Báo cáo khoa học: Induction of PPARb and prostacyclin (PGI2) synthesis by Raf signaling: failure of PGI2 to activate PPARb potx

... highly potent PPARb agonists, if exist-ent, remain to be identiﬁed.Results Induction of prostanoid synthesis by C -Raf signaling To investigate the effect of Raf signaling on prostanoid synthesis ... thedramatic induction of PGE2 and PGI2 synthesis after Raf activation. The strong induction of Cox-2 expres-sion was virtually abolished by both the ERK inhibitorUO126 and the RNA polymerase inhibitor ... Cox-2 above.Effect of Raf activation on the transcriptionalactivity of PPARb The simultaneous upregulation of PGI2 synthesis and PPARb expression suggested the induction of an auto-crine ⁄ intracrine...

Báo cáo khoa học: Saccharomyces cerevisiae Ybr004c and its human homologue are required for addition of the second mannose during glycosylphosphatidylinositol precursor assembly ppt

... cova-lently attached to the C-termini of certain secretoryproteins and act as anchors to attach such proteins to the outer face of the plasma membrane [1,2]. Synthesis of GPIs is essential for ... mannose(Man3) of all yeast GPIs [9] and of certain humanGPIs [10–12], and additional side-branching phospho-ethanolamines (EthN-Ps) may be added to the ﬁrst and second mannoses of yeast [13–15] and mammalianGPIs ... FEBSaddition of the third [21,22] and fourth [31] mannoses to GPI precursors (Fig. 3B, and data not shown), sug-gesting that it is a GPI intermediate that forms prior to addition of Man3 and -4 to yeast...

Báo cáo khoa học: An active triple-catalytic hybrid enzyme engineered by linking cyclo-oxygenase isoform-1 to prostacyclin synthase that can constantly biosynthesize prostacyclin, the vascular protector pot

... (AA) is ﬁrst convertedinto prostaglandin G2(PGG2, catalytic step 1), and then to prostaglandin endoperoxide [prostaglandin H2(PGH2)] (catalytic step 2) by COX isoform-1 (COX-1)or COX-2 ... downstream synthases, and is isom-erized to prostaglandin D2, prostaglandin E2(PGE2),prostaglandin F2(PGF2), and prostaglandin I2(PGI2)or thromboxane A2(TXA2) by individual synthases(catalytic ... aggregation by up to 50%(Fig. 6B, bar 2).Competitively upregulating PGI2biosynthesis inthe presence of platelets To further demonstrate the competitive upregulation of PGI2biosynthesis by COX-1–10aa–PGIS...

Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx

... suppressed by the Src-speciﬁc inhibitorCGP77675 as well as by the MEK1 inhibitor UO126 and partially attenuated by the PI3K inhibitor Wort-mannin. No effect was observed with inhibitors of p38MAPK, ... addi-tion to the loss of cell–cell adhesion, involves degrada-tion of the extracellular matrix and release of cells fromthe constraints of cell–cell and cell–matrix interaction.MMPs and urokinase-type ... yet fully understood.Expression of genes for these proteolytic enzymes canbe induced by various stimuli, including growth factors and integrin ligation, and has often been shown to beextracellular...

Báo cáo khoa học: Induction of raft-like domains by a myristoylated NAP-22 peptide and its Tyr mutant potx

... FEBS 1803 Induction of raft-like domains by a myristoylated NAP-22peptide and its Tyr mutantRaquel F. Epand1, Brian G. Sayer2 and Richard M. Epand1,21 Department of Biochemistry and Biomedical ... stream of nitrogen with constant rotation of a test tube to avoid separation of lipid components [12] and to deposit a uniform ﬁlm of lipid over the bottomthird of the tube. Last traces of solvent ... intensity of cross peaks between the aromaticprotons of the Tyr residue and the protons of the lipid indicate that in thepresence of cholesterol there is a change in the nature of the interaction of the...

Báo cáo khoa học: Induction of translationally controlled tumor protein (TCTP) by transcriptional and post-transcriptional mechanisms pot

... stimulation of TCTP synthesis by copper was mediated by the TCTP mRNA 3¢-UTR(3.2-fold) but not by the 5¢-UTR (0.5-fold). mRNA stabilization was found to mediate these effects of cobalt and nickel. ... does not differ ascompared to the control; (c) dioxin-, nickel- and cobalt-treated extracts show qualitative (appearance of new bands and suppression of control bands) and quantitative (signal ... mRNA were able to mediate a stimula-tory effect on luciferase expression under the inﬂuence of dioxin, copper, nickel and cobalt. On the otherhand, PMA and forskolin seem to act by a pure tran-scriptional...

Tài liệu Báo cáo khoa học: Hypothalamic malonyl-CoA and CPT1c in the treatment of obesity pptx

... regulatory step of glycolysis catalyzed by phosphofructokinase, which is used by glucose, butnot fructose. This rapidly activates AMPK rather thaninhibiting AMPK as glucose does. Therefore, fructose and ... entry of fatty acid into mitochon-dria. Thus, the steady-state level of malonyl-CoA inthese tissues is determined by the relative activities of ACC and malonyl-CoA decarboxylase.Role of the ... pheno-type to what was expected [34] and needs to beexplored further. Other areas of the brain have alsobeen shown to regulate feeding via AMPK [35,36]. Of interest is the extensive use of fructose...

Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx

... hydrogen-bondedvia one water molecule to the O3 atom of pantoate, and via two water molecules to the O4 atom of panto-ate. The Ne atom of His37 is hydrogen-bonded to theO4 atom of pantoate via a water molecule, ... inturn hydrogen-bonded to a network of water moleculesthat are hydrogen-bonded to the O1 atom of pantoatein site I. The O4 atom of pantoate in site II and theO1 atom of pantoate in site I are ... oxygen of Val175 is hydrogen-bonded to the O2 and O3 atoms of pantoate via a water molecule. This water moleculealso hydrogen bonds backbone nitrogen atoms of Glu150 and Lys151 to the O2 and O3 atoms...

Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx

... of endothelialcells and macrophages, as well as the differentiation and proliferation of VSMCs. Overexpression of KLF4induced expression of multiple anti-inflammatory and antithrombotic factors, ... surface and prolongation of clotting time following the induc-tion of KLF4 under inflammatory states, and implicat-ing KLF4 as a regulator of endothelial activation inresponse to proinflammatory ... [14] and that steroidogenic factor 1 binds to and activates the human SR-BI promoter in mouseadrenocortical cells [15]. Moreover, it was shown thatligand activated peroxisome proliferator activatedreceptor...

Tài liệu Báo cáo khoa học: Activation loop 3 and the 170 loop interact in the active conformation of coagulation factor VIIa pdf

... activity, inhibitor reactivity and sTFbinding of G372A-FVIIaThe very slow auto-activation of G372A-FVII, orrather the need to add factor IXa for activation to occur, was the ﬁrst sign of the relatively ... loop and with activation loop 2, by studying the effects of replacement with Ala [G372(223)A]. G372A-FVIIa, both in the free and TF-bound form, exhibited reduced cleavage of factor X (FX) and of ... concentrations of activeenzyme, and to ensure that all comparisons of thetwo forms of FVIIa were performed using the con-centrations of active enzyme. The enzymes werediluted to a concentration of 100...

Xem thêm