Báo cáo khoa học: Phaiodotoxin, a novel structural class of insect-toxin isolated from the venom of the Mexican scorpion Anuroctonus phaiodactylus pdf

... Phaiodotoxin, a novel structural class of insect-toxin isolated from the venom of the Mexican scorpion Anuroctonus phaiodactylus Norma A. Valdez-Cruz1, Cesar V. F. Batista1, Fernando ... tran-scriptase. The cDNA was joined with the a daptor providedby the kit (5 ¢-gcugauggcgaugaaugaacacugcguuugCUGGCUUUGAUGAAA-3¢) using T4 DNA ligase. The modi-fied cDNA was used as t emplate for PCR amplification.Two ... describe the isolation and characterization of aninsect specific toxin from the scorpion Anuroctonus phaiod-actylus, collected in Baja California, Mexico. We have isolated and chemically and functionally...

Tài liệu Báo cáo khoa học: TransLISA, a novel quantitative, nonradioactive assay for transcription factor DNA-binding analyses pdf

... procedure The assay was run as a three-step assay: initial incubation of the sample and probe, addition and incubation of the sampleand acceptor beads in the plate wells, and addition of donorbeads ... between the addition of reagents. The broad analytical range of the assay allows quanti-tation of large differences in the DNA-binding activity of transcription factors. This is precluded in EMSAanalysis, ... that the assay speciﬁcally measures HSF1DNA-binding activity.Analytical range and precision The analytical range of the assay was evaluated usingknown concentrations of recombinant human HSF1instead...

Tài liệu Báo cáo khóa học: TbPDE1, a novel class I phosphodiesterase of Trypanosoma brucei pdf

... 5¢-GGGAATTCCATATGAGAGACAATATTTCCCGTTTATCAAATC-3¢ and 5¢-CCGCTCGAGTCATTACTAGGTTCCCTGTCCAGTGTTACC-3¢,andPDE1(Lys321–Thr620) was amplified with primers 5¢-GGGAATTCCATATGAAGAATGATCAATCTGGCTGCGGCGCAC-3¢ and 5¢-CCGCTCGAGTCATTACTAGGTTCCCTGTCCAGTGTTACC-3¢. ... explore the PDEs of parasites as potentialtargets for antiparasitic drugs. The African trypanosomeTrypanosoma brucei is the protozoon that causes the fatalhuman sleeping sickness, as well as Nagana, ... N-terminally truncated fragments of TbPDE1were also amplified using the same protocol and pET-PDE1as template. PDE1(Arg189–Thr620) was amplified using the primer pairs 5¢-GGGAATTCCATATGAGAGACAATATTTCCCGTTTATCAAATC-3¢...

Báo cáo khoa học: How a lipid mediates tumour suppression Delivered on 29 June 2010 at the 35th FEBS Congress in Gothenburg, Sweden pdf

... a loss of both epithelial andplanar polarity [47,48]. The loss of epithelial polarity is a prevailing characteristic of carcinomas, and muta-tion of Rabenosyn indeed causes epithelial tumours ... landscapes of human breast andcolorectal cancers. Science 318, 1108–1113.87 Tsukazaki T, Chiang TA, Davison AF, Attisano L &Wrana JL (1998) SARA, a FYVE domain protein thatrecruits Smad2 to the ... of UVRAG in autophagy is supported by the independent identiﬁcation of BIF-1, an interactor of UVRAG, as a regulator of autophagy [5]. On the other hand, monoallelic UVRAG mutations associatedwith...

Báo cáo khoa học: Puriﬁcation and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot

... 27693QEGYGSTQLPSDEPLNGLNDCAA GAA GGA TAT GGC AGC ACC CAA CTT CCA TCG GAC GAG CCA TTG AAC GGG CTC AAC GAC 336113KAIQDILNEHNMFRAKERVPAAG GCC ATA CAG GAC ATC CTC AAC GAG CAC AAC ATG TTC CGC GCC AAG GAG CGC GTC CCG ... GAG CAG GTA GCG TCC GAG TCA GCC TCC CTC ACA ACA CCA 21673TTREEQPAAEAAASTAGGSQACC ACC AGG GAA GAA CAA CCG GCG GCA GAG GCC GCG GCG TCC ACC GCT GGC GGT AGC CAA 27693QEGYGSTQLPSDEPLNGLNDCAA GAA ... 516173SGAISWKTTVDTWSDEKKSYTCC GGC GCC ATC TCC TGG AAG ACG ACG GTG GAC ACG TGG AGC GAC GAG AAG AAG AGC TAC 576193HYGSNTCDQGKMCGHYTAVVCAC TAC GGC TCC AAC ACC TGC GAC CAA GGC AAG ATG TGC GGC CAC TAC...

Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... M, Teramoto T, Kumagaye KY, Nakajima K,Watanabe T, Kawai T, Kawakami Y, Niidome T,Sawada K, Nishizawa Y et al. (1994) Omega-agatoxin-TK containing D-serine at position 46, but not syn-thetic ... Kubota I, Takao T, Shimonishi Y,Yasuda-Kamatani Y, Minakata H, Nomoto K,Muneoka Y & Kobayashi M (1991) Fulicin, a novel neuropeptide containing a D-amino acid residue isolated from the ganglia ... China Sea. Sephadex G-25 was purchased from Amersham Biosciences (Uppsala, Sweden), a ZORBAX300SB-C18 semipreparative column was from Agilent Tech-nologies (Santa Clara, CA, USA), and triﬂuoroacetic...

Báo cáo khoa học: Pulchellin, a highly toxic type 2 ribosome-inactivating protein from Abrus pulchellus Cloning, heterologous expression of A-chain and structural studies ppt

... A pair of degenerate primers (abrin 1: 5¢-ACTGAAGGTGCCACTTCACAAAGCTAYAARCARTT-3¢; abrin 3: 5¢-GGTTAAACACTTCCCGTTGGACCTDATNGT-3¢) was cho-sen to represent the possible coding sequences of the ... obtained previously. Thus, the sequences of the primers used for 5¢ RACE were: 5¢-GGGCATCACGGAAGAAATAG-3¢ for a reverse transcription and 5¢-GCTCTAGAGCATTCGTCACATCGATACC-3¢ with 5¢-AAGGAATT(dC)14 ... expression of A- chain and structural studiesAndre´L. C. Silva1, Leandro S. Goto1, Anemari R. Dinarte2, Daiane Hansen3, Renato A. Moreira4,Leila M. Beltramini1and Ana P. U. Arau´jo11...

Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf

... Tsubouchi H, Naka D, Takahashi K,Okigaki M, Arakaki N, Nakayama H, Hirono S, Sakiy-ama O, Takahashi K et al. (1989) Molecular cloningand sequence analysis of cDNA for human hepatocytegrowth factor. ... Yokohama, Japan2 Advanced Medical Research Laboratory, Mitsubishi Tanabe Pharma Corporation, Kamoshida-cho, Aoba-ku, Yokohama, JapanIntroductionType II transmembrane serine proteases (TTSPs) arestructurally ... °C. Then each substrate was added to the mixture at a ﬁnal concentration of 100 lm. After incuba-tion for 3 h at 37 °C, the amount of 7-amino-4-methyl-coumarin liberated from the substrate was...

Tài liệu Báo cáo khoa học: Erythrochelin – a hydroxamate-type siderophore predicted from the genome of Saccharopolyspora erythraea docx

... Marburg, GermanyIntroductionBacterial growth is strongly inﬂuenced by the availabil-ity of iron as an essential trace element employed as a cofactor [1]. The fact that the bioavailability of ... residue pattern, leadingto the assumption that both activate the same sub-strate (Table S 2A) . On the basis of the bioinformaticanalysis of the etc gene cluster, it was predicted that the assembled ... on A- domain speciﬁcity prediction and the available tran-scriptome data, can be applied for the initial detectionand isolation of NRPs [20]. Furthermore, thisapproach substitutes the CAS assay-guided...

Tài liệu Báo cáo khoa học: S100–annexin complexes – structural insights pptx

... in calcium signalling.AcknowledgementsThis work was supported by a grant from the Cana-dian Institutes of Health Research (GSS) and anaward from the Canada Research Chairs program(GSS). A. ... truncated and mutant forms of the annexins (particularly annexin A5 ), as well asannexin–protein complexes. In particular, vertebratestructures of human annexins A1 , A2 , A3 , A5 , A8 andbovine annexins ... within each of the two core domains of annexin A6 [10]. A similar conclusion has beenreached for the interaction of S10 0A1 and S100B withannexin A6 [9]. In the latter case, and for the S10 0A1 2–annexin...

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