Báo cáo khoa học: Secondary transporters of the 2HCT family contain two homologous domains with inverted membrane topology and trans re-entrant loops docx

Báo cáo khoa học: Fluorescence studies of the replication initiator protein RepA in complex with operator and iteron sequences and free in solution pdf

... free and bound unlabeled C160–RepA and wild-type RepA, with the protein concentration con-stant. The ratio of the apparent FW94 of bound and freeRepA was then used to estimate the fraction of ... on the orientation of the AEDANS absorp-tion and emission transition dipoles with respect to the protein axes. For a case where any of these are parallel to the long axis, the value takes the ... conﬁrm the presence of discretecomplexes under the conditions of the experiment, and that binding is complete, in agreement with the binding curves (Fig. 3), except for the case of the complex with...

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... metal-binding siteat the interface of the two domains, which consists of four side chains: two (His31 and His79) from the N-terminal domain and two (Asp165 and His169) from the C-terminal domain ... ligands in the Mn-reconstituted enzyme and an additional water oxy-gen, which, together with the OD2 of Asp165, the NE2 of His79 and the NE2 of His169, formed anequatorial plane (Fig. 3B). The metal ... residuesinvolved in the binding of the metal cofactor.ApeSODs in different forms were superimposed with the least square ﬁt of Ca atoms of the residues in the range10–200. ApeSOD and P. shermanii...

Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf

... AK, the N-terminalregions of the a-subunits serves as catalytic domains, and the C-terminal regions of the a-subunits and the b-subunits act as regulatory domains [10,11]. The regulatory domains ... according to the procheckprogram [21] is of good quality, with 95.4% of the res-idues in the most favored regions and 4.6% in allowedregions of the Ramachandran plot. The crystal structure of the Thr-free ... (A) Elution proﬁles of TtAKb in the presence and absence of 5mM Thr. The solid line with circles and the dotted line with squares indicate proﬁles in the presence and absence of 5 mM Thr,respectively....

Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx

... the cured Oc cells. The Oc cellswere cotransfected with the myc-Cardif (wild-type or mutantC508A) and NS3-4A expression vectors. The production of the myc-Cardif and NS3 in these cells was analyzed ... in Fig. 6C. The black and white arrowheads indicateCardif and the cleaved Cardif, respectively. (C) TRIF is not cleavedin the O cells. The O and Oc cells were transfected with the myc-TRIF ... that both the phosphorylation and dimerization of IRF-3 werealmost completely abrogated in the cells transfected with TLR3 or TRIF siRNA, but not in those trans- fected with the GL2 and TLR4...

Tài liệu Báo cáo khoa học: Kinetic characterization of the ﬁrst step of the ribozyme-catalyzed trans excision-splicing reaction docx

... the integrity of the bound species during gel electrophoresis, the gel and the running buffer were made of H10Mg buffer and were prewarmed to 44 °C before the samples wereloaded. The bound and ... polyacrylamide gel with the 5¢-end labeled substrate and 166 nM rPC ribozyme. The positions of the substrate and the substrate-cleavage product on the gel arelabeled. The lane marked (+) buffer contains ... is slower than the rate of cleavage.Lastly, the results indicate that a conformationalchange exists between the two steps of the TES reac-tion. Taken together, these results further demonstratehow...

Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

... from the reﬁnement to calculate the Rfree. The ﬁnal R and Rfreevalues of the model are 0.21 and 0.22 for the complete dataset up to 1.6 A˚. The electron density of the whole mainchain of the ... revealsthat the most similar regions of these proteinases medi-ate interaction of the two b-barrels, formation of the catalytic machinery and structures required for binding of the main chain of the ... in Fig. 2. To the east of the active site the 37- and 60 -loops border the S2¢ pocket of the proteinase. The observed differ-ences in the 37-loop result from interactions between the differing...

Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... accessibility and determines the shape of the active site entry. (e) The structure of the active site isessentially identical with the active sites of the MshB and LpxC proteins. The conservation of catalyticallyimportant ... zinc with the Ndatom of His12, one of the Odatoms of Asp15 and the Neatom of His113.His113 protrudes from helix a4 whereas His12 and Asp15 both belong to the loop which joins the b1-strand ... gray indicates the C-terminal part. The dimer’s formation is established by the incorporation of the b8-strand of one monomer into a b-sheet of the other monomer. The position of the zinc ion is...

Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... when the temperature is raised. The effect of DCMU on the traces was to increase the amplitude of step J with the concurrent decline of stepI, and to retard the rise to Fm. With dPQ, the J ... depends on the relative amplitude and rate constant of the J–Iphase compared to the amplitudes and rate constants of the two other phases. The above should be gov-erned by the balance between the ... amplitude and half-time of the J–I phase were bothdecreased with DCMU, demonstrating that the J–I riseis not directly related to the reduction of the PQ pool.A further analysis of the inﬂuence of...

Tài liệu Báo cáo khoa học: An investigation of the substrate speciﬁcity of the xyloglucanase Cel74A from Hypocrea jecorina pdf

... analysis of XXXGXXXG, before (A) and after (B) incubation with Cel74A, and the proposed cleavage pattern (C) with the theoretical m ⁄ z value (z in superscript) of the most important fragments (the ... consist of two b-propeller domains [14]. Sucha fold has been recognized in a wide range of proteins,including other GHs (clans E, F and J) [3] and hemo-pexin [20]. The CD spectrum of the latter ... map and, presumably, contain the sites of O-glycosylation,but we have not identiﬁed the speciﬁc site(s) or the form of the O-glycans. The CD spectrum of Cel74A looks a lot like that of an unordered...

Tài liệu Báo cáo khóa học: Numerical calculations of the pH of maximal protein stability pptx

... broad range of pHs to becompared. The origin of the optimum pH. The position of the optimum pH depends on the amino acid composition and on the organization of the amino acids within the 3Dstructure. ... is the pH of the solution and N is the number of ionizable residues. Zunfis the partition function of unfolded state and DGunfis the freeenergy of unfolded state. The reference state of ... independent of the position of all other residues in the protein, and the pairwise Lennard–Jones interactions, both with por-tions of the protein that are held rigid, and with conformers of side...

Xem thêm