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Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot

Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot

Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot

... Calcium and peptide binding to folded and unfolded conformations of cardiac troponin C. Electro-spray ionization and Fourier transform ion cyclotron resonance mass spectrometry. Eur J Mass Spectrom ... al. Thioxylo-oligosaccharide binding to xylanases FEBS Journal 272 (2005) 2317–2333 ª 2005 FEBS 2333 Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier ... using electrospray ionization Fourier transform ion cyclotron resonance (FT-ICR) MS and X-ray crys-tallography. Ultra-high mass- resolving power and mass accuracy inherent to FT-ICR allowed mass...
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Báo cáo khoa học: Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1 doc

Báo cáo khoa học: Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1 doc

... theabsence of cofactor reduction.Stopped-flow kinetic studiesReduction of the FAD/NADPH domain of NR1 wasinvestigated by stopped-flow methods using a photodiodearray detector. Aggregation of the ... units. To enable accurate determination of the FAD potentials,titrations of the NR1 FAD/NADPH domain were per-formed on samples of identical concentration over smallranges (100–150 mV) of the potential ... conditions.Reduction of the FAD/NADPH domain by NADPHwas also analysed using fluorescence detection. Enzymeconcentration was 4 lM and oxidation of NADPH wasmonitored by fluorescence emission...
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Báo cáo khoa học: Characterization of heme-binding properties of Paracoccus denitrificans Surf1 proteins doc

Báo cáo khoa học: Characterization of heme-binding properties of Paracoccus denitrificans Surf1 proteins doc

... to water and couples the free energy of this reaction to the generation of a transmembrane proton gradient.The mitochondrial enzyme consists of up to 13 subun-its, of which only the key subunits ... group of heme a, and most likely presents thecofactor in a spatial orientation suitable for optimal transfer to its targetsite within subunit I of cytochrome c oxidase.AbbreviationCOX, cytochrome ... heme a binding in Surf1FEBS Journal 278 (2 011) 1769–1778 ª 2 011 The Authors Journal compilation ª 2 011 FEBS 1773the loss of heme a in CtaA or the gain of the cofactorby Surf1. After separation...
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Báo cáo khoa học: Analysis of DNA-binding sites on Mhr1, a yeast mitochondrial ATP-independent homologous pairing protein potx

Báo cáo khoa học: Analysis of DNA-binding sites on Mhr1, a yeast mitochondrial ATP-independent homologous pairing protein potx

... concen-tration was determined using an e260 nmvalue of 7.0 · 103m)1Æcm)1.Expression and purification of Mhr1For the expression of recombinant Mhr1, E. coli BL21(DE3)pLysS DrecA cells were transformed ... recombina-tion of mtDNA in Saccharomyces cerevisiae. Themhr1-1 mutation causes defects in mtDNA duplication,partitioning to bud, and recovery of homoplasmy, all of which are attributed to the MHR1-dependent ... to determine howeach HP protein causes a similar structural change inthe DNA. These investigations should include theidentification and characterization of the DNA -binding regions and the binding...
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Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot

Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot

... FEBSCharacterization of HbpR binding by site-directedmutagenesis of its DNA -binding site and by deletion of the effector domainDavid Tropel1* and Jan R. van der Meer1,21 Process of Environmental ... the HbpR–DNA binding interactions were investigatedby site-directed mutagenesis of the operator region and by DNA -binding assays using purified HbpR. Mutations that disrupted the twofold sym-metry ... operator types 2, 4, 5, 7, 72 and 45 (Fig. 4).Interestingly, this showed that a loss of binding affinitycorrelated to a deterioration of binding contacts inonly those regions with new mutations,...
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Báo cáo khoa học: Determination of the reopening temperature of a DNA hairpin structure in vitro pptx

Báo cáo khoa học: Determination of the reopening temperature of a DNA hairpin structure in vitro pptx

... denaturation and renatur-ation manipulations, the fast annealing (fast renaturation) and slow annealing (slow renaturation) procedures(Materials and methods) were performed after the primer2 and ... primer 2 and primer 3 under fast and slow annealing conditions. (A) Primer extension with primer 2under slow and fast annealing conditions: lanes 1 and 2, primerextension product and primer-free ... These nucleic acid manipulations areclosely related to molecular hybridization of DNA or RNAmolecules, or DNA replication, reverse transcription and RNA transcription, during which DNA or RNA...
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Báo cáo khoa học:

Báo cáo khoa học: " Determination of Syntactic Functions in Estonian Constraint Grammar " docx

... of syntactic analysis of Estonian using Constraint Grammar. Constraint Gram- mar framework divides parsing into two different modules: morphological disam- biguation and determination of ... The process of syntactic analysis consists of three stages: mor- phological disambiguation, identification of clause boundaries, and identification of syntactic func- tions of words. This ... Analysis of Estonian The Estonian language is a Finno-Ugric language and has got a rich structure of declensional and conjugational forms. The order of sentence con- stituents in Estonian is...
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Báo cáo khóa học: Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo pot

Báo cáo khóa học: Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo pot

... humanglioblastoma cell migration into the brain through modifications to the actin cytoskeleton and levels of expression of smallGTPases. J. Neuropathol. Exp. Neurol. 61, 585–596.134 S. Andre´et ... ligand properties of theneoglycoproteins, we determined their binding to cells and to tissue sections as well as their biodistribution in mice afterinjection of radioiodinated material. Binding ... FEBS 2003 Determination of modulation of ligand properties of syntheticcomplex-type biantennary N-glycans by introduction of bisectingGlcNAcin silico,in vitro and in vivoSabine Andre´1,...
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Báo cáo khoa học: Determination of the metal ion dependence and substrate specificity of a hydratase involved in the degradation pathway of biphenyl/chlorobiphenyl pot

Báo cáo khoa học: Determination of the metal ion dependence and substrate specificity of a hydratase involved in the degradation pathway of biphenyl/chlorobiphenyl pot

... substrate binding site and the role of the metal cofactor in theenzyme.ResultsCloning, expression and purification of BphHThe bphH gene was inserted into the expression vectorspT7-7 [11] , pEMBL18 ... values of 6.0 and 8.0 for catalysis.Number of metal ion bound per enzyme and determination of coordination numberThe equilibrium concentration of Mg2+bound to BphH was determined by atomic ... include the activation of water forthe hydration reaction and ⁄ or the stabilization of theanion transition state. In the latter case, if the sub-strate coordination to the metal ion is not bidentate,interaction...
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Tài liệu Báo cáo khoa học: Bioinformatics of the glycoside hydrolase family 57 and identification of catalytic residues in amylopullulanase from Thermococcus hydrothermalis doc

Tài liệu Báo cáo khoa học: Bioinformatics of the glycoside hydrolase family 57 and identification of catalytic residues in amylopullulanase from Thermococcus hydrothermalis doc

... addition of 975 lLofNa2CO3(50 mM). Oneunit (U) of activity was defined a s the quantity of enzymenecessary to release 1 lmol of 2-chloro-4-nitrophenol permin under the assay conditions, using ... concerning theconservation of the catalytic machinery [8].Evolutionary relationshipsIn order to draw the present-day evolutionary picture of the family GH-57, several evolutionary trees were constructed.Figure ... AL662. The analysis of this sequence revealed that the encoded enzyme i s amember of GH-57 [21,22]. The cloning and expression of apuin Escherichia c oli has led to the production of a C-terminallytruncated...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018đề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDETrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Nghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếThơ nôm tứ tuyệt trào phúng hồ xuân hươngSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)Đổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ