Báo cáo khoa học: Neuroserpin Portland (Ser52Arg) is trapped as an inactive intermediate that rapidly forms polymers Implications for the epilepsy seen in the dementia FENIB ppt

... Neuroserpin Portland (Ser52Arg) is trapped as an inactive intermediate that rapidly forms polymers Implications for the epilepsy seen in the dementia FENIB Didier Belorgey1, ... neuroserpin was comparable to that obtained for other serpins, including a1-antitrypsin and a1-antichymo-trypsin [19,20]. In comparison, the spectrum of Ser52Arg neuroserpin shows an increase in ... eurose rpin, which is unusual for a serpin that s pontaneously forms polymers in vitro and in vivo [19]. The polymers of m utant n euroserpin that form in FENIB are analogo us to polymers that f...

Báo cáo khoa học: Discovery and characterization of a Coenzyme A disulﬁde reductase from Pyrococcus horikoshii Implications for the disulﬁde metabolism of anaerobic hyperthermophiles doc

... there is a blue shift in the 380 nmpeak and subsequent small increase in absorbance at340 nm, although the increase is much less than that expected for the amount of NADPH added (the increase ... nmand 400 and 500 nm. There is also an increase andblue shift in the peak which corresponds to the 380and 360 nm peaks of the E and EH2 forms, respect-ively. This result is inconsistent with ... however, that lit-tle if any of the enzyme is stabilized in the FADH2form. It was this ﬁnding that led us to originally con-clude that this enzyme was not likely to act as an NAD(P)H oxidase in...

Tài liệu Báo cáo khoa học: Metabolic control of mitochondrial properties by adenine nucleotide translocator determines palmitoyl-CoA effects Implications for a mechanism linking obesity and type 2 diabetes pdf

... 68% of the decrease in Jo, 68% of the decrease in Jp, 56% of the increase in the QH2⁄ Qratio, 70% of the increase in Dw, 72% of the increase in the ATP in ⁄ ADP in ratio, and 59% of the decrease ... were determined from yeast hexo-kinase kinetics as described [16]. As hexokinase kineticswere determined in medium containing creatine and creat-ine phosphate, this medium was used in all experimentswith ... of the reduction in Jo, 76% of the reduction in Jp, 68% of the increase in Dw, 88% of the increase in the ATP in ⁄ ADP in ratio, and 69% of the reduction in the ATPout⁄ ADPoutratio. The...

Báo cáo khoa học: Puriﬁcation, microsequencing and cloning of spinach ATP-dependent phosphofructokinase link sequence and function for the plant enzyme pdf

... microsequencing and cloning of spinachATP-dependent phosphofructokinase link sequence andfunction for the plant enzymeChristian Winkler, Britta Delvos, William Martin and Katrin HenzeInstitute ... reaction was initi-ated by addition of 0.4 mmd-fructose 6-phosphate, andactivity was determined by decrease in absorbance at340 nm. For discrimination between the chloroplast and cy-tosolic isoforms, ... reported the existence of chloroplast- andcytosol-speciﬁc isoenzymes in spinach. Since then, the isoforms of ATP-PFK from various plant sources havebeen studied [23–34]. Spinach cytosolic ATP-PFK is activated...

Tài liệu Báo cáo khoa học: Olfactory receptor signaling is regulated by the post-synaptic density 95, Drosophila discs large, zona-occludens 1 (PDZ) scaffold multi-PDZ domain protein 1 pptx

... domain binding motif in their C-termini.PDZ domains have been established as sites for protein–protein inter-action and play a central role in organizing diverse cell signaling assem-blies. In ... grouped into threemain classes of PDZ binding motifs, occurring at the C-terminus of the interacting proteins [16]. We scanned the human olfactory receptor repertoire for putativebinding motifs and ... (PDZ) domains, has the ability to bindto various molecules in the signal transduction cas-cade, thereby bringing them into close proximity andensuring a rapid and speciﬁc signal transduction[9,12].PDZ...

Tài liệu Báo cáo khoa học: Cytochrome P450 Cyp4x1 is a major P450 protein in mouse brain doc

... specific binding, the antisera were preincubatedwith purified recombinant Cyp4x1 protein, resulting in the ablation of detection of the 55-kDa protein in brain(Fig. 5C). This shows that the antisera ... the corresponding protein is translated in vivo. An antibodyspecific for the mouse Cyp4x1 protein was developed, as an antibody raised against human CYP4x1 showedpoor cross-reactivity against recombinant ... but that the oxidation of these is not directed, as is usually the case in the CYP4s, to the terminus of their carbon chain, but rather to someposition mid-way along the chain.Northern blot analysis...

Tài liệu Báo cáo khoa học: Rotary F1-ATPase Is the C-terminus of subunit c ﬁxed or mobile? docx

... thusexcluding any interchange of subunits. Nevertheless, it wasapparent that the ﬂuorescence intensity of the ac bands in all reoxidized samples was rather weak, a lthough the SDSband was very intense. ... cA285C wasgenerated by standard PCR with pKH7 as template DNAand using KpnIandSacI for transferring the PCR productinto pKH7 (resulting in plasmid pMM9). The mutationaP280C was generated using ... In a second mutant,containing just the point mutation within a, all subunits werelabelled with a ﬂuorescent d ye. Following disassembly andreassembly of the combined preparations and cystinereduction,...

Báo cáo khoa học: Arabidopsis thaliana CYP77A4 is the ﬁrst cytochrome P450 able to catalyze the epoxidation of free fatty acids in plants potx

... involved in the synthesis offatty acids in plastids, including the stearoyl acyl carrierprotein desaturase SSI2, and the plastidic long-chainacyl-CoA synthetase LACS9 (for a complete list ofco-expressed ... forma-tion by introducing the oxygen between carbon 9 and 10of oleic acid before incorporation of the monomer into the cutin. In this context, it is interesting to note that inhibition studies ... University of California, San Francisco, CA, USAFatty acid-oxidizing enzymes have been the subject of an increasing number of studies in all organisms, as the products of their reactions exhibit...

Báo cáo khoa học: VEGF gene expression is regulated post-transcriptionally in macrophages pdf

... coordinately regulated.Experimental proceduresCell culture, transfection and luciferase assay The RAW-264.7 cell line was obtained from ATCC(Manassas, VA) and maintained as described. Media wasDMEM ... underlined) in mouse is nonhomologous to human(human U-358, GenBank #AF024710). Following muta-genesis, the sequence of the VEGF-dL-luc was veriﬁed bysequencing on both strands and then cloned into ... arthritis and plaque formation in atherosclerosis. Vascular endothelial growth factor (VEGF) is an angio-genic cytokine that is produced by macrophages. To study the regulationof VEGF production in...

Báo cáo khóa học: ICAM-1 expression is highly NF-jB-dependent in A549 cells No role for ERK and p38 MAPK docx

... molecules as well as other enzymes, such as inducible nitric oxide synthase and cyclooxengenase-2,NF-jB may provide an attractive target for therapeuticintervention in inﬂammatory diseases [2]. ... agents that trigger exacerbation in airwaydiseases [15]. This epithelium is biosynthetically active andacts as a source of multiple in ammatory cytokines,chemokines, prostanoids and other mediators ... implicates the a andb isofroms of p38, as SB203580 has little or no effect onp38 c and d and it is possible that this could explaindiscrepancies between over-expression and inhibitor basedstudies...

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