... et al. Rainbow trout interleukin-11
FEBS Journal 272 (2005) 1136–1147 ª 2005 FEBS 1147
Cloning and expression of the first nonmammalian
interleukin-11 gene in rainbow trout Oncorhynchus mykiss
Tiehui ... (NQT) are in bold and underlined. The four potential poly(A) signals (AATAAA) in the 3¢-UTR and the TATA
box in the 5¢-flanking region are...
... acids
of the A-chain, the 19 amino acids linker, and 216 amino
acidsoftheB-chainoftheml gene was obtained. The
sequence was different from that obtained using degenerate
primers. The obtained ... (Met153 of the ML1p and ML2p B-chains and
Met156 of the ML3p B-chain corresponding to the ricin
B-chain Leu152; and Met234 of the ML1p and ML2p
B-chains correspond...
... calcium binding domains (EF1–4) and calcineurin A binding domain are shown in the box. The dot in the EF1 box repre-
sents the modified amino acids alanine (A) as compared to the oxygen containing-calcium ... calcium-
binding protein) [7,8]. CBL proteins contain four
Ca
2+
-binding EF hand motifs [9] and functions by
interacting and regulating a group of Ser ⁄ Thr pro-
te...
... DNA-binding domain of HSF, whereas clone
C2 contained an insert of 2771 nucleotides including introns
and an ORF encoding 513 amino acids.
By using 5¢ -and3 ¢-RACE, the full-length cDNAs of
clones ... with HindIII were used as
molecular markers and are indicated on the left.
Fig. 5. RT-PCR analysis of the HSF1a and HSF1b genes in rainbow
trout RTG-2 cells and tissu...
... bovine
D14-SR, regions of the deduced amino-acid sequence corresponding to the N-terminal and V8 peptide sequences determined in the sequencing
experiments of the protein puri®ed from b ovine ... our hypothesis, bovine cDNA encoding
the 38-kDa protein was cloned to iden tify the catalytic
activity of the expressed protein.
Cloning of the cDNA encoding bovine ster...
... promoters. Of these, amino acid residues 243
and 275 are involved in DNA binding and 244 and 246
are in very close proximity to DNA binding. This indi-
cates that p53R2 either does not use these residues ... towards
certain mutants. Of these, only residue 283 is involved in
DNA binding. However, residues 272 and 276 are close
to DNA binding. The other four residues are fur...
... lanes 2–4). The gel in Fig. 5A
shows many other proteins in each fraction, including
those rich in IRP1. To gain insight into the possibility
of coprecipitation of proteins with IRP1 and to assess
possible ... presequence in the latter case). This indicates
that aconitase activity is present in all strains, inde-
pendent of the origin of the cloned cDNA and whe...
... domains consist of a cen-
tral six-stranded parallel b-sheet flanked by a varying
number of a-helices. Residues involved in binding of
the cofactor ThDP are located at the C-terminal ends
of the ... suspend-
ing a comparatively mobile thiazolium ring between them. While the resi-
dues binding the two ends of ThDP are well conserved, the lining of the
active centre p...
... as synthe-
sis of the distribution of the consonants across the
languages.
In the current work, with the help of PlaNet we
provide a systematic study of certain interesting
features of the consonant ... these 21 consonants.
On the other hand Regime 2 of Figure 3(a) as well
as Regime 1 of Figure 3(b) comprises of the rest
of the consonants. The point mar...
... (1989) The limits of the
DNase I-sensitive domain of the human apolipoprotein
B gene coincide with the locations of chromosomal
anchorage loops and define the 5¢ and 3¢ boundaries of
the gene. ... or in
combination in lysines 5, 8, 12 and 16 in the gene for
histone H4 [125]. Of these, the only mutation that had
a specific effect on patterns of yeast...