Báo cáo khoa học: Copper-containing nitrite reductase fromPseudomonas chlororaphis DSM 50135 Evidence for modulation of the rate of intramolecular electron transfer through nitrite binding to the type 2 copper center pot
Báo cáo khoa học: Copper-containing nitrite reductase fromPseudomonas chlororaphis DSM 50135 Evidence for modulation of the rate of intramolecular electron transfer through nitrite binding to the type 2 copper center pot
... Copper- containing nitrite reductase fromPseudomonas chlororaphis DSM 50135 Evidence for modulation of the rate of intramolecular electron transfer through nitrite binding to the type 2 copper ... Biochem. 27 1) 23 67potential. However, addition of nitrite to the reducedform of the enzyme causes more complete reoxidation of the T1Cu than of the T2Cu center (cf. Fig. 4), indicatingthat the electron ... favoring the electron- transfer process. Analysis of the EPR spectrum of the turnover form of the enzyme also suggests that the electron- transfer process between T1Cu and T2Cu is the fastest of the...
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Tài liệu Báo cáo khoa học: Diol dehydratase-reactivating factor is a reactivase – evidence for multiple turnovers and subunit swapping with diol dehydratase pdf
... 100Ca 2+ 0. 52 ± 0.01 26 Cr 2+ a0.38 ± 0.01 19Mn 2+ 2. 23 ± 0.01 1 12 Co 2+ 2. 14 ± 0.04 107Ni 2+ 2. 42 ± 0.09 122 Cu 2+ 0.80 ± 0.05 40– 0.54 ± 0. 02 27aCr 2+ added might be oxidized to Cr3+by ... by the reactivase in the presence of ATP and Mg 2+ at a molar ratio of the reactivase to the enzyme of 0.1 is shown in Fig. 3. From the initial rate, the rate constant of the reactivase in the ... to the resulting mixture in a total volume of 6 lL, and the mixture was incubated at 20 °C for 20 min to form the ADP-bound form of the reactivase. The enzymeÆCN-Cbl complex was formed by the...
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Báo cáo khoa học: Interaction between catalytically inactive calpain and calpastatin Evidence for its occurrence in stimulated cells docx
... to occur at higher concentra-tions of the metal ion [45]. The addition of E64, a syn-thetic inhibitor of calpain, did not modify the intensity of the signal observed in the presence of Ca 2+ alone. The ... direct evidence of the nature of the ligand responsible for the observed conformationaltransition. Thus, to discriminate between the effect due to the rise in free Ca 2+ from that induced by the ... monitored by evaluating the intensity of the light signal generated by the binding of mAb56.3. To perform these investigations, the two preferentialligands of calpain, Ca 2+ ions and calpastatin,...
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Báo cáo khoa học: "GRAMMIATICAL AND UNGRAMMATICAL STRUCTURE SINUSER - ADVISER DIALOGUES1 EVIDENCE FOR SUFFICIENCY OF RESTRICTED LANGUAGE SINNATURAL LANGUAGE INTERFACES TO ADVISORY SYSTEMS." potx
... attach to NPs rather than VPs and moreover attach most often to the lowest, nearest NP is important for the semantic interpreta- tion of sentences because of the combinatorial explosion of possible ... affect the form of the dis- course. Examples of dimensions arc: interaction, the extent to which user and adviser can quickly interact, respond to each other; involvement, the extent to which the ... used to perform operations with the statistical package and the lower window was used to type utterances to the adviser. The participants were instructed to ask help in English from what they...
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Báo cáo khoa học: Saporin and ricin A chain follow different intracellular routes to enter the cytosol of intoxicated cells pptx
... Journal 27 2 (20 05) 4983–4995 ª 20 05 FEBS 4983sequence found at the C-terminus of PEA is essential for the cytotoxicity of the endocytosed toxin, allowingPEA to reach its site of action [2, 5]. ... Journal 27 2 (20 05) 4983–4995 ª 20 05 FEBS 4985 of intoxication. Neither the human leukemic cell lineHSB -2 nor the Burkitt lymphoma Ramo cells showedany potentiation of cytotoxicity by addition of the KDEL ... (Table 2) . Vero cells aregreatly sensitized to RTAKDEL [10– 12] and weretherefore used to compare the cytotoxic activities of the recombinant polypeptides (Table 2) . The KDELsequence increased the...
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Báo cáo khóa học: UDP-galactose 4-epimerase from Kluyveromyces fragilis Evidence for independent mutarotation site pdf
... FEBS 20 03protection of either of the activities was observed (Fig. 4C).SDS/PAGE of the digest demonstrated the disappearance of the parent molecule, with generation of the two fragments of 45 ... orremoval of the equatorial OH on C2 is known to abolish allsubstrate interactions. 2- Deoxy-D(+)-glucose and 2- deoxy-D(+)-galactose, which act as inhibitors for the kidneyenzyme, appeared to be ... UDP-galactose into UDP-glu-cose, is the first enzyme of the ÔLeloir pathwayÕ of galactosemetabolism [1 ,2] . Clinically, this is related to the diseaseÔgalactosemiaÕ [3]. It belongs to the rare...
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Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase–methionine synthase reductase complex docx
... electrostatic potentials of the surface of the CPR FMN domain and of a model of the FMN domain of human MSR.Fig. S3. Electrostatic potentials of the surface of the hMS AD.Table S1. Sequences of oligonucleotides ... (AqCbl) to form cob(II)alamin. Reduction of the Co centrepromotes the dissociation of the lower dimethylbenz-imidazole base of the cofactor. Consistent with this, the crystal structure of the cobalamin -binding ... couple (determined for MetH [20 ]), which equates to free energy changes of 36 and 26 kJÆmol)1, respectively [21 23 ], for electron transfer between the two cofactors.We examined whether reductive...
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Báo cáo khoa học: Aldo-keto reductase from Helicobacter pylori – role in adaptation to growth at acid pH doc
... beyond 10 h of growth, as compared to the wild -type. The growthrates of both the wild -type and the mutant were com-promised at pH 5.0 (not shown). The addition of urea (10 mm) to the medium ... growth for both the wild -type and the mutant (data not shown).There was also a marked increase in the pH of the medium after 48 h of bacterial growth for both the wild -type and isogenic mutant. The ... and ketones to the corre-sponding alcohol product, and thus serve to removepotentially cytotoxic and mutagenic aldehydes fromcells [14].In view of the potential aldehyde detoxifying role of AKR...
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Tài liệu Báo cáo khoa học: A DExD⁄ H box RNA helicase is important for K+ deprivation responses and tolerance in Arabidopsis thaliana docx
... of an Arabidopsis DExD ⁄ H box RNA helicase R R. Xu et al. 23 02 FEBS Journal 27 8 (20 11) 22 96 23 06 ª 20 11 The Authors Journal compilation ª 20 11 FEBSwe applied this technique to clarify genotype ... plates. Seeds were stratified at4 °C for 2 days, and then transferred to 22 °C for 2 weeks.Col-0 was used as the wild type, and was the genetic back-ground for transgenic plants. Helps (SALK_118579,At3g46960) ... orcawu@sdau.edu.cn(Received 26 January 20 11, revised 22 April 20 11, accepted 28 April 20 11)doi:10.1111/j.17 42- 4658 .20 11.08147.x The molecular mechanism for sensing and transducing the stress signals...
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Tài liệu Báo cáo khoa học: It is all about resolution Meeting report based upon presentations at the 10th International Global BioMillennium 2006 symposium on molecular cell biology (Tbilisi, Georgia) docx
... Department of BiologicalChemistry, The Hebrew University of Jerusalem, Jerusalem 91904, IsraelFax: +9 72 2 6 52 025 8Tel: +9 72 2 658 5109E-mail: soreq@cc.huji.ac.il(Received 23 August 20 06, revised 20 November ... resolutionFEBS Journal 27 4 (20 07) 924 – 927 ª 20 07 The Authors Journal compilation ª 20 07 FEBS 925 reported by Hansjo¨rg Hauser (Braunschweig, Ger-many). In the absence of interferon, STAT2 perma-nently ... between the cytoplasm and the nucleus. A region in the C-terminus of STAT2controls its specific export in the absence of interferon.STAT1 also shuttles in the absence of interferon, but the exchange...
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