Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
... Consequently, the molecularstudies of the interaction of SAgs with their s pecifics ligandswill not only advance understanding of the physiologicalmechanisms of these molecules, but may lead to the development ... bearing T-cell subsets are expanded by interaction with SSA. Someauthors indicated clonal expansion of T-cells bearing human Vb3, Vb12, Vb17 and Vb19 [20]. Others showedproliferation of human T-cells ... Fig. 1B–D, expression of the mutant yields only monomeric SSA, free of dimer. T-cell proliferation assayWe next analyzed the ability of recombinant SSA tostimulate human T-cells. All SSA preparations...
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Báo cáo khoa học: Cloning, expression and characterization of a family-74 xyloglucanase from Thermobifida fusca pptx
... glucose standard curve and the nmoles of XGOs produced was determined for both the PAHBAH and the bicinchoninic acid reducing sugarmethods. For the PAHBAH method, the nmol of XGOs ¼ nmol of glucose ... furtherwashing with 0.04% NaN3. Pieces of the gels were rinsed with water, blotted on filter paper and chopped finely with arazor blade. The dry mass of the material was approxi-mately 1% of ... cuts cleanly and Xeg74 does not,as both genes are cloned in the same way except for the presence of the linker and the family-2 CBM at the Cterminus of XEG74. The molecular masses of Cel9A-68(CD...
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Báo cáo khoa học: Cloning, expression and characterization of a new aspartate aminotransferase from Bacillus subtilis B3 docx
... 7.2, and the stability of the enzyme was examined by keeping the purepreparation for 5, 15 and 30 min at 40, 50, 60 and 65 °Cbefore the assay. The effect of inhibitors was established with the ... tostrengthen the electron-withdrawing capacity of the co-enzyme [15]. The active site Lys258 transfers aproton from the amino acid substrate to the cofactor and forms an internal Schiff base with the ... determine the exact role of three residues in AATB3. The loss of the activity from the mutations together with the multiplealignment analysis indicated that the Asp232 residue of AATB3 enhances the...
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Báo cáo khoa học: Structure, expression and regulation of the cannabinoid receptor gene (CB1 ) in Huntington’s disease transgenic mice ppt
... by the expression of mutant huntingtin and the exact mechanism by which mutant huntingtin alters the function of such factors. This work describing the genestructure of CB1 and i ts pattern of ... full complement of mouse huntingtin. Transgenic R 6 mouse models differ in the length of the CAG repeat within exon 1 of the human HD transgene and site of transgene integration. The R6/1model ... repeat and relative expression of the HD gene. W e then determined the structure of the mouse CB1 gene and determined thattranscription of t he CB1 gene was a ffected in striatalneurons of transgenic...
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Báo cáo khoa học: Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus potx
... 6); and #PFLF-1 and #PFLR-1 (lanes 7 and 8). (B) Schematic organization of the CrPrxgene. The asterisk indicates the position of the codon encoding the first amino acid of the mature protein, and ... during the first hour. In the case of UV and cold exposure, the maximum transcript level wasobserved at 12 and 24 h, respectively. On the otherhand, a gradual steady-state increase in the expression level ... cDNA, and lanes 1, 3, 5 and 7 show the same using genomic DNA of C. roseus. Primer pairs were:#GSP-4 and #PFLF1 (lanes 1 and 2); #GSP-2 and #GSP-4 (lanes 3 and 4); #GSP-2 and #PFLR-1 (lanes 5 and...
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Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes pot
... reading and help in preparation of this paper.REFERENCES1. Venulet, J. & Van-Etten, R.L. (1970) Biochemistry and pharma-cology of the nitro and nitroso group. In The Chemistry of the Nitro and ... pre-equilibrated with 10 mMsodium phosphate buffer (pH 8.0). The column was first washed with the same buffer containing0.2MNaCl, and then the proteins were eluted with a0.2–0.4MNaCl gradient in the ... coelicolor, 27% with 2-nitropropane dioxyg-enase of W.saturmusvar. mrakii, 26% with 2-nitropropanedioxygenase of N. crassa, and 36% with the putative2-nitropropane dioxygenase encoded by yrpB of Bacillussubtilis....
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Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
... products of b-actin. The x-axis indicates the time-periods of LPS incubation and the relative expression of the carp IL-10 gene on the y-axis. Controls for 0, 1, 3 and 6 h of incubation without ... isolated and its genomic structure and expression analysis investi-gated. This work will pave the way for further investigation of the biological function of this gene, and the probability of the ... their positions. The arrowheads depict the residues important for the structural core of the IL-10 gene. The underlined amino acid residues are the signal sequences of the respective genes. The...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
... incatalysis in the bacterial enzyme and the corresponding amino acids in the human protein are pointed with arrows. The meanhydrophobicity values of residues within the window centered on the pointed ... superimposing the ACMSDmodel with the bacterial ACMSD–Zn2+complex and past-ing the zinc ion in the predicted human structure. Figureswere generated with the software swiss-pdbviewer and visual molecular ... totake up the metal from the expressing host duringprotein synthesis [21,33], we investigated whether the production of the human recombinant isoforms mightbe enhanced by the presence of metal...
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Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx
... of Science, The Blix Family Foundation and grants from the University of Oslo and the NationalCancer Institute of the USA (R01-CA81150). The experiments were performed in accordance with all regu-lations ... increased the phosphorylation of the endogenous 140 kDa and 60 kDa isoforms of Ras-GRF1 (Fig. 2A, left panel) and of recombinant HA-Ras-GRF1 (Fig. 2B, upperpanel). Phosphorylation of ERK1 ⁄ 2 in the ... phosphorylation of both isoforms wasreduced by the coexpression of hPDE4D2 with 5-HT7 receptors (Fig. 3C). This was also the case for ERK1 ⁄ 2phosphorylation (Fig. 3D).Phosphorylation of Ser916 is neither...
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Tài liệu Báo cáo khoa học: Functional expression and mutational analysis of flavonol synthase from Citrus unshiu pptx
... flavonoid biosynthesis.We report the expression of highly active Citrus unshiuFLS in Escherichia coli and the purification of the labileenzyme by a modified protocol devised for the isolation of Petunia ... for FHT [9], and the proteinconcentration was adjusted to 0.371 mgÆmL)1in the sample. The documented spectra show the accumulation of 10 scans with 50 nmÆmin)1. The CD spectra of the FLS sample ... activityreached 0.5 mkatÆkg)1on average with either substrate, and the level of expression was equivalent for the wild-type and mutant FLSs except for the Gly68fiPro and Gly261fiPro mutants as determined...
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