Báo cáo khoa học: Identiﬁcation and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

... all-alpha domain, STATprotein DNA-binding domain and SH2 domain of stat6, and the zinc-ﬁnger domain, leucine-zipperdomain and fork-head domain of foxp3. In addition,for stat6 and foxp3, there were ... potentiates the expression of IFN-c, which, in turn, upregulates the inducible chain of the IL-12 receptor (IL-12Rb2).Binding of IL-12 to its receptor induces signallingthrough STAT4, which further ... proteins, a leucine zipper domain and a C2H2 zinc ﬁnger domain, both of which arethought to help mediate DNA binding and may beinvolved in the induction of dimerization [43]. Each of these...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

... PAGE and subjected to autoﬂuorography. The top arrow points to SmSmad4, and the bottom arrow points to SmSmad2 in vitro translated,35S-labeled proteins. In lane 3, there isa distortion of the ... representing the 5¢- and 3¢-UTRs areshown in white in the genomic gene and the cDNA. Intron size in bp, domain size in bp and domain size in amino acids are indicated at the bottom of each schematic ... genes from other species. For example, the loca-tion of the intron within the MH1-encoding region isconserved in the human Smad4 gene, whereas the intron within the linker-encoding region of SmSmad1Bis...

Tài liệu Báo cáo khoa học: Identiﬁcation of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

... between the EAD and RNA polymer-ase II subunit [11,45]. The interaction between the EAD and RGG3 might inhibit the high-affinity Htelobinding of RGG3. To gain further insight into the induction of ... Htelo(Fig. S6). These findings indicate the importance of the guanidinium group of the Arg in RGG3 for binding to the G-quadruplex. In conclusion, EWS appears to be a DNA- and RNA-binding protein that ... domain in the C-terminal [16,17].They bind to RNA as well as single- and double-stranded DNA [18–20]. In the case of EWS, the C-terminal amino acids that constitute RGG specifi-cally bind to...

Tài liệu Báo cáo khoa học: Identiﬁcation, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx

... detection of RpCAtr mRNA in both the epidermal cytoplasm of the branchial ﬁlaments and in the cytoplasm of the trophosomal bacteriocytes. Wecould only detect RpCAbr mRNA in the epidermalcytoplasm of ... CA protein sequences. Iden-tical and similar amino acids shared by at least 50% of the isoforms are shown in black and grey, respectively. Histidine residues involved in zinc binding in the catalytic ... residues(named H94, H96 and H119 in reference to posi-tions 94, 96 and 119 in CAII from Homo sapiens)which are directly involved in binding the zinc cofac-tor, are conserved in the two R. pachyptila...

Tài liệu Báo cáo khoa học: Identiﬁcation of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx

... [4–6]. In order to improve the biological production method, adetailed understanding of the biosynthesis of triterpenesaponins is required, including the enzymes catalyzing the sequence of reactions ... reactions and the genes encoding theseenzymes. The biosynthesis of triterpene saponins involves the initial cyclization of 2,3-oxidosqualene, a common pre-cursor of all the sterol and triterpene ... range of structural diversity produced by asequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini-tial origin of structural diversity of skeletons in their biosynthesis, and...

Tài liệu Báo cáo khoa học: Identiﬁcation of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae docx

... exhibits arginineauxotrophy, but not methionine auxotrophy [2],thereby suggesting that NADPH is synthesized in the cytosol, separate from the mitochondria [2]. In this study, we identiﬁed the ... shown). The expression of POS5, UTR1 and YEF1 via ahigh-copy vector complemented the poor growth of the pos5 cell on SG solid medium and on and in solid and liquid media lacking arginine (Fig. 5 and ... 4.9aThis strain was grown in media lacking leucine. The other strainswere grown in media containing leucine.Fig. 5. Complementation of pos5 cell. Indi-cated pos5 and WT cells carrying each geneon...

Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc

... cloned into pDG148 vector to obtain the plasmid pOpac (Table 1). The respective sip(ba) geneswere isolated from initial cloning vectors and cloned into the vector pQE16 in frame with the His-tag-encodingsequence. ... described in Methods and renaturation of the SDS/PAGE gel was according to Foster [45]. Lane 1, GBA15; lane 2,GBA12. The arrows indic ate protein bands reduced or lacking in the mutant. The labels ... speci®c protein sorting [13,24,25]. Inactivation of either SipSor SipT in B. subtilis just decreased the total yields of exportproteins compared to the wild type, a s it w as also found in B. amyloliquefaciens...

Báo cáo khoa học: Identiﬁcation of the amniotic ﬂuid insulin-like growth factor binding protein-1 phosphorylation sites and propensity to proteolysis of the isoforms docx

... Cellular actions of the insulin growth factor binding proteins. Endocr Rev 23,824–854.3 Baxter RC (2000) Insulin-like growth factor (IGF-)binding proteins: interactions with IGFs and intrinsicbioactivities. ... expression of the insulin-like growthfactor-I-binding protein-1 (IGFBP-1) phosphoisoform in pre-eclamptic Wharton’s jelly and its role in the regulation of collagen biosynthesis. Clin Chem Lab ... 2009)doi:10.1111/j.1742-4658.2009.07318.xInsulin-like growth factor binding protein-1 (IGFBP-1) is the majorsecreted protein of human decidual cells during gestation and, as a modula-tor of insulin-like growth factors or by independent...

Tài liệu Báo cáo khoa học: Identiﬁcation of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc

... addition of the terminal galactose of the LOS [25], and Schwin-gel et al. found the lgt6 gene involved in the initialassembly of the LOS [26]. However, for lipid Abiosynthesis of the M. catarrhalis ... cultures of the parentalstrains and the two derived mutants from chocolate agarplates were suspended in BHI broth to a D600 nm of 0.1, and serial dilutions of these stocks were tested according to the ... hydrolysis of bacterial endotoxins and determina-tion of the anomeric conﬁguration of the glycosylphosphate present in the ‘isolated lipid A’ fragment of the Bordetella pertussis endotoxin. Carbohydr...

Tài liệu Báo cáo khoa học: Identiﬁcation of differentially expressed genes of the Paciﬁc oyster Crassostrea gigas exposed to prolonged thermal stress docx

... subjected to thermal stress are known to involve major adjustments in the expression of ribosomal genes and genes codingfor proteins involved in RNA metabolism and proteinsynthesis. In fact, protein ... assisting the folding of cytoskeletal proteins that are actively synthesized and ⁄ or renatured under these conditions. The upregu-lation of all of these chaperones conﬁrms the severity of the thermal ... against cellular stress, in ammation and stimula-tion of immune function appear to be importantcomponents of responses to thermal stress. HSPs playan essential role in maintaining protein...

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