... binding studies with purified recombinant
proteins ferredoxin reductase, ferredoxin and cytochrome
P450 comprising the morpholine mono-oxygenase from
Mycobacterium sp. strain HE5
Bernhard Sielaff and ... redox partners
from different sources [5,9,17–19]. The expression and
purification of the ferredoxin reductase FdR
mor
, the
ferredoxin Fd
mo...
... 0.01 lm,
and that of the D70G mutant was 0.196 ± 0.02 lm.
Effects of ligands on steady-state kinetics
The effects of the ligands (L: tyramine, serotonin and ben-
zalkonium) on the AAA and esterase ... of the wild-type enzyme:
7.3-fold in the presence of o-NPA, 5.2-fold with ASCh,
three-fold with ATMA and unaffected with o-NAC.
The actual binding site of serotonin...
... minor)
role in the hydrolysis of oligomeric substrates. The
SBS is located too far from the active site to influence
the binding and catalysis of these substrates in the
active site. The same statement ... that these
SBS residues are important for the activity on starch
and that they play a role in the binding of the enzyme
to bacteria of the oral cavity [15]. Nie...
... during
the 300 min incubation, and the extents of labelling
were quantified in comparison with that found with
cysteine-less ABCB1 and the G324C isoform. The
G324C mutant was assigned as the positive ... with
the hydrophobic (CM) and zwitterionic (BM) probes,
on the basis of the extent and rapid half-life of the
interactions. In contrast, the hydrophilic FM di...
... the early S phase with a
double thymidine block. We released cells from the
block and fixed them at the time points indicated in
Fig. 5C, and then analyzed the DNA contents of the
cells by flow ... cells
(Fig. S1). On the other hand, the northern blotting and
quantitative real-time PCR analysis also showed that
the expression of differentiation marker genes such as
fgf...
... were
stable to further proteolytic attack. From the N-termi-
nal sequence and the mass of the fragments, and from
the kinetics of the process (Figs S7–S9 and Table S5)
we concluded that the main sites ... from the
atomic coordinates of the discriminating Tt-GluRS (PDB
files: 1n75 for the complex with ATP; 1j09 for the complex
with ATP and Glu; 1n77 for th...
... concentration of 2 mm) and
the kinetic parameters of recombinant EhMGL1 and
EhMGL2 (Tables 1 and 2). Despite the heterogeneity
of the EhMGL1 preparation used in the previous
study [13], the kinetic constants ... of the BamHI site in the vector and
the XbaI site in the EhMGL1 gene, the product of the nes-
ted PCR was replaced with the corresponding regio...
... Indeed, the
results of these studies do suggest that the ACGME did not
go far enough. But how far is enough and at what point do
the increased errors associated with more frequent hand-offs
offset the ... http://ccforum.com/content/9/5/528
We agree with the recommendation that further research
should study the effects of sleep deprivation and work
schedule interventions...
... incubated with
the reactivase in the presence of ADP and Mg
2+
and
followed by nondenaturing PAGE, the bands of the
enzyme and the reactivase were markedly reduced
in density, and two bands of the enzymeÆreactivase
complexes ... analyses. Both band i and band vi of the
enzymeÆreactivase complexes comprised all of the
subunits from the enzyme (a, b, and c) an...
... and
28%, respectively.
Structure of the ERBB proteins and
diversity of their ligands
The EGFR gene is located on chromosome 7p12-13
and codes for a 170kDa receptor tyrosine kinase. All
ERBB proteins ... and ERBB4.
NRG1 and NRG2 bind to both ERBB3 and ERBB4,
whereas NRG3 and NRG4 only bind to ERBB4 [3].
Although these ligands show redundancy, heparin-
binding- EGF is th...