Báo cáo khoa học: Epl1, the major secreted protein of Hypocrea atroviridis on glucose, is a member of a strongly conserved protein family comprising plant defense response elicitors potx

... Journal 273 (2006) 4346–4359 ª 2006 The Authors Journal compilation ª 2006 FEBS 4359 Epl1, the major secreted protein of Hypocrea atroviridis on glucose, is a member of a strongly conserved protein family ... andother cultivation conditions was investigated, and itsphylogenetic relationship to other proteins of the cerato-platanin family was analyzed.ResultsAnalysis of the secretome of H. atroviridis ... which have been reported to be involved in plant pathogenesis and elicitation of plant defense responses. Additionally, based on the similarity of the N-terminus to that of H. atroviridis Epl1,...

Báo cáo khoa học: Long-term extracellular signal-related kinase activation following cadmium intoxication is negatively regulated by a protein kinase C-dependent pathway affecting cadmium transport ppt

... gcaagcugacccugaaguucau; PKCa #1, ccaucggauuguucuuucuucauaa;PKCa #2, gccuccauuugauggugaagaugaa; PKCd #1, ccacuacaucaagaaccaugaguuu; and PKCd #2, ccauccacaagaaaugcaucgacaa. PKCe #1, PKCe #2 and ... DC, Marais RM, Marshall CJ & ParkerPJ (1998) Activation of the mitogen-activated protein kinase ⁄ extracellular signal-regulated kinase pathwayby conventional, novel, and atypical protein ... to the amount of protein of each experimental condition.Cell toxicity quantiﬁcation The release of adenylate kinase from damaged cells wasused as a marker of cellular toxicity. Adenylate kinaseactivity...

Tài liệu Báo cáo khoa học: Seeking the determinants of the elusive functions of Sco proteins pptx

... (P174L) is associated with a fatal neonatal hepatopathy. Thispathology is caused by a loss of normal Sco1 functionrather than gain of some aberrant action of the mutant protein [69]. Structural studies ... Encephalitozoon intestinal- is, the amoebae Entamoeba dispar and Entamoebahistolytica, and the apicomplexan Cryptosporidiumparvum. The absence of Sco genes in these organisms is not unexpected, as all of ... indications for a primaryredox role The only prokaryotic organism for which a molecularlevel description of the mechanism of Cu A assembly is available is the Gram-negative thermophile T. thermo-philus....

Tài liệu Báo cáo khoa học: Resolving the native conformation ofEscherichia coli OmpA docx

... and large channels; a truncate containing just the 170 residues of the N-terminal domain gave riseonly to the narrow channels, indicating that the C-ter-minal portion takes part in formation ... modiﬁca-tion(s) of OmpA occur in the periplasm. The effect of cOHB-modiﬁcation of the C-terminaldomain in the periplasm on the transition to the large-pore conformationIn order for the large ... (A) : M-OmpA andI-OmpA at 22 °C. Lower traces (B): M-OmpA and I-OmpA at 22 °Cafter incubation at 40 °C for 2 h. The closed state is indicated by the bar at the right of each trace. The clamping...

Tài liệu Báo cáo khoa học: Understanding the binding properties of an unusual metal-binding protein ) a study of bacterial frataxin pdf

... spectrum of CyaY, but the most striking consequence of the addi-tion was the total disappearance of speciﬁc resonanceswithout the concomitant appearance of other signalsin other parts of the spectrum. ... D55,938–940.Supplementary material The following supplementary material is availableonline:Fig. S1. CyaY titration with Zn2+.Table S1. RMSD after backbone superimposition.This material is available as part of ... even at verylow ion⁄ protein ratios: at a 1 : 1 Fe2+⁄ protein ratio, the resonances of Arg20, Asp22 and Asp23 disap-peared, and the resonance of Leu21 shifted. At a 2 : 1ratio, the resonances...

Tài liệu Báo cáo khoa học: Malaria)the global disease docx

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Tài liệu Báo cáo khoa học: Probing the molecular determinants of aniline dioxygenase substrate speciﬁcity by saturation mutagenesis docx

... BL21(DE3)Activityagainst 2MAatdA1 pACYC A2 and pET A3 A 4A5 –atdA2 pACYC A1 and pET A3 A 4A5 +atdA3 pACYC A1 A2 and pET A4 A5 –Control (no deletion) pACYC A1 A2 and pET A3 A 4A5 +E. L. Ang et al. Substrate ... be a practical and valuable biocatalyst for the remediation of harmful aromatic amine contaminants.Aniline dioxygenase (AtdA) is a multicomponentenzyme isolated from Acinetobacter sp. strain ... engineering of aniline dioxygenases for the deami-nation of aromatic amines, bioremediation, and otherindustrial applications.ResultsSubstrate speciﬁcity of AtdAAs the substrate range of AtdA had not...

Tài liệu Báo cáo khoa học: Glucuronate, the precursor of vitamin C, is directly formed from UDP-glucuronate in liver pptx

... UDP-glucuronate and UDPGlcNAc, and the assay was initiated by addition of these two nucleotides.Where indicated, the assay was initiated by the addition of the enzyme preparation to an otherwise complete assaymixture. ... that the cofactorwas heat stable. This heat-stable cofactor was dependent on ATP-Mg for its action and the inhibition that it exer-ted together with ATP-Mg was antagonized by metyra-pone, aminopyrine ... glucuronidation–deglucuronidation cycle, with a hypothetical acceptorpresent in the microsomal fraction. Against this is the ﬁnding that saccharo-1,4-lactone did not affect the for-mation of...

Tài liệu Báo cáo khoa học: "Modeling the Translation of Predicate-Argument Structure for SMT" ppt

... verbal predicate, the translation of the head word of the argument, as well as the boundarywords of the translation of the argument are used asfeatures.4.3 TrainingTo train the argument reordering ... of A p the translation of A h the leftmost word of the translation of A the rightmost word of the translation of A Table 2: Features adopted in the argument reorderingmodel.Reordering CategoryPercentNC ... sentences and thus accurate trans-lations of them are desirable for SMT. We also wantto address another translation issue of arguments asshown in Table 7: arguments are wrongly translatedinto separate...

Tài liệu Báo cáo khoa học: "Learning the Latent Semantics of a Concept from its Deﬁnition" pptx

... pair similarity computation since the otherparts of speech (adjectives and adverbs) do not have a taxonomic representation structure. For example, the jcn similarity measure (Jiang and Conrath, ... Proceedings of the 50th Annual Meeting of the Association for Computational Linguistics, pages 140–144,Jeju, Republic of Korea, 8-14 July 2012.c2012 Association for Computational LinguisticsLearning ... from WN:bank#n#1: a ﬁnancial institution that accepts depositsand channels the money into lending activitiesstock#n#1: the capital raised by a corporation through the issue of shares entitling...

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