Báo cáo khoa học: The catalysis of the SARS 3C-like protease is under extensive regulation by its extra domain doc
Báo cáo khoa học: The catalysis of the SARS 3C-like protease is under extensive regulation by its extra domain doc
... extra domain FEBS Journal 273 (2006) 1035–1045 ª 2006 The Authors Journal compilation ª 2006 FEBS 1045 The catalysis of the SARS 3C-like protease is under extensive regulation by its extra domain Jiahai ... anddeleted SARS 3C-like protease constructsThis material is available as part of the online articlefrom http://www.blackwell-synergy.comJ. Shi and J. Song SARS 3C-like protease regulation by its extra ... respiratory syndrome 3C-like protease reveals the critical role of the extra domain in dimeriza-tion of the enzyme: defining the extra domain as a newtarget for design of highly specific protease inhibitors.J...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... of the ligands,OD2 of Asp165, NE2 of His79 and NE2 of His169,formed an equatorial plane. The other protein ligand,NE2 of His31, bound to the metal, in the company of a water oxygen, from the ... ApeSOD. This implies that the conformationaround the active site of ApeSOD is independent of the presence of a metal cofactor.Figure 3D shows the superimposition of the activesite structures of apo, ... [26]. These findingslead to the hypothesis that Fe-bound ApeSOD mimics the product-inhibited form and the shift of Tyr39 sup-presses the release of the peroxide product. This maybe one of the...
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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt
... in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo assembly of the metallocofactor of C. ammoniagenes R2F is envis-aged. This is based: ... RNR and that of other class 1RNRs or, instead, is a result of the interaction of the R2F with the inactivated (oxidized) form of the R1E.We consider the first option unlikely. Under these cir-cumstances, ... from the R1 subunit back to the tyro-sine Y122 of the R2 subunit; and (d) dedocking of the product deoxyribonucleotide and reduction of the disulfide cysteine by NADPH. In in vitro stud-ies, the...
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Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf
... concomitant loss of the electron density of the loop region between b3 and b4 near the Thr-binding site are observed. The mechanism of regulation by Thr is discussedon the basis of the crystal structures. ... regulatory domain away from the kinase domain. Accompanied by the rotation of the regula-tory domain, other loops from the catalytic domainsare displaced to orient the residues important forcofactor ... intermediate [8,9], is Thr-sensitive [1]. In a2b2-type AK, the N-terminalregions of the a-subunits serves as catalytic domains,and the C-terminal regions of the a-subunits and the b-subunits act as...
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Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx
... sup-press the activation (1000-fold induction) of the IFN-bgene promoter by TRIF (Fig. 7A). This result was alsosupported by the results of the dimerization analysis of IRF-3 (Fig. 7B). Furthermore, ... determine if its activa-tion is mediated by the TLR3 but not the TLR4signaling pathway, we examined whether or not activa-tion of IRF-3 by M-pIC treatment is specifically medi-ated by the TLR3 ... of IRF-3 by M-pIC treatment is mediated by the TLR3 ⁄ TRIF signaling pathway. We obtainedfurther evidence by examining the status of the phos-phorylation and dimerization of IRF-3. The results050100150200AB691234...
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Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx
... cleavage is masked by aconformational change. The simplest interpretation of this result is that the rate of substrate cleavage is not equivalent to the rate of chemistry, and that the rate of chemistry ... dissociationFor the fraction of substrates that do undergo the reaction, the resultant products dissociate from the ribozyme relatively slowly on the time scale of the reaction. Furthermore, dissociation of ... TR (1990) Catalysis of RNAcleavage by the Tetrahymena thermophila ribozyme. 2.Kinetic description of the reaction of an RNA substratethat forms a mismatch at the active site. Biochemistry29,...
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Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx
... foundat this position (see below). The southern boundary of the active site cleft of DESC1 is formed by the 145autolysis loop. The backbone of this loop differs mark-edly from the other serine ... and thesecan be exploited by the design of specific inhibitors.To the west, the pocket is limited by Lys224 sidechain. The flexibility of this side chain is reducedCrystal structure of the ... residues.S1¢⁄S2¢ The S1¢⁄S2¢ site is located east of the active siteSer195. It is limited by the 60- (north), 37- (east) and145-loops (south). The bottom of the hydrophobicS1¢⁄S3¢ pocket is built by the...
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Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc
... indicates the C-terminal part. The dimer’s formation is established by the incorporation of the b8-strand of one monomer into a b-sheet of the other monomer. The position of the zinc ion is indicated by ... at the surface of the hexamer. Figure 4 illus-trates that the complete active site cavity is formed at the level of the hexamer by two subunits related by the three-fold axis. The main body of ... of the loop joining the b1-strand to the a1-helix, is located close to the metal ion (Fig. 3); its Od2atom is positioned in a distance of 4.2 A˚fromzinc. The backbone conformation of this...
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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc
... related to the reduction of the PQ pool.ResultsAs reported in the literature, the I step of the O–J–I–Pfluorescence transient cannot be clearly distinguished by visual analysis of the FI traces ... when the temperature is raised. The effect of DCMU on the traces was to increase the amplitude of step J with the concurrent decline of stepI, and to retard the rise to Fm. With dPQ, the J ... amplitudes and rate constants of the two other phases. The above should be gov-erned by the balance between the rate of reductionand oxidation of the acceptor side of PSII by the avail-able electron...
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Tài liệu Báo cáo khoa học: An investigation of the substrate specificity of the xyloglucanase Cel74A from Hypocrea jecorina pdf
... Theywould be indistinguishable on the chromatogramsreported by Grishutin et al., as the two octasaccharideisomers present in their product mixture also coeluted[6].Further proof of hydrolysis at substituted ... 4, and may be the basis for the unique mode of action of this enzyme.More definitive statements await the solution of the Cel74A structure, which is currently underway.Conclusions The xyloglucanase ... reported by Grishutin et al. [6],although the strong preference of Cel74A for the last of these substrates is clear in both studies. A possiblereason for the quantitative differences is the presenceof...
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