Báo cáo khoa học: Plant DNA polymerase k, a DNA repair enzyme that functions in plant meristematic and meiotic tissues docx

... Plant DNA polymerase k, a DNA repair enzyme that functions in plant meristematic and meiotic tissues Yukinobu Uchiyama, Seisuke Kimura, Taichi Yamamoto, Toyotaka Ishibashi and Kengo SakaguchiDepartment ... from a higher plant, rice, using cDNA and recombinant proteins.The results suggest that plant DNA Pol k is a DNA repair enzyme which functions in plant meristematic and meiotic tissues. Materials ... 5¢-AGCTACCATGCCTGCACGAAGAGTGCGTATTATGCCTACACTGGAGTACCGGAGCATCGTCGTGACTGGGAAAAC-3¢.[3H]dTTP (10 lM;10CiÆmmol)1) and enzymes were addedas indicated in the ﬁgure legends, and incubated...

Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: a capacitative (AC) electrical coupling model in neuroepithelium pptx

... withF-actin via b-catenin and a- catenin; b-catenin bindsto cadherin and a- catenin, which in turn interacts withF-actin (Fig. 3A) [24]. Thus b-catenin plays a pivotalrole in the regulation of ... in intracellular [Ca2+], as described below.Synchronous increases in intracellular [Ca2+] and disassembly of cadherin–catenin complexesThe cytoplasmic domain of cadherin interacts withF-actin ... space,because ICcan pass the plasma membrane capacita-tively. Thus, the current loop can be closed via theextracellular space and the plasma membrane. Thismay allow capacitative electrical...

Báo cáo khoa học: Fowlicidin-3 is an a-helical cationic host defense peptide with potent antibacterial and lipopolysaccharideneutralizing activities ppt

... highlypotent against a broad range of Gram-negative and Gram-positive bacteria in vitro, including antibiotic-resistant strains, with minimum inhibitory con-centrations in the range 1–2 lm. It kills bacteria ... evaluate theantibacterial spectrum and efﬁcacy of fowlicidin-3, weperformed standard broth microdilution assays in 100% Muller-Hinton broth as recommended by theClinical and Laboratory Standards ... Standards Institute [15] usingfowlicidin-1 as a reference peptide. As shown in Table 2, fowlicidin-3 was active against a wide rangeof Gram-negative and Gram-positive bacteria withminimum inhibitory...

Báo cáo khoa học: The propeptide of cruzipain ) a potent selective inhibitor of the trypanosomal enzymes cruzipain and brucipain, and of the human enzyme cathepsin F ppt

... peptide, a prodomain, and a maturedomain that retains catalytic activity. In trypanosomalCPs, the mature enzyme is composed of a centraldomain that corresponds to the mature domain ofmammalian ... described as highly select-ive for the parasite’s enzymes, being practically inca-pable of inactivating mammalian cathepsins L, K and B and papain [27]. In another study, the inhibitorypotential ... in our assays.Cathepsin F is a key enzyme responsible for the deg-radation of the invariant chain (Ii) in macrophages,playing a fundamental role in antigen presentation[34]. Cathepsin F also...

Báo cáo khoa học: "LEARNING TRANSLATION SKILLS WITH A KNOWLEDGE-BASED FRENCH-ITALIAN CONJUNCTIONS IN CONTEXT" pdf

... education- al settings and in projects of natural language translation. Practically, our program is one of the few Intelligent Systems available in the field of Fo- reign Language Teaching and ... structures. The main achievement of DART was the integration of tradi- tional Computer Assisted Learning (CAL~ facilities - such as the ones available in the PLATO system - in an Artificial Intelligence ... concepts and skills based upon state-of-the-art research in Intelligent reaching Systems and Computational Linguistics. The tutor is part of a large R&D project in ITS which resulted in a system...

Báo cáo khoa học: Planarian peptidylglycine-hydroxylating monooxygenase, a neuropeptide processing enzyme, colocalizes with cytochrome b561 along the central nervous system pptx

... Dugesia japonica collected in the Irima River in Gifu, Japan and maintained as described (clonal strain: GI)[58].Cloning and sequencing of planarian PHM cDNA A cDNA library of the planarian made ... (5¢-AAAC TGCA GAGA AATT GGTAAG AAATC TCC- 3¢) and spPAM-S3 (5¢-TCAGGATCCAGATTCTTTAGCTGCCAGATG-3¢) –were synthesized. The former primerhad a PstI site as an adaptor. The 3¢ end of the cDNA wasampliﬁed ... evolutionary origin of a brain: planarian brain characterized by microarray.Mol Biol Evol 20, 784–791.44 Mineta K, Nakazawa M, Cebria´F, Ikeo K, Agata K &Gojobori T (2003) Origin and evolutionary...

Báo cáo khoa học: "Four Techniques for Online Handling of Out-of-Vocabulary Words in Arabic-English Statistical Machine Translation" docx

... (Habash and Rambow, 2005), and TOKAN, a general Arabic tokenizer (Sadat and Habash, 2006). English preprocessing simply in- cluded down-casing, separating punctuation fromwords and splitting ... preprocessing of the training and test data reduces data sparsity and OOV rates. Thisis especially true for languages with rich morphol-ogy such as Spanish, Catalan, and Serbian (Popovi´c and Ney, ... 2004) and Arabic (Sadat and Habash,2006). We are interested in the speciﬁc task ofonline OOV handling. We will not consider solu-tions that game precision-based evaluation metricsby deleting...

Tài liệu Báo cáo khoa học: C-Terminal extension of a plant cysteine protease modulates proteolytic activity through a partial inhibitory mechanism doc

... of papain and actinidin, and on aminoacid sequence information for cathepsins B and H, and stem bromelain. J Mol Biol 182, 317–329.6 Yamada K, Matsushima R, Nishimura M & Hara-Nishimura ... cysteine proteases [Erv -A, -B and –C (isolated from thelatex of Ervatamia coronaria in our laboratory) and papainfrom Carica papaya (Merck, Kenilworth, NJ, USA)], twoserine proteases [trypsin and ... activated matureprotein thus generated in each case shows a band atthe same position ( 27 kDa) like that in the autoacti-vated enzyme, as observed in SDS ⁄ PAGE analyses(Fig. S1), indicating that...

Tài liệu Báo cáo khoa học: The stereochemistry of benzo[a]pyrene-2¢-deoxyguanosine adducts affects DNA methylation by SssI and HhaI DNA methyltransferases pptx

... benzo [a] pyrene-2¢-deoxyguanosineadducts affects DNA methylation by SssI and HhaI DNA methyltransferasesOksana M. Subach1, Diana V. Maltseva1, Anant Shastry2, Alexander Kolbanovskiy2,Saulius Klimasˇauskas3, ... dissociation constant; M.SssI, SssI DNA methyltransferase;M.HhaI, HhaI DNA methyltransferase; MTase, DNA methyltransferase; V0, initial rate of methylation; Vmax, maximal rate of methylation.FEBS ... dG-(-)-anti-BPDE. Biochemistry 34, 13582–13593.41 Wyszynski MW, Gabbara S, Kubareva EA, RomanovaEA, Oretskaya TS, Gromova ES, Shabarova ZA &Bhagwat AS (1993) The cysteine conserved amongDNA...

Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

... of mtDNA. It wasproposed that in addition to a role in initiating a rolling-circle DNA replication, recombination could be instrumen-tal in resolving concatamers into linear monomers in theprocess ... Holliday junctions in mtDNA fromhuman heart muscle was recently demonstrated [38]suggesting that recombination intermediates and enzymes that resolve them could be involved in maintaining thestability ... and 2 while leaving the interacting a- helix 8 intact, may destabilize the proteins leading tospurious protein–protein or /and protein DNA interactions.The crystal structure of Ydc2 shows that...

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