... 5¢-AGACAGCCGTTTTACACGCAG-3¢;P2 antisense, 5¢-CACCGAGAAATCGAAATCACC-3¢;P3sense, 5 ¢-TAGGAAGGTTGTATCGCGGAGG-3¢; and P3antisense, 5¢-CAAGGAAGGAGGACTGGGCTC-3¢ [28]. The locations of P1, P2 and ... primer pairs for p16were as follows: sense, 5¢-TTCCTGGACACGCTGGT-3¢; and antisense, 5¢-CAATCGGGGATGTCTGAG-3¢. The b-actin primer pairs were as follows: sense, 5¢-TCGTGCGTGACATTAAGGAG-3¢; and antisense, ... Texas SouthwesternMedical Center, Dallas, TX, USA).RNA extraction and real-time quantitative PCRTotal cellular RNA was extracted from the 293T cellsaccording to the Promega Total RNA Isolation...