Báo cáo khoa học: Two functionally redundant isoforms of Drosophila melanogaster eukaryotic initiation factor 4B are involved in cap-dependent translation, cell survival, and proliferation doc

... cultures and in developing tissues providedinteresting insights into the function of eIF4B. In mamma-lian cell c ultures, a 50-fold overexpression of eIF4B resulted in the inhibition of the protein ... of Drosophila melanogaster eukaryotic initiation factor 4B are involved in cap-dependent translation, cell survival, and proliferation Greco Herna´ndez1, Paula Va´zquez-Pianzola1, Andreas Zurbriggen2, ... results). Redundant function of Dm-eIF4B-L and Dm-eIF4B-S in translationTo study the effect of Dm-eIF4B isoforms in cap-dependent and IRES-dependent translation, we analyzed the effect of both proteins on the...

Báo cáo khoa học: Light-induced gene expression of fructose 1,6-bisphosphate aldolase during heterotrophic growth in a cyanobacterium, Synechocystis sp. PCC 6803 ppt

... Proteomeanalysis of light-induced proteins in Synechocystis sp.PCC 6803: identiﬁcation of proteins separated by2D-PAGE using N-terminal sequencing and MALDI-TOF MS. Mol Cells 10, 705–711.33 ... organism. For example, new insightsinto the role of thioredoxin in the regulation of cellularprocesses and the function of the cell membrane in cyanobacteria have been obtained through proteomicanalysis ... sTransport and binding proteins slr0559 Periplasmic binding protein of ABCtransporter for natural amino acids (NatB)10 ssll1447 Periplasmic protein, ABC-type ureatransporter system substratebinding...

Báo cáo khoa học: An alcohol acyl transferase from apple (cv. Royal Gala), MpAAT1, produces esters involved in apple fruit ﬂavor ppt

... transformed into C43 (DE3) cells [31].Expression of MpAAT1 recombinant protein in E. coliFor recombinant expression of protein, E. coli was grown in 500 mL 2YT broth in 3 L flasks inoculated ... 2-methylbutanol and butanol. Such kinetic characteristics of AATs may therefore be anotherimportant factor in understanding how the distinct flavor profiles of differ-ent fruit are produced during ripening.AbbreviationsAAT, ... acid, and dithiothreitol. These inhibitor results may reflect theproximity of cysteine residues in the substrate bindingpockets and ⁄ or catalytic region since zinc ions oftenuse cysteine residues...

Báo cáo khoa học: Aegyptin displays high-afﬁnity for the von Willebrand factor binding site (RGQOGVMGF) in collagen and inhibits carotid thrombus formation in vivo ppt

... panel).Aegyptin binds with high affinity to the vWFbinding site in collagen, independently of hydroxyproline In an attempt to identify the binding sites involved in collagen interaction with aegyptin, ... sufficient for the interac-tion.Aegyptin binds with low affinity to GPVI and integrin a2b1 binding sites in collagenSequences involved in collagen interaction with GPVI and integrin a2b1 were tested ... thrombusformation in the presence of Rose Bengal in vivo, without significant bleeding in rats. In conclusion, aegyptin interacts with distinct binding sites in colla-gen, and is useful tool to inhibit...

Báo cáo khoa học: The calcium-binding domain of the stress protein SEP53 is required for survival in response to deoxycholic acid-mediated injury pdf

... transfection, cells were split and plated in media containing Geneticin to select for cell containing vector DNA. After three weeks, the number of cells was determined byfixing cells and staining with ... in media containing Geneticin to select for cell containing vector DNA. After three weeks, the number of cells were determined by fixing cells and staining with dye and quantified in (H). The lower ... and developed cell lines to determine whe-ther the gene product had protective effects from DCAdamage in vitro.Cellular stress protein responses play a key role in minimizing cell injury and...

Báo cáo Y học: Molecular and biochemical characteristics of a gene encoding an alcohol acyl-transferase involved in the generation of aroma volatile esters during melon ripening pptx

... of thesame family.RESULTS AND DISCUSSIONSequence analysisBoth CM-AAT1 and CM-AAT2 encode proteins of 461amino acids with a theoretical molecular mass of 51.5 kDa and 51.8 and a pI of 8 and ... regulation of CM-AAT2, while other developmentalfactors are involved in addition to ethylene in the regulation of CM-AAT1. Application of the 1-MCP to WT fruitsstrongly inhibited the expression of ... X-rayﬁlms and developed a few hours later.Expression of CM-AAT1 and CM-AAT2 in yeastBoth CM-AAT1 and CM-AAT2 cDNAs were cloned in thepYES1.2 TOPO-TA cloning vector and yeasts (strainINVSc1)...

Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx

... kcat and the ETF interaction are relatively unaffected. This mutation seems to affectmainly the initial folding and stability of the tetramer, and similarly lowered levels of tetramer in human cellswould ... theefficiency of the chaperonin-aided folding, the effi-ciency of the detoxification of accumulated intermedi-ates, and avoidance of exposure to the environmentaltriggers. Certainly in the case of MCAD ... Ireland BV (Cork,Ireland).Mutagenesis and subcloning of R256T and K364RThe genes for the mutant MCAD proteins were overex-pressed in E. coli JM109 cells by using the pWT vector(encoding...

Tài liệu Báo cáo khoa học: "TWO KINDS OF METONYMY" pdf

... flight -of, (wh airlines), airline -of pp: orig -of, Boston, identity pp: dest -of, Denver, identity This representation has three bindings: a SUBJECT and two PP-complements. In the two PP bindings, ... modifier bindings of the NP itself as its "intemal" bindings. In this particular example, there are no internal modifiers and thus no internal bindings. When an NP is a constituent of ... flight -of x meal -of dinner))) where coercion relation is AIRCRAFT -OF, mapping between flights and the aircraft they are on. Compare this with the reading for (6), in which airlines and not...

Tài liệu Báo cáo khoa học: "Two-Level, Many-Paths Generation" docx

... currency in the domain, even if they are rough paraphrases. In short, knowledge-based generators are faced with multiple, complex, and interacting lexical constraints, 3 and the integration of ... Each island cor- responds to an independent component of the final sentence. Each individual word in an island specifies a choice point in the search and causes the creation of a state in the ... analyzer, and (2) knowledge gaps in the gen- erator itself. These two categories of gaps include: • Interlingual analysis often does not include ac- curate representations of number, definiteness,...

Tài liệu Báo cáo khoa học: "Two Constraints on Speech Act Ambiguity" potx

... come to integrate the two into a complete system. 4.1. Interaction of the Constraints The plan reasoning phase constrains the results of the linguistic computation by eliminating interpretations, ... will look at rules using all of these kinds of information, introducing the notation for rules as we go. Rules consist of a set of features on the left-hand side, and a set of partial speech ... interpretations, and reinterpreting others. The linguistic computation constrains plan reasoning by providing the input; the final interpretation must be in the range specified, and only if there...

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