Báo cáo khoa học: Stimulation of p-nitrophenylphosphatase activity of Na+ ⁄ K+-ATPase by NaCl with oligomycin or ATP docx

... respect-ively. The phosphorylation reaction was started by theaddition of 32Pi with or without pNPP.The reaction mixture (0.5 mL) for phosphorylation by ATP was composed of 50 lgNa+ ⁄ K+-ATPase, 5 mmMgCl2,50mm ... the inhibitory effect of NaCl on pNPPase activity in the presence of NaCl up to 20 mm. In thepresence of NaCl at concentrations of more than20 mm, however, the pNPPase activity with oligomycin was ... of pNPPase by KCl inthe presence of NaCl with or without oligomycin. Inthe absence of oligomycin, increasing the NaCl con-centration decreased pNPPase activity (Fig. 2A). Inthe presence of...

Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by ﬂuorescence resonance energy transfer and the role of ATP hydrolysis pptx

... lM) and ATP or slowlyhydrolysable ATP analogues (2.0 mM). (1) Without OsDmc1 proteinin presence of ATP; (2) with OsDmc1 protein with ATP; (3) with OsDmc1 protein without ATP; (4) with OsDmc1 ... AMP-PNP; (3) with 1.25 lM of OsDmc1 in presence of 2.0 mM ATP- c-S; (4) with 1.25 lM of OsDmc1 in presence of 2.0 mM of ATP. C. Rajanikant et al. DNA strand exchange activity of OsDmc1 by FRETFEBS ... that of even minus nucleotide cofactor control. Notwithstanding the slow rates,the reactions with no nucleotide cofactor or with ATP- analogues did reachthe same steady state level as seen in ATP...

Báo cáo khoa học: Anti-HIV-1 activity of 3-deaza-adenosine analogs Inhibition of S-adenosylhomocysteine hydrolase and nucleotide congeners pot

... notimportant enzymes for the phosphorylation of DZNep or DZAri. To synthesize the nucleotides of DZNep or DZArifor direct testing on viral enzymes, the enzyme(s) responsiblefor phosphorylating ... decrease in the phosphory-lation of either [3H]DZNep or [3H]DZAri. Therefore, part of the mode of action of these analogs might be similar tothat of AZT, which is converted by cellular kinases ... atoms of NAD were restrained by harmonicforces and a force constant of 100 kcalÆmol)1ÆA˚)2.Toavoiduncontrolled global conformational changes of the protein,optimizations were performed...

Báo cáo khoa học: Proper targeting and activity of a nonfunctioning thyroid-stimulating hormone receptor (TSHr) combining an inactivating and activating TSHr mutation in one receptor pptx

... negativeconstraint, corresponds to the major unliganded form of thereceptor; the active conformation capable of promotingGDP/GTP exchange on the G-protein is achieved by afraction of the unliganded receptors ... cells with 200 lL NaCl/ Piplus 0.1% BSA. For the permeabilized cell assay,cells were ﬁxed with 2% NaCl/ Pi/paraformaldehyde (UCB,Brussels, Belgium) and then treated for 30 min with NaCl/ Piplus ... production with respect to WT-TSHr or T477Ialone, and a clear decrease with respect to P639S. Stimulation of IP accumulation by 100 mUÆmL)1 of bTSH exhibited a threefold stimulation of IP production...

Báo cáo khoa học: Construction and biological activity of a full-length molecular clone of human Torque teno virus (TTV) genotype 6 pptx

... digested with twoenzyme pairs (BamHI ⁄ SalIorXhoI ⁄ SalI), each pair cutting at bothsides of the linearization breakpoint. Southern detection was per-formed with a probe prepared by nick translation ... characterization of the origin of DNA replication of porcine circovirus. J Virol 71,2562–2566.34 Goryo M, Suwa T, Matsumoto S, Umemura T & Itak-ura C (1987) Serial propagation and puriﬁcation of chicken ... of the linearization breakpoint(Fig. 5). BamHI ⁄ SalIorXhoI ⁄ SalI double-digestions of the input linear construct yielded three restrictionfragments of approximately 2100, 740 and 890 bp with the...

Báo cáo khoa học: The potent inhibitory activity of histone H1.2 C-terminal fragments on furin doc

... fragments as inhibitors of furin will undoubtedly pave the way for the devel-opment of therapeutically useful furin inhibitors andfor the mechanistic studies of the regulation of furin activity in ... assayed for their inhibitory activity. Among them, the major fractions P2, P3 andP4 have a strong inhibitory activity against furin. Thehomogeneity of the three fractions was detected by SDS ⁄ PAGE, ... with 0.1% (v ⁄ v)triﬂuoroacetic acid, and the bound proteinswere eluted with a linear gradient of 0–20%(v ⁄ v) acetonitrile in 0.1% (v ⁄ v) triﬂuoroaceticacid in 0–20 min and 20–100% (v ⁄...

Báo cáo khoa học: Lpe10p modulates the activity of the Mrs2p-based yeast mitochondrial Mg2+channel pot

... absence of Lpe10p.The F ⁄ YGMN motif is essential for the Mg2+transport activity of Lpe10pThe conserved F ⁄ YGMN motif in TM1 of CorA-likeproteins cannot be varied without loss of Mg2+uptake[4,18]. ... lpe10D cells restoredDW close to WT levels, meaning that the loss of Fig. 1. Growth phenotypes of yeast strains with deleted MRS2and ⁄ or LPE10. Serial dilutions of DBY 747 mrs2D, DBY747 lpe10Dand ... Weanalysed the complementation of strains with deletedMRS2 and ⁄ or LPE10 by Mrs2p or Lpe10p expressedfrom episomal high copy number (H) or low copynumber (L) vectors (Fig. 1). In the cross-wise...

Báo cáo khoa học: Fibroblast growth-stimulating activity of S100A9 (MRP-14) ppt

... Science Laboratory 99 Image Gauge program(Fuji Photo Film). Chemiluminescence of the band linearlycorrelated with the amount of recombinant S100A9 (25 to200 ng per lane). For quantitation of S100A9 ... gradient of acetonitrile from 28 to40% (v/v) in 0.05% (v/v) triﬂuoroacetic acid, evaporated,dissolved in phosphate-buffered saline with 1 mMcalciumchloride and stored at – 20 °C. Oxidation of ... mediated by growth factors andcytokines, including platelet-derived growth factors [2],transforming growth factor b [3], ﬁbroblast growth factors[4–6], and connective tissue growth factor [7]....

Báo cáo khoa học: Plasminogen activator inhibitor type-1 inhibits insulin signaling by competing with avb3 integrin for vitronectin binding pptx

... growth factorreceptors has been reported for several systems, one of whichis the modulation of insulin signaling by avb3 integrin.Plasminogen activator inhibitor type-1 (PAI-1), competes with avb3 ... Inhibition of insulin/VN induced PKB phosphorylation by PAI-1.NIH3T3 cells were incubated overnight with 100 nMechistatin (Ec) or decorsin (Dc), or for 4 h with 30 nMVN, 100 nMPAI-1 or 200 ... Integrins can collaborate with growth factors for phos-phorylation of receptor tyrosine kinases and MAP kinase activa-tion: roles of integrin aggregation and occupancy of receptors.J. Cell Biol....

Tài liệu Báo cáo khoa học: Stimulation of poly(A) synthesis by Escherichia coli poly(A)polymerase I is correlated with Hfq binding to poly(A) tails ppt

... investigatedwhether stimulation of PAP I activity is correlated with the affinity of Hfq for the 3¢ end of RNA. Weused an RNA fragment corresponding to the 3¢ end of the rpsO transcript, which ... weremonitored by counting out the radioactivity. Labelling of the 5¢ end was performed with [32P ]ATP[ a˜P] and T4 poly-nucleotide kinase, labeled RNAs were separated from un-incorporated nucleotides ... downstream of the rpsO transcription termi-nator are either not, or only inefficiently, bound by Hfq. In order to determine which RNA substrate ismore efficiently bound by Hfq, we performed competi-tion...

Xem thêm