Báo cáo khoa học: Akt-dependent phosphorylation negatively regulates the transcriptional activity of dHAND by inhibiting the DNA binding activity pdf

... speculatedthat phosphorylation of these sites might affect the DNA binding and/or dimerization activities of dHAND. We ﬁrstanalyzed the effect of phosphorylation on the transcriptional activity of dHAND ... the DNA binding activity of dHAND could beaffected by Akt phosphorylation. The inﬂuence of phos-phorylation by Akt on the DNA binding activity wasexamined by gel shift analysis. We included the ... within the bHLH domain. Phosphorylation by Akt inhibited the transcriptional activity of dHAND. We analyzed the mechanisms of this inhibitory effect of Akt-dependent phosphorylation on the transcriptional...

Tài liệu Báo cáo khoa học: Peptides corresponding to helices 5 and 6 of Bax can independently form large lipid pores pdf

... conditions [22], butwith the wall of the pore made of both lipid and pro-tein molecules. At the rim of the membrane hole, the two leaﬂets of the bilayer can make contact with eachother, forming a membrane ... permeabilizing activity of a pep-tide derived from the second helix of the putativepore-forming domain of Bax. We show that peptideswith the sequence of the ﬁrst (a5) and second (a6) heli-ces of the ... in the core of water-soluble folds of these proteins has been reported to be the pore-forming domain. Here we show that peptides including any of the twoa-helix fragments of the hairpin of...

Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt

... blots of lysates of Y394F tau(Fig. 2A). The absence of this larger species could indi-cate that phosphorylation of tau by Fyn is reducedand therefore, in addition to phosphorylation by c-Abl[25], ... interaction is regulated by serine ⁄ threonine phosphorylation of tau [20,21]. To determine whether the tyrosine phosphorylation status of tau also affects the binding of tau to Fyn-SH3, CHO cells ... predominantly by c-Abl. However,impaired phosphorylation of Tyr394 did not appearto inﬂuence the binding of tau to Fyn-SH2, as therewas no signiﬁcant difference in the amount of Y394Fthat...

Tài liệu Báo cáo khoa học: Disulﬁde bridge regulates ligand-binding site selectivity in liver bile acid-binding proteins ppt

... within the b-barrel, induced by the disulfide bridge, have beenmapped onto the BABP apo structure, and the effects of the binding of the two most abundant glycoconju-gated bile salts on the backbone ... GCDA. The comparison of 1H-spectra of the two protein samples at a pro-tein ⁄ GCDA ⁄ GCA ratio of 1 : 2 : 2 indicated that the final holo state is independent of the order of addition of the bile ... induced by disulfidebridge in the apo and holo forms of T91C L-BABP The effect of the disulfide bond introduction on the structure of the apo protein was investigated by moni-toring the 1H ⁄15N...

Tài liệu Báo cáo khoa học: Major phosphorylation of SF1 on adjacent Ser-Pro motifs enhances interaction with U2AF65 doc

... suggesting that phosphorylation of these sites might regulate the formation of the SF1-U2AF-RNA complex. We first tested the effect of phosphorylation on the interaction of SF1 withU2AF65 by GST pull-down ... in the presence of 1.2 lM U2AF65. The different bandswere quantified by phosphorimaging and the formation of the tern-ary complex was plotted as a fraction of total RNA. The decrease of the ... eachside of the proline-rich region containing the SPSPmotif [16]. Thus, local structural modifications of the SPSP region by phosphorylation could be amplified by these helical structures, thereby...

Tài liệu Báo cáo khoa học: Single phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2 confers high-afﬁnity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ-RGS3 protein ppt

... if the binding of one affects the binding of the other. We report here that phosphorylation of Tyr304 in the functional C-terminal region (residues 301–333) of ephrin B2confers high-affinity binding ... affinity of the binding interactions was evaluated by measuring the changes of fluorescence polarization of the peptides ateach step of titration with the Grb4 SH2 domain, i.e. the binding isotherms ... determine the binding specificity of the Grb4 SH2 in relation to the closest consensus binding sequence of the Src SH2 domain (i.e. pYEEI).Table 1. The effect of tyrosine phosphorylations on the binding...

Báo cáo khoa học: Amino acid limitation regulates the expression of genes involved in several speciﬁc biological processes through GCN2-dependent and GCN2-independent pathways ppt

... from the intestinal digestion of proteins [4,5].Another example of the detection of the lack of anamino acid is metabolic adaptation to cope with epi-sodes of protein malnutrition. In these ... in bold). The enrichment for a given GO category was computed as the ratio of the distribution of the amino acid-regulated genes* versus the distribution of the genes spotted onto the microarray**. ... suggesting that the reg-ulation occurs mainly at the transcriptional level. By contrast, the amount of pre-mRNA of Dusp16 is notaffected by amino acid starvation, but the amount of mature transcript...

Báo cáo khoa học: Insulin resistance in human adipocytes occurs downstream of IRS1 after surgical cell isolation but at the 1 level of phosphorylation of IRS1 in type 2 diabetes pot

... been the subject of intensive research for many years. Yet, wedon’t know the details of the molecular dysregulationin the target cells of the hormone. Studies of cells frompatients with the ... tyrosine- phosphorylation of the insulin receptor or of IRS1, or of phosphorylation of ERK1 ⁄ 2, was not signiﬁcantlydifferent in either group of diabetics compared with the nondiabetic controls (Table 2). The dose–response ... exhibitedlowered levels of phosphorylation (Fig. 1A,B). Insulintreatment now caused a signiﬁcant increase in the phosphorylation of ERK 1 and 2 (Fig. 1A), but hadno effect on the phosphorylation of p38 MAP-kinase(Fig....

Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

... counter. Values are the mean of six replicates. The speciﬁc uptake was determined by subtracting the uptake counts in the absence of inhibitorfrom the uptake counts in the presence of cocaine or S-cita-lopram, ... with the direct binding of p25a to vesicles suggest the pres-ence of vesicular p25a receptors, and this is now underinvestigation. The functional role of the vesicle interaction wasconﬁrmed by ... modulates the subcellular localization of the dopaminetransporter via effects on sorting vesicles, and thereby indirectly affects itscellular activity. Here we show that co-expression of the dopamine...

Tài liệu Báo cáo khoa học: Angiopoietin-like proteins: emerging targets for treatment of obesity and related metabolic diseases pptx

... stress) of adipose tissue. These ﬁndings support the possibilitythat the alteration of circulating ANGPTL2 levelscould serve as a marker of obesity-induced metabolicabnormalities. Furthermore, ... adiposetissue of ANGPTL2 null mice compared with that of wild-type mice [15]. These results raise the possibilitythat blocking ANGPTL2 signaling simultaneously sup-presses the expression of other inﬂammatory ... it is predicted that the suppression of ANGPTL2 signaling has few sideeffects. Therefore, for these reasons, we consider that the suppression of ANGPTL2 signaling as a therapeu-tic strategy...

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