Báo cáo khoa học: Pulchellin, a highly toxic type 2 ribosome-inactivating protein from Abrus pulchellus Cloning, heterologous expression of A-chain and structural studies ppt
... expression of A- chain and structural studies
Andre
´
L. C. Silva
1
, Leandro S. Goto
1
, Anemari R. Dinarte
2
, Daiane Hansen
3
, Renato A. Moreira
4
,
Leila M. Beltramini
1
and Ana P. U. Arau
´
jo
1
1 ... silver-
stained. An apparent molecular mass of 59 kDa for
ABC
Fig. 2. (A) A- chain expression analysis in SDS ⁄ PAGE, 15%. Lane 1, molecular mass marker; lanes 2...
... 1944.1
a
1943.9 57– 72
D7 DDQVDAVTTLLNREYYN 24 02. 3
a
24 01.1 53– 72
D8 A GQVRMCEQN 22 89 .2
a
22 89.1 34– 52
B GQVRMCE 27 46.5
a
27 46.3 34–56
S1 QNLE NS (5 02. 2) 45–48
S2 VYTRDERRVE 1 321 .9
a
1 321 .7 22 –31
S3 ALHGQVYD ... 11
muecaloivmuiret
ca
bomo
rh
C
snacifir
t
inedsullicaboihT
e
sA
NR
enorepahc/esaetorP
DCP
esaPTG
nieto
r
PemeHneerG
e
sa
lor
dyH
esaremosiedif...
... potential calculated for Ca
2+
was
)17.3 mV and )26 .2 mV for 10 and 20 mm CaCl
2
,
respectively. A Ca
2+
to Na
+
permeability ratio
(P
Ca
⁄ P
Na
) of 5 was calculated from the changes in the
measured ... Electrical signals after amplification were
collected and digitized, at a sampling rate of 25 kHz, with
the aid of a computer equipped with an analogue-to-digital...
... view from bacterial genomics. Nat Prod Rep
24 , 1073–1109.
32 Umezawa H, Aoyagi T, Ogawa K, Obata T, Iinuma H,
Naganawa H, Hamada M & Takeuchi T (1985) For-
oxymithine, a new inhibitor of angiotensin-converting
enzyme, ... Batagov A, Ward J, Baganz F & Krabben
P (20 06) Identification of erythrobactin, a hydroxamate-
type siderophore produced by Saccharopolyspora eryth-...
... antisense AGCTGATCTTCGAAGATCTTCGAAGAT
Mutated HSE
sense
Biotin-TCGACTT
CAAGCTTGTACAAGCTTGTAG
Mutated HSE
antisense
AGCTGAAGTTCGAACATGTTCGAACATC
‘Scrambled’
oligonucleotide
Biotin-AACGACGGTCGCTCCGCCTGGCT
140
40
60
80
100
120
Counts ... temperature.
Assay procedure
The assay was run as a three-step assay: initial incubation of
the sample and probe, addition and incubation of the sam...
... Shigeno M, Nako K, Ichikawa T, Suzuki K, Kawakami
A, Abiru S, Miyazoe S, Nakagawa Y, Ishikawa H,
Hamasaki K et al. (20 03) Interferon -a sensitizes human
hepatoma cells to TRAIL-induced apoptosis ... predominantly expressed as two func-
tionally active isoforms, caspase-8 ⁄ a and caspase-8 ⁄ b.
J Biol Chem 27 2, 26 953 26 958.
25 Sohn D, Schulze-Osthoff K & Janicke RU (20 05) Cas...
... 5¢-GGGAATTCCATATGAGAGACAATA
TTTCCCGTTTATCAAATC-3¢ and 5¢-CCGCTCGAGT
CATTACTAGGTTCCCTGTCCAGTGTTACC-3¢ ,and
PDE1(Lys 321 –Thr 620 ) was amplified with primers 5¢-GGG
AATTCCATATGAAGAATGATCAATCTGGCTGCG
GCGCAC-3¢ and 5¢-CCGCTCGAGTCATTACTAGG
TTCCCTGTCCAGTGTTACC-3¢. ... African trypanosome
Trypanosoma brucei is the protozoon that causes the fatal
human sleeping sickness, as well as Nagana, a d...
... Bio-Imaging analyzer BAS -25 00 using
IMAGE GAUGE
V3.3 software.
Chromatin and protein DNA analysis
Micrococcal nuclease (MNase) digestion and in situ cleavage
by methidiumpropyl-EDTA–Fe(II) (MPE) was ... Biotechnology) and acetylated H3 (Upstate Bio-
technology), and antibodies against specific modifications
such as acetylated H3-K9 (Cell Signaling Technology) and
H3-K14 (Abcam) an...
... al.,
20 03b; Lee and Seneff, 20 08; Nagata et al., 20 04;
Nagata et al., 20 05; Nagata et al., 20 06; Tetreault et
al., 20 10b). This is one of the most active research
areas in natural language processing ... North
American Chapter of the ACL, pages 154–1 62.
Joel Tetreault, Elena Filatova, and Martin Chodorow.
20 1 0a. Rethinking grammatical error annotation and
evaluation...