Báo cáo khóa học: SUT2 is a novel multicopy suppressor of low activity of the cAMP/protein kinase A pathway in yeast docx

Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf

... similar to that in Asp141 of Met8P. The idea of NirF being a dehydrogenase is appealingbecause of the presence of a putative nucleotide-bind-ing motif in the N-terminal of the protein sequenceand ... is the His residue that in NirS is the proximal axial ligand to the d1heme.Replacement of an equivalent His, His41, in NirF byAla abolished binding of the heme to the protein.Known distal ... seek accu-mulation of the substrate of NirF in a mutant thatlacks NirF; this too is not trivial as the DnirF straindoes not accumulate readily detectable amounts of anintermediate of d1synthesis.Experimental...

Tài liệu Báo cáo khoa học: Bacitracin is not a speciﬁc inhibitor of protein disulﬁde isomerase pptx

... able to catalyze the reduction of disulﬁde bonds. The effects of bacitracin on this activity were examined using the insulin precipitation assay. In the noncatalyzed assay, the disulﬁdes of insulin ... Escherichia coli DsbA and DsbC,as well as the isolated catalytic a domain of PDI. Both the PDI a domain and DsbA have a catalytic site, withan associated substrate-binding site, but lack an inde-pendent ... contamination of some commer-cially available bacitracin preparations [31].Here, we have tested the effect of bacitracin in a variety of in vitro assays for PDI activity. On the basis of the...

Báo cáo khoa học: Lpx1p is a peroxisomal lipase required for normal peroxisome morphology potx

... Woolford CA, Noble JA, Garman JD, Tam MF, InnisMA & Jones EW (1993) Phenotypic analysis of protein-ase A mutants. Implications for autoactivation and the maturation pathway of the vacuolar hydrolases ... chain fattyacids, in a- oxidation, bile acid and ether lipid synthe-sis, and in amino acid and purine metabolism [1].Peroxisomes are a source of reactive oxygen species,and are involved in the ... images of a deletion of Lpx1p(peroxisomes containing inclusions or invaginations) is symptomatic of a yeast peroxisomal mutant, and is reminiscent of the phenotypes found in human peroxi-somal...

Tài liệu Báo cáo khoa học: It is all about resolution Meeting report based upon presentations at the 10th International Global BioMillennium 2006 symposium on molecular cell biology (Tbilisi, Georgia) docx

... DAPs (death -associated proteins), that differ substantially in theirbiochemical properties and intracellular localization.DAP-kinases (DAPk), Ca2+⁄ calmodulin-regulatedand Ser ⁄Thr kinases, ... by AaronCiechanover (Haifa, Israel), who argued that the ubiquitin proteolytic system covers the pathway forelucidating the basic mechanisms that are pivotal fordrug targeting. Degradation of ... of cellular proteins playsmajor roles in a multitude of basic pathways duringcell life and death, in both health and disease. The ubiquitin–proteasome pathway involves conjugation of multiple...

Báo cáo khoa học: Visfatin is induced by peroxisome proliferator-activated receptor gamma in human macrophages pdf

... experiments, increasing amounts (5, 10, 50, 100 and200-fold excess) of unlabeled visfatin–PPREwt (5¢-CAATACAGGGCAAAGATCATGGAAG-3¢) or visfatin–PPRE-mut (5¢-CAATACAGGAAAAAGAAAATGGAAG-3¢) oli-gonucleotides ... increases the intracellular NAD+concentration in human macrophagesAs visfatin is known as a nicotinamide phosphoribosyltransferase [2], we investigated whether the induction of visfatin by PPARc affects ... process.As visfatin is the rate-limiting enzyme for the con-version of nicotinamide to NAD+ in mammals, the increased concentration of intracellular NAD+induced by PPARc agonists is probably the...

Báo cáo khoa học: YidC is required for the assembly of the MscL homopentameric pore potx

... acid disodium salt (AMS) werepurchased from Invitrogen (Carlsbad, CA, USA). Antise-rum against in uenza haemagglutinin (HA) was obtainedfrom Sigma (St Louis, MO). The other antisera used werefrom ... 5¢-GCGCGCGAATTCATGAGCATTATTAAAGAATTTCG-3¢ (forward)and 5¢-CGCGCGGGATCCTTAAGCATAATCAGGAACATCATAAGGATAACCACCAGGAGAGCGGTTATTCTGCTCTTTC-3¢ (reverse). The EcoRI ⁄ BamHI-digestedPCR fragment (MscL–HA) was cloned into pC4Met [31]. ... membranepreparations, indicating that the protein is fully inte-grated into the lipid bilayer (Fig. 4A, right panels).This corroborates our results from the SCAM assay,and again suggests that neither...

Báo cáo khoa học: Gene expression silencing with ‘speciﬁc’ small interfering RNA goes beyond speciﬁcity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx

... (forward, 5¢-GGCCCAGGTGACTCAGCTATT-3¢; reverse, 5¢-AGGGCATCCGAGAATTCCTT-3¢), LAMP2 (forward, 5¢-TCAGCATTGCAAATAACAATCTCA-3¢; reverse, 5¢-CAGTCTGCTCTTTGTTGCACATATAA-3¢), CTGF (forward, 5¢-CAAGCTGCCCGGGAAAT-3¢; ... samples. The presence of the internal standard probesat different locations of the array allowed quantiﬁcation of the local background and evaluation of the array homoge-neity, which is taken ... -CCCAGTGAGGATTGGATGAACTA-3¢), SPARC (forward, 5¢-GAGACCTGTGACCTGGACAATG-3¢; reverse, 5¢-GGAAGGAGTGGATTTAGATCACAAGA-3¢), TGFBR2 (forward, 5¢-TGGACCCTACTCTGTCTGTGGAT-3¢; reverse, 5¢-TTCTGGAGCCATGTATCTTGCA-3¢)...

Báo cáo khoa học: An acridone-producing novel multifunctional type III polyketide synthase from Huperzia serrata pot

... [20–23]. As in the case of S. baicalensis CHS, Hu. serrata PKS1 also accepted a variety of aromatic and aliphatic CoAs as startersubstrates, and produced aromatic tetraketides afterthree condensations ... 1079cDNA cloning The Hu. serrata plant used in this study was obtained fromL Hua (Zhejiang Academy of Medical Sciences, Hangzhou,China). Total RNA was extracted from young leaves of Hu. serrata and ... of the enzyme, and guides the course of the Claisen-type aromatic ring formationreaction of the enzyme-bound tetraketide intermediate. In particular, it is remarkable that Hu. serrata PKS1accepted...

Tài liệu Báo cáo khoa học: Hepatic stimulator substance mitigates hepatic cell injury through suppression of the mitochondrial permeability transition pdf

... characterized and the most prominent path-ways are the extrinsic and intrinsic pathways. In the intrinsic pathway (also known as the ‘mitochondrial pathway ), apoptosis results from an intracellular ... effect of HSS against CCCP-induced apoptosis, the activation of caspase-3 was examined using the enzymatic Caspase-Glo 3 ⁄ 7 assay. After CCCP treat-ment, caspase-3 activity increased markedly in ... from the inter-membrane space, triggering activation of caspases andapoptosis [41,44].Apoptosis is a genetically predetermined mechanismthat may be activated by several molecular pathways.The...

Tài liệu Báo cáo khoa học: Pronounced adipogenesis and increased insulin sensitivity caused by overproduction of prostaglandin D2 in vivo pptx

... 5¢-GGAGATCTCCAGTGATATCGACCA-3¢ and 5¢-ACGGCTTCTACGGATCGAAACT-3¢ for PPARc ,5¢-AAGACAGCTCCTCCTCGAAGGTT-3¢ and 5¢-TGACCAAATCCCCATTTACGC-3¢ for aP2,5¢-ATCCATGGATGGACGGTAACG-3¢ and 5¢-CTGGATCCCAATACTTCGACCA-3¢ ... University,Japan4 Laboratory of Biodefense and Regulation, Osaka University of Pharmaceutical Sciences, JapanIntroduction The amount of adipose tissue in the body is an impor-tant factor in the maintenance ... 5¢-GCGTCGGGTAGATCCAGTT-3¢ and 5¢-CTCAGTGGGGCTTAGCTCTG-3¢ for ACC, and 5¢-AACACCCCAGCCATGTACGTAG-3¢ and 5¢-TGTCAAAGAAAGGGTGTAAAACGC-3¢ for b-actin. Expression levels of the target genes were normalized to that of...

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