Báo cáo khoa học: Radical-induced oxidation of metformin pptx

Tài liệu Báo cáo khoa học: Critical roles of Asp40 at the haem proximal side of haem-regulated phosphodiesterase from Escherichia coli in redox potential, auto-oxidation and catalytic control doc

... containin g 1 mMEDTA. The auto -oxidation rates of Asp40Ala and Asp40Asn mutants were meas-ured at 25 °C. Oxidation of t he sample was observed b yFig. 1. Structure of ferric Ec DOS PAS (PDB code ... region of the Fe(III) species, spectra of the Asp40 mutants wereessentially similar to that of the wild-type protein.Additionally, spin states of the Fe(III), Fe(II) andFe(II)-CO complexes of ... structure of the cyanide-boundcomplex of FixL is similar to that of its oxygen-boundcomplex [10], a cyanide binding study should facilitateelucidation of the structure and catalytic mechanism of Ec...

Báo cáo khoa học: Thioredoxin Ch1 of Chlamydomonas reinhardtii displays an unusual resistance toward one-electron oxidation potx

... selective and provides a simpler model of oxidation than OHradicals allowing the determination of the mainprocess of o xidation of OH•radicals, without all of the side-effects. They are known ... formethionine. Any of these transformations may affect thefunction of the enzyme and thus the redox homeostasy.The aim of this work was to determine the sensitivity of Trx toward one-electron oxidation. ... one-electron oxidation of wild-type thio-redoxin and of two forms with the point mutations D30Aand W35A, using azide radicals generated by c-ray or pulseradiolysis. The oxidation patterns of wild-type...

Báo cáo khoa học: "Circadian pattern of activation of the medical emergency team in a teaching hospita"

... 08:00–18:00 (47% of calls during 42% of the day), asubstantial proportion of MET calls occur after normal workinghours (53% of calls during 58% of the day), with the peak time of activity occurring ... time, a detailed analysis of the level of utilization of a MET service over a 24-hour period and found asignificant increase in the number of MET calls around periods of nursing handover and routine ... implementation of a MET systemin our hospital resulted in a 65% relative risk reduction for in-hospital cardiac arrest over a 4-month period [4]. Analysis of the pattern of activation of the MET...

Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc

... derivative of glycine. GO catalyzes thedeaminative oxidation of glyphosate, yielding glyoxy-late, AMPA, and hydrogen peroxide, using 1 mol of dioxygen per 1 mol of herbicide (Fig. 3B). The efﬁcient oxidation ... however, was devoid of substrate or inhibitor,and consequently did not reveal the nature of theactive site or the mode of action of glyphosate. A dec-ade later, the crystal structure of EPSPS was ... 4% of thetotal soluble protein content of the cell [48].Fig. 3. Microbial mechanisms of glyphosatedegradation. (A) Two principal pathways of glyphosate degradation are known.Top: cleavage of...

Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt

... of tau with Fyn-SH3. We alsoshow that tau interacts with the SH2 domain of Fyn, and that this associa-tion, unlike that of Fyn-SH3, is inﬂuenced by Fyn-mediated tyrosine phos-phorylation of ... SH3 domain of Fyn binds to proline-richmotifs within the sequence PXXP in interacting pro-teins. Seven such motifs are present in the proline-richdomain of the longest isoform of tau in the ... this band was notapparent on western blots of lysates of Y394F tau(Fig. 2A). The absence of this larger species could indi-cate that phosphorylation of tau by Fyn is reducedand therefore, in...

Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

... diffusion method. Two microlitres of protein solution at a concentration of 9 mgÆmL)1and 2 lL of crystallization solution were equilibrated against 500 lL of reservoir solution. Initial screens ... reactions werestarted by addition of substrate, and the rate of substrate oxidation was measured by monitoring the change in absor-bance over 5 min. All of the measurements were performedin ... laccase-catalyzed oxidation of N-hydroxy compounds. Appl Environ Microbiol 66,2052–2056.31 Xu F, Deussen HW, Lopez B, Lam L & Li K (2001)Enzymatic and electrochemical oxidation of N-hydroxycompounds:...

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... reservoir consisted of 500 lL of 0.7 m sodium citrate,10 mm dithiothreitol and 70 mm Hepes at pH 7.4. Dropsconsisted of 0.75 l L of importin-a (70-529) at 18 mgÆmL)1,0.75 lL of CLIC4 NLS peptide ... Luria–Bertani medium containing30 lgÆmL)1 of kanamycin. Flasks of 400 mL of Luria–Bertani medium (30 lgÆmL)1kanamycin) were then inocu-lated with 10 mL of the overnight culture. The cells weregrown ... possibility of trigger events to expose NLSsoffers an interesting criterion for cargo selection bynuclear import receptors. Typically, NLSs are identiﬁ-able as a result of their high content of basic...

Tài liệu Báo cáo khoa học: PCR detection of nearly any dengue virus strain using a highly sensitive primer ‘cocktail’ ppt

... comparison.Effect of geographical variation in dengue viruson the performance of the primer cocktailEach of the four serotypes of dengue virus can be classi-fied into several genotypes, defined as a group of ... Yuriy Fofanov1,3and Richard C. Willson1,71 Department of Biology and Biochemistry, University of Houston, TX, USA2 Department of Chemical and Biomolecular Engineering, University of Houston, ... excess of contami-nating human DNA. Serotyping potentially could beachieved by electrophoretic analysis of hybrid products(Fig. 6A, B; all predicted products of amplification of each of the...

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... (Fig. 3A). Three of the ligands,OD2 of Asp165, NE2 of His79 and NE2 of His169,formed an equatorial plane. The other protein ligand,NE2 of His31, bound to the metal, in the company of a water oxygen, ... conformationaround the active site of ApeSOD is independent of the presence of a metal cofactor.Figure 3D shows the superimposition of the activesite structures of apo, Mn-bound and Fe-bound Ape-SODs. ... Japan3 Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Japan4 Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate...

Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... quantitation of microgram quantities of protein uti-lizing the principle of protein-dye binding. Anal Bio-chem 72, 248–254.48 Laemmli UK (1970) Cleavage of structural proteinsduring the assembly of ... report data on homologousexpression of the nrdF gene of C. ammoniagenes strainATCC 6872. This is the ﬁrst report of the successfulpuriﬁcation of high amounts of the native C. ammoni-agenes R2F ... time-scan (B) of a novel enzyme assay; continuous black line, course of enzyme reaction; arrow, time point of substrate addition; triangles, control(reaction by addition of BSA instead of substrate)....

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