Báo cáo khoa học: Modulation of IMPDH2, survivin, topoisomerase I and vimentin increases sensitivity to methotrexate in HT29 human colon cancer cells docx

... type II is signiﬁcantly up-regulated in malig-nant cells [21,22]. Benzamide riboside, tiazofurine and mycophenolic acid are potent inhibitors of this enzy-mic activity and phase II ⁄ III clinical ... in the absence and in the presence of MTX. Targeting of vimentin was carried out by lipofecting aODN-VIM into paren-tal HT29 cells causing a decrease of 60% in theexpression of vimentin (Fig. ... associated with increasedexpression of vimentin [55], suggesting an inverse rela-tionship between vimentin and MUC1. Mimicking thedown-regulation of vimentin by aODN a decrease in MTX sensitivity...

Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc

... FEBS and ⁄ or its interaction with other proteins such as thePSI core.Incorporation of tagged PSI-G into PSIWhilst it is evident that both C-terminally and loop-tagged PSI-G can insert into ... PSI-G into Arabidopsis PSI complexes in vivo .In achieving this, we have been able to collect multiple lines of evidence that PSI-G inserts into the thylakoid mem-brane with a stromal loop and ... sought to determine the topology of PSI-G and to test the feasibility of rescuing Arabidopsis PSI com-plexes lacking PSI-G with tagged constructs. Successfulintroduction of a hexa-histidine (His)-...

Tài liệu Báo cáo khoa học: Modulation of a-synuclein aggregation by dopamine in the presence of MPTP and its metabolite pptx

... Machida Y, Miyazaki H, Mitsui K,Kuroiwa Y et al. (2008) RNA-binding protein TLS is amajor nuclear aggregate-interacting protein in hunting-tin exon 1 with expanded polyglutamine-expressing cells. ... metabolite of MPTP. This leads to an increase in the concentration of MPP+ in the dopa-minergic neurons, leading to selective damage to sub-stantia nigra, similar to idiopathic Parkinson’s disease.An ... transportdopamine and is toxic to dopaminergic neurons. Anumber of contradictory reports exist in the literatureregarding the role of MPTP and MPP+ in producingparkinsonism-like symptoms. It has...

Tài liệu Báo cáo khoa học: Modulation of F0F1-ATP synthase activity by cyclophilin D regulates matrix adenine nucleotide levels pptx

... intact mitochondria, it is anticipated that there maybe a signiﬁcant production of P i in the vicinity of theATPase within the matrix.CYPD ablation or its inhibition by cyclosporin A increases ... P i con-centration, which in turn could affect CYPD binding to F0F1-ATP synthase, and decreased binding of theinhibitory protein IF 1 to ATPase. IF1 is a naturallyoccurring protein that inhibits the ... rates in intact mitochondria. This is in good agreement with the predictions of the kineticmodeling, suggesting that a 30% increase in F0F1-ATPsynthase activity yields a 1.38–1.7% increase in...

Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

... plasmidcombinations were spotted either onto medium lacking histidine and leucine to select for the plasmids, or onto medium lacking histi-dine, leucine and uracil to monitor protein interactions. ... used in this work are listed in Table 5.Split-ubiquitin techniqueFor the split-ubiquitin interaction assays, 105wild-type cells carrying the split-ubiquitin plasmids were spotted ontoSC-His ... strain and the cla4D strainwas indistinguishable from that in the wild-type strain(Fig. 7A). Therefore, it seems likely that increased ste-rol biosynthesis in cla4D cells results in higher...

Tài liệu Báo cáo khoa học: Modulation of the enzymatic efﬁciency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site pptx

... tyrosine residues interact with each side of theisoalloxazine in plastidic FNRs. On the si-face of theflavin, which is buried within the protein structure, atyrosine aromatic side-chain (Y89 in ... mayconstrain the nicotinamide and or the terminal tyrosineagainst the flavin. It has been described that the bind-ing of NADH to lactate dehydrogenase conditions thenicotinamide glycosidic bond torsion ... Role of methionine56 in the control of the oxidation–reduction potentials of the Clostridium beijerinckii flavodoxin: effects of substitutions by aliphatic amino acids and evidence fora role of...

Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx

... quantities of proteinutilizing the principle of protein-dye binding. AnalBiochem 72, 248–254.26 Castellani O, Ribeiro LV & Fernandes JF (1967) Differ-entiation of Trypanosoma cruzi in culture. ... all our attempts to detect ODC or ADC enzy-matic activities in various strains of wild-type T. cruziepimastigotes, by adding radioactive ornithine orarginine to intact parasites or cell extracts, ... T. cruzi epi-mastigotes in synthetic media were supplemented withputrescine, together with cyclohexylamine (a knowninhibitor of putrescine conversion into spermidine)[15,16], the parasites...

Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf

... critical r ole in the regulation of cellproliferation, including SMC, by inhibiting signalling mol-ecules [ 45–47]. Caveolin-3 is the main isoform in muscle cells, including SMC [30]. Caveolin-1 ... in AA-induced proliferation using cells incubated w ith t heMAPK kinase (MEK) inhibitor, U0126. U0126 completelyinhibited the cyclin D1 promoter activity in AA-pretreatedSMC incubated with IL-1b and reduced ... alsotreated with the speciﬁc inhibitor, LY294002, to determinewhether PI3-K was activated in AA-mediated proliferation.This inhibitor blocked the a ctivation of the cyclin D1 genepromoter in AA-treated...

Báo cáo khoa học: Modulation of glucocorticoid receptor-interacting protein 1 (GRIP1) transactivation and co-activation activities through its C-terminal repression and self-association domains pptx

... CBP(cAMP-response-element-binding protein-bindingprotein) interacting sites in GRIP1 (glucocorticoidreceptor-interacting protein), and their importance forthe function of GRIP1. Biochem J 382, 111–119.27 ... 2183 Modulation of glucocorticoid receptor-interactingprotein 1 (GRIP1) transactivation and co-activationactivities through its C-terminal repression and self-association domainsPei-Yao Liu1, Tsai-Yuan ... co-activator in HeLa cells Because the C-terminal region of GRIP1 is involved in the repression of transactivation activity and self-association of GRIP1 (Figs 1–5), we examined therelationship...

Báo cáo khoa học: Modulation of nitric oxide-mediated metal release from metallothionein by the redox state of glutathione in vitro doc

... preference of zinc in the more flexible b-domain. In addition to the already described differences in flexibility of the b-domain in MT isoforms 1 and 2 [8], we found that thedomain specific distinctions ... that in vitro it suppresses the metal releasefrom metallothioneins. This points to an hitherto unknownmechanism or compound(s) being involved in this inter-action in living cells and information ... (2002)Kinetics of the anaerobis reaction of nitric oxide withcysteine, glutathione and cysteine-containing proteins: implica-tions for in vivo S-nitrosation. J. Chem. Soc. Perkin Trans. I. 2,663–669.55....

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