use of a
computer as a direct aid to facilitating human communi-
cation. The basic idea is to use the processing and
logical capabilities of the computer to aid in the ... lines of
text and the average comment or page is about 20 lines
of text.
EIES offers the user a complete set of differing inter-
faces including menus, com...
... metal-binding site
at the interface of the two domains, which consists
of four side chains: two (His31 and His79) from the
N-terminal domain and two (Asp165 and His169) from
the C-terminal domain (Fig. ... Mn-reconstituted
ApeSOD, the metal ion was five-coordinate in trigonal
bipyramidal geometry (Fig. 3A). Three of the ligands,
OD2 of Asp165, NE2 of His79 and...
... proportional to
the inverse of the average of the distance to the sixth
power (<r
)6
>), between the unpaired electron of the
paramagnetic agent and the interacting nucleus. Thus,
these agents ... along the horizontal axis was
used to convert the left structure to the right structure. The N-ter-
minus is at the left for every structure. The best-fit...
... 5 and 6)
and assignment of all signals (Table 2) revealed that the
carbohydrate structure of the major component of C. tra-
chomatis E LPS consisted of three Kdo- and two GlcpN-
residues. The
1
H-NMR ... have compared
the endotoxic activities of three chlamydial LPS of known
structure and defined purity. Whereas the chemical struc-
tures of the LPS from C....