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Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt

Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt

Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt

... mutants of cAMP receptor protein from Escherichia coli. J Biol Chem 278, 43020–43026.19 Błaszczyk U, Polit A, Guz A & Wasylewski Z (2002)Interaction of cAMP receptor protein from Escherichia coli ... 1001–1007.CRP conformational changes induced by DNA and cAMP M. Tworzydło et al.1116 FEBS Journal 272 (2005) 1103–1116 ª 2005 FEBS Fluorescence quenching and kinetic studies of conformational changes induced ... magnetic resonance study of the cyclicAMP receptor protein (CRP): assignments of the NHprotons of histidine and tryptophan residues and theeffect of binding of cAMP to CRP. Biochem J 107,304–309.14...
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Tài liệu Báo cáo khoa học: Multidentate pyridinones inhibit the metabolism of nontransferrin-bound iron by hepatocytes and hepatoma cells docx

Tài liệu Báo cáo khoa học: Multidentate pyridinones inhibit the metabolism of nontransferrin-bound iron by hepatocytes and hepatoma cells docx

... similarcharacteristics.Effect of chelators on uptake of NTBIThe effects of the chelators on NTBI uptake by hepatocytes and Q7 hepatoma cells were marked and similar. Allchelators decreased uptake to 20% or less of the ... contrast to NTBI uptake by Q7 cells, the uptake of Tf-bound Fe (TBI) by receptor- mediated endocytosis ismuch lower in hepatocytes in comparison with hepatomacells [23] and regulated by intracellular ... min) and almost totally block Fe uptake from citrate, with the same kinetics as DFP suggests thatTable 1. Effect of chelators on internalization of NTBI by normal and iron-loaded hepatocytes, and...
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Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf

Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf

... regulatory effects of miR-23a on theexpression of IL6R by RT-PCR and western blot, and found an inverse correlation between miR-23a and IL6R expression at both the mRNA and protein levels. To confirm ... miR-23a causes the downregulation of IL6R by binding to its first conserved binding site,which then leads to the development of gastric adeno-carcinoma.Materials and methodsHuman cancer tissue ... secondaryantibody. Protein expression was assessed by enhancedchemiluminescence and exposure to chemiluminescent film.LabWorksÔ Image Acquisition and Analysis Software wasused to quantify the band intensities....
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Tài liệu Báo cáo khoa học: Platelet factor 4 disrupts the intracellular signalling cascade induced by vascular endothelial growth factor by both KDR dependent and independent mechanisms ppt

Tài liệu Báo cáo khoa học: Platelet factor 4 disrupts the intracellular signalling cascade induced by vascular endothelial growth factor by both KDR dependent and independent mechanisms ppt

... VEGF121 -induced proliferation downstream from the receptor. Presumablythis inhibition occurs at/or upstream from Raf1 and downstream from PLCc. We found the chemokine receptor CXCR3B, a putative PF-4 receptor [34], in ... arget of thetyrosine kinase activity of the KDR and to be involved inVEGF -induced DNA synthesis [31]. P LCc phosphorylationwas induced by VEGF165(10 ngÆmL)1)andVEGF121(10 ngÆmL)1) and ... efficiently inhibits the binding of VEGF165 to its receptor, but not that of VEGF121[26]. Thus, PF-4 may d isrupt the KDR-mediatedsignal transduction induced by VEGF121 by means of anunknown m echanism...
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Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt

Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt

... and DNA binding to CRP (Eur. J. Biochem. 270) 1423Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein ... Ligand -induced conformational and structural dynamics changes in Escherichia coli cyclic AMP receptor protein. Bio-chemistry 41, 6660–6667.Ó FEBS 2003 Conformational changes induced by cAMP and ... 18.7 A˚on binding of cAMP to the anti -cAMP- binding sites of the protein. The distance between the sulfur atom of Cys178 and the C9–C10 bond of the indole ring of Trp85, derived from the crystal...
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Báo cáo khoa học: Comparing the substrate specificities of cytochrome c biogenesis Systems I and II docx

Báo cáo khoa học: Comparing the substrate specificities of cytochrome c biogenesis Systems I and II docx

... subdivided into proteins whichcontribute to the handling of heme and ligation of heme to the apocytochrome, and those involved in the provision of reductant to the apocytochrome in order to reduce ... addi-tion of reductant is a result of the reduction of a disul-fide in the apocytochrome.Production of endogenous E. coli cytochromes Escherichia coli contains a number of endogenousc-type cytochromes ... level of spontaneous heme attachment. Uncata-lyzed heme attachment to cytochrome c552is known to occur in the E. coli cytoplasm [9], and a small amount of cytoplasmic contamination of periplasmic...
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Báo cáo khoa học: Fluorescence studies of the replication initiator protein RepA in complex with operator and iteron sequences and free in solution pdf

Báo cáo khoa học: Fluorescence studies of the replication initiator protein RepA in complex with operator and iteron sequences and free in solution pdf

... interpretation of the origin of the static quenching. However, judging from the binding stoichi-ometry together with the shape of the binding curves(Fig. 3), it is safe to conclude that the quenching ... 5405inititator, upon binding to origin DNA. J Biol Chem278, 18606–18616.4 Giraldo R, Andreu JM & Dı´az-Orejas R (1998) Protein domains and conformational changes in the activation of RepA, a DNA replication ... Kru¨ger R, Ross W, Rakowski SA& Filutowicz M (2004) Binding modes of the initiator and inhibitor forms of the replication protein p to the cori iteron of plasmid R6K. J Biol Chem 279, 41058–41066.20...
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Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf

Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf

... growth factor activator inhibitortype 1 and activates pro-hepatocyte growth factorTomio Hashimoto1, Minoru Kato2, Takeshi Shimomura2 and Naomi Kitamura11 Department of Biological Sciences, ... hepatocyte growth factor activator inhibitor type 2; HGF, hepatocyte growth factor; HGFA, hepatocyte growth factoractivator; HPAI, highly pathogenic avian influenza; IC50,the concentration of ... activities of some members of the TTSP familyare regulated by endogenous protease inhibitors, whichinclude Kunitz-type inhibitors and serpins [2]. Hepato-cyte growth factor activator inhibitor type...
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Tài liệu Báo cáo khoa học: Diol dehydratase-reactivating factor is a reactivase – evidence for multiple turnovers and subunit swapping with diol dehydratase pdf

Tài liệu Báo cáo khoa học: Diol dehydratase-reactivating factor is a reactivase – evidence for multiple turnovers and subunit swapping with diol dehydratase pdf

... presence of ADP and Mg2+ and followed by nondenaturing PAGE, the bands of theenzyme and the reactivase were markedly reducedin density, and two bands of the enzymeÆreactivasecomplexes (upper and ... Positions of DD and its subun-its (aD, bD and cD) and the reactivase (DD-R) and its subunits (aR and bR) are indicated on the right of the gels. In (C), to improve visi-bility of the bands of ... c,e, and g). To determine their subunit compositions,bands i–vi were excised and subjected to SDS⁄ PAGEon 6% and 14% gels (Fig. 5B,C), followed by densito-metric analyses. Both band i and band...
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Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf

Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf

... transmembrane helix of subunit 8 and helices G and H1 of cytochrome b and between the N-terminus of subunit 8 and helix a of cytochrome b. The figure was constructed from the crystal structure of the yeast ... inner membrane of mitochondria [1,2].This respiratory enzyme catalyzes the transfer of electrons from ubiquinol to cytochrome c and couples the electrontransfer to vectorial proton translocation ... Assembly of the yeast cytochrome bc1complex (Eur. J. Biochem. 271) 1211transformation of Escherichia coli and isolation of plasmid DNA from bacterial cells were carried out by standardprocedures...
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