Báo cáo khoa học: Thioredoxin Ch1 of Chlamydomonas reinhardtii displays an unusual resistance toward one-electron oxidation potx

... Thioredoxin Ch1 of Chlamydomonas reinhardtii displays an unusual resistance toward one-electron oxidation Ce´cile Sicard-Roselli1, Ste´phane Lemaire2, Jean-Pierre Jacquot3, ... Orsay, FranceTo test thioredoxin resistance to oxidizing free radicals, wehave studied the one-electron oxidation of wild-type thio-redoxin and of two forms with the point mutations D30Aand W35A, ... ateconstants of charge transfer d iffered b y a factor of t wobetween WT Trx and the D30A mutant (Table 1).The Trp•and TyrO•yields were estimated from themagnitudes of the absorbance changes...

Tài liệu Báo cáo khoa học: Membrane orientation of laminin binding protein An extracellular matrix bridging molecule of Leishmania donovani pdf

... mem-brane and subjected to Western blot analysis using anti-LBPIg, a single band at 67 kDa region was obtained (Fig. 2,lane 2), suggesting LBP to be one of the membrane proteins of Leishmania ... matrix(ECM) and basement membrane (BM). In the case of leishmaniasis, the causative parasite, Leishmania donovani,invades mammalian cells, primarily the resident macro-phages of liver and spleen, ... addressÕ [4–6]. Understanding of themechanisms mediating the adherence of L. donovani tothe ECM or host cells could lead to the development of antiparasitic agents whose mechanism of action wouldinvolve...

Báo cáo khoa học: Modulatory effects of plant phenols on human multidrug-resistance proteins 1, 4 and 5 (ABCC1, 4 and 5) potx

... P-glycoprotein and Mrp1 to basaldrug resistance. Cancer Res 60, 5761–5766.2 Gottesman MM, Fojo T & Bates SE (2002) Multidrug resistance in cancer: role of ATP-dependent transpor-ters. Nat Rev Cancer ... cassette transporters that are linked with anticancer and antiviral drug resistance and, as such, may be beneﬁcial in modulating drug resistance. This study investigates the interactions of six common ... such as quercetin and silymarin can modulate trans-port activities of MRP1, -4 and -5. Such interactions could inﬂuence bio-availability of anticancer and antiviral drugs in vivo and thus, should...

Báo cáo khoa học: "Serial Combination of Rules and Statistics: A Case Study in Czech Tagging" potx

... size of the set of possible and plausible tags can reachseveral thousands.Apart from agglutinative languages suchas Turkish, Finnish and Hungarian (see e.g.(Hakkani-Tur et al., 2000)), and ... standard evalua-tion techniques (and not even the same data). Butthe substantial disadvantage is that the develop-ment of manual rule-based systems is demandingand requires a good deal of ... following property: aword form wf1 can be part -of- speech determinedby means of another word form wf2 whose word-order distance cannot be determined by a ﬁxednumber of positions between the two word...

Báo cáo khoa học: "Generalized Encoding of Description Spaces and its Application to Typed Feature Structures" potx

... unifying the representatives of and , one ( ) for unifying the representatives of and , and one ( ) for unifying the representatives of and . Notice that the set of representatives formust be ... languages typically need to bindvariables to various substructures of a TFS, , andthen pass those variables outside the substructures of where they can be used to instantiate the value of another ... constraints anyway for encoding principles of grammar, so it is easy and computationally inexpen-sive to conduct this transformation.4 Static EncodabilityAs mentioned in Section 1, what we want is an...

Báo cáo khoa học: "Trainable Generation of Big-Five Personality Styles through Data-driven Parameter Estimation" potx

... naturalness of the outputs of our trained models. Table 9 showsthat the average naturalness is 3.98 out of 7, which issigniﬁcantly lower (p < .05) than the naturalness of handcrafted and randomly ... (4 out of 7). The goal is for eachutterance to project multiple traits on a continuousscale. To generate a range of alternatives, a Gaus-sian noise with a standard deviation of 10% of thefull ... for any arbitrary value on the varia-tion dimension scales. Additionally, our data-drivenapproach can be applied to any dimension that ismeaningful to human judges, and it provides an ele-gant...

Báo cáo khoa học: Biochemical characterization of a U6 small nuclear RNA-speciﬁc terminal uridylyltransferase potx

... primary transcripts. This two-step trans-esterification mechanism is performed by the spliceosome, an RNP complex of five small nuclear RNA molecules(snRNA U1, U2, U4, U5 and U6), and more than 60proteins ... significantly byhigher salt concentrations (150 mMKCl; lanes 3 and 8) andshowed no detectable activity at and above 200 mMKCl(lanes 4 and 5 and 9 and 10). Therefore, although slightdifferences between ... fractions of thetwo activities were tested in the presence of various concentrations of MgCl2(Mg++): 10 mM(lanes 1,5), 5 mM(lanes 2,6), 2.5 mM(lanes3,7) and 0.0 mMMgCl2(lanes 4,8)....

Báo cáo khoa học: Identiﬁcation of sodium salicylate as an hsp inducer using a simple screening system for stress response modulators in mammalian cells pptx

... induced the activation of HSF, the transcription of hsp genes and the accumu-lation of Hsps in various mammalian cells and a concomi-tant increase of thermoresistance of cells. Thus, SA maybe ... as Hsp105a and Hsp70 in various mammaliancells.Enhancement of thermoresistance of cells by SAUpon exposure to a sublethal heat treatment, mammaliancells acquire transient resistance to a subsequent ... theincrease of Luc activity by SA reﬂect the levels of transcription and translation of Hsp105, not due to an indirect effect of SA on the basal activity of Luc. Enhance-ment of Luc activity...

Báo cáo khoa học: Identiﬁcation of malic and soluble oxaloacetate decarboxylase enzymes in Enterococcus faecalis potx

... Tecnolo´gica (AN- PCyT, contract 15-38025, Argentina) and a EuropeanUnion grant (BIAMFood, contract KBBE- 211441).G. R. and P. M. are fellows of CONICET (Argentina),and M. E., V. B., S. A. and C. ... details). ME, MLEand OAD from LAB are highlighted withdashed circles and main branches A and Bare depicted as dashed rectangles. Boot-strap support values of main and minorbranches are indicated. ... variety of transporters and enzymes involved in themetabolism of organic compounds, such as malate andcitrate, encoded within its genome [13].In this work we identiﬁed two putative members of the...

Báo cáo khoa học: Hemagglutinin-33 of type A botulinum neurotoxin complex binds with synaptotagmin II potx

... structure and mode of action of botulinum neurotoxin on human health isserious and manifold. One of the most intriguing fea-tures of BoNTs is their existence as complexes with aset of NAPs [19,20].The ... membrane protein.FEBS Journal 272 (2005) 2717–2726 ª 2005 FEBS 2717light chains of BoNT ⁄ B, D, F and G cleave thevesicle-associated membrane protein (VAMP) [1]. Thecleavage of any one of the ... 66,50, 45 and 31 kDa under reducing conditions. Similaranalysis of the 0.5 m NaCl eluate revealed four proteinbands with molecular masses of approximately 90, 55,50 and 45 kDa. Western blot analysis...

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