Báo cáo khoa học: Vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide attenuate the cigarette smoke extract-induced apoptotic death of rat alveolar L2 cells ppt

... Biochem. 271) 1767 Vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide attenuate the cigarette smoke extract-induced apoptotic death of rat alveolar L2 cells Satomi ... study of rat lung alveolar L2 cells, cigarette smoke extract (CSE) induced apoptotic cell death. Expo-sure of L2 cells to CSE at a concentration of 0.25%resulted in a 50% increase of caspase-3 and ... PACAP27 on the CSE-induced cyto-toxicity in rat alveolar L2 cells. The L2 cells were ﬁrstisolated and cloned from adult rat alveolar epithelial cells using clonal selection techniques. L2 cells...

Báo cáo khoa học: Galanin-like peptide and the regulation of feeding behavior and energy metabolism pptx

... neurons project from the ARC to the PVN, and the other in which they project to the MPA, bed nucleus of the stria terminalis and lateralseptal nucleus.Central administration of GALP activates ... regions of the rat brain. Injection of GALPinto the third ventricle induces c-Fos expression, amarker of cell activation, in the horizontal limb of the diagonal band of Broca, POA, ARC and median ... consider the therapeutic value of the intrana-sal administration of GALP. In addition, this reviewwill provide an overview of a novel peptide, alarin,generated by alternative splicing of the GALP...

Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc

... presenting the pulse of Src activity in the plane of the inactive and active fractions, si and s1. If the duration of the stimulus exceeds the critical value, the trajectory in the (si, ... addition, the loss of a Z-shape by the QSS curve and, therefore, the lack of complex dynamicregimes can result from increases in (a) b ¼ K4=Stot; (b) the ratio n of quasi steady-state concentrations ... s2 and s1; and (c) the rate constants k3 and k7(see, Fig. S3C–F).This analysis of the parameter variation effects on the QSS curve is useful for experimental manipulations of the concentrations...

Tài liệu Báo cáo khóa học: Active-site residues and amino acid speciﬁcity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx

... the temperature from 30 to 42 °C hasFig. 5. Analysis of the enzymatic activity of His-CoaB R206Q and A276V. The synthesis of 4¢-phosphopantothenoyl-CMP (–cysteine, upper part of the figure) and ... catalyze the biosynthesis of polypeptide antibiotics [1]. Lipmann discovered and characterizedcoenzyme A [2] and Lynen elucidated that the thiolgroup of the cysteamine moiety of coenzyme A is the functional ... fromD-pantothenate. The Dfp flavoprotein catalyzes the synthe-sis of the coenzyme A precursor 4¢-phosphopantetheinefrom 4¢-phosphopantothenate and cysteine using the cofac-tors CTP and flavine...

Báo cáo khoa học: Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis A key factor participating in natural rubber biosynthesis pdf

... that of the control experiment withWBP alone. On the other hand, addition of WBP to the homogenate of BL21(DE3)/pETHRT2 resulted in aremarkable increase in the amount of IPP incorporationin the ... Overexpression of HRT1 and HRT2 in E. coli and puriﬁcation of HRT2. The pETHRT1 and pETHRT2 were constructed and introduced into E. coli BL21(DE3). The expression of these genes wereinduced by addition of ... coliBL21(DE3)/pETHRT1 with IPTG; S2, supernatant of the homogen-ate of E. coli BL21(DE3)/pETHRT2 with IPTG; PC, pellet of the homogenate of E. coli BL21(DE3)/pET32b without IPTG; P1, pellet of the...

Báo cáo khoa học: Super life – how and why ‘cell selection’ leads to the fastest-growing eukaryote doc

... (L·h–1)Dilution rate, D (L·h–1)Time, t (h)Time, t (h)Total amount of cells Number of cells (cells L–1) × 1011Number of cells (cells L–1) × 1011Fig. 4. Simulations of the dilution rate (bold ... of generations asdetermined by the number of bud scars. Our calcula-tion of s ⁄ v ratios on the basis of these data [87]revealed a relative decrease in the s ⁄ v ratio of 24%within the ﬁrst ... to6.3. The buffering capacity of the fresh medium in the reservoir was determined at laboratory temperature (20–25 °C). The pH of the reservoir medium was determined and the volume of 0.10...

Báo cáo khoa học: "Large-Scale Induction and Evaluation of Lexical Resources from the Penn-II Treebank" potx

... the left (the preﬁx) and the right (sufﬁx) context of the head for eachsyntactic category occurring as the mother node of such heads. To give a simple example, the rightmostNP to the left of ... name”) The value of the :cs feature is the constituent struc-ture of the subcategorisation frame, which lists the syntactic CF-PSG constituents in sequence. The value of the :gs feature is the ... fea-ture to the generated f-structures, the value of whichis the syntactic category of the pred at each level of embedding. Exploiting this information, the ex-tracted semantic form for the verb...

Báo cáo khoa học: Puriﬁcation, characterization and molecular cloning of tyrosinase from the cephalopod mollusk, Illex argentinus docx

... properties of the squid tyrosinaseEffects of pH and temperature on stability and acti-vity. As ST94 and ST94t showed the identical results onpH- and temperature-effects, we describe the data of ST94t ... taken from the literature [43]. The Km and Vmaxvalues for different substrates were obtained from the Hanes–Woolf equation.Extraction of RNA and first strand cDNA preparationAll DNA and RNA ... at the isosbestic point wavelength of each MBTH-adduct at 25 °C, and the steady-state rate of oxidation of substrate was determined using the molarextinction coefficient of MBTH-adduct at the...

Tài liệu Báo cáo khoa học: Tachykinin-related peptide precursors in two cockroach species Molecular cloning and peptide expression in brain neurons and intestine docx

... demonstrated the occurrence of the different TKRPs in single glomeruli of the tritocerebrum and in cells of the optical lobe.AbbreviationsESI-Q-TOF MS, electrospray ionization quadrupole time -of- ﬂight ... demonstrated the occur-rence of the different TKRPs in glomeruli of the trito-cerebrum and in cells of the optical lobe.ResultsCloning of cockroach preprotachykinin cDNAsUsing degenerated ... of copies of different TKRPs of all inverte-brates studied so far. Most of the 14–15 peptides pre-dicted on each of the precursors could be identiﬁed in the brain and intestine of the two cockroach...

Báo cáo khoa học: Characterization of structural and catalytic differences in rat intestinal alkaline phosphatase isozymes pdf

... the active site of the enzymes and studied the effect of various combinations of metals at the activesite. The models showed the possibility of a Zn triadat the metal-binding positions of the ... comparison of the residues serving as the ligand for the M3 metal ion is shown in Table 1. The residues of the direct ligands to the metal were the same as in hPLAP, but those of indirect ligands,which ... related to the coordi-nation of the metal at metal-binding site 3 and water molecules. These ﬁnd-ings suggest that the side-chain position of His153, and the alignment of Q317, might be the major...

Xem thêm