Báo cáo khoa học: Investigations into the ability of an oblique a-helical template to provide the basis for design of an antimicrobial anionic amphiphilic peptide pot
Báo cáo khoa học: Investigations into the ability of an oblique a-helical template to provide the basis for design of an antimicrobial anionic amphiphilic peptide pot
... with the anionic membranes of microbial cells, and they exert their antimicrobial action by the use of nonreceptor-based mechanisms of Keywords anionic; antimicrobial; a-helical; membrane; peptide CorrespondenceD. ... contribute to the susceptibility of E. coli model membranes to the action of AP1. An important determinant of the susceptibility of membranes to a-AMPs is the packing characteristics of the individual ... anionic a-helical peptide FEBS Journal 273 (2006) 3792–3803 ª 2006 The Authors Journal compilation ª 2006 FEBS 3803 Investigations into the ability of an oblique a-helical template to provide the basis...
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Báo cáo khoa học: Saporin and ricin A chain follow different intracellular routes to enter the cytosol of intoxicated cells pptx
... Kreitman RJ & Pastan I (1995) Importance of the glu-tamate residue of KDEL in increasing the cytotoxicity of Pseudomonas exotoxin derivatives and for increasedbinding to the KDEL receptor. ... USA).AcknowledgementsWe thank Alexandre Mezghrani, Jez Simpson, RobertoSitia and Tatiana Solda` for the critical reading of this manuscript. We are grateful to Serena Camerini(Dibit-HSR, Milan) for the amino ... at the C-terminus of PEA is essential for the cytotoxicity of the endocytosed toxin, allowingPEA to reach its site of action [2,5]. This implies thatPEA may interact with the KDEL receptor...
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Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt
... an important role in the regulation of cellular pro-cesses in plants and mammals. Moreover, they are an essential tool in the synthesis of phospholipids and phospholipid analogs. Knowledge of ... knowledge of their struc-tures cannot be generalized to plant PLDs. In this art-icle, the first information on the spatial structure of aplant a-type PLD has been obtained on the basis of recombinantly ... at the two ends of the molecule(Fig. 3). An assignment of these relaxed structures to sequence regions of PLDa2 has not yet been possible.According to the volume estimation on the basis of the partial...
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Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
... 2004significantly. The mutant E356Q had 38% and 50% of the activities for pullulan and panose, respectively. In contrast, the activity of E273Q for p ullulan was 45%, w hile that for panose remained ... isopanose from pullulan, and isomaltose andglucose from panose, and the action patterns of the wild-type and the W402F enzymes were almost identical (Fig. 2). The kinetic study for W402F towards ... W240F and W402F). The relativeactivity of mutated enzymes towards pullulan and panoseare shown in Table 2. The W 402F enzyme lost the activity for pullulan ( 0.4% of the wild-type IPU) and the...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... the existence of high and low affinity bindings of mangan-ese ions to arginase II and provide an explanation for the manganese stoichiometries determined here. Con-sidering the stoichiometry of 2 Mn2+Æsubunit)1derivedfrom ... His145Asnmutations on the affinity of metal binding to arginase II To further examine the effects of the His120fiAsnand His145fiAsn mutations on the interaction of the enzyme with manganese ions, maximally ... demonstrated for arginase[38,39] and the same may be safely deduced for agma-tinase. Therefore, as an explanation for the low cata-lytic efficiency of Asn149Asp, we conclude that the guanidino group of...
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Báo cáo khoa học: Insights into substrate and product traffic in the Drosophila melanogaster acetylcholinesterase active site gorge by enlarging a back channel ppt
... hypothesis, weused two inhibitors specific for the peripheral site thatbind to Trp321 close to the entrance: propidium andaflatoxin B1. In the wild-type DmAChE, the affinity of propidium for the ... entrance of acetylcholine to the CAS in the wild-type enzyme, it appears that, in the two mutants(Trp83Ala ⁄ Glu), inhibitors that bind to the PAS didnot prevent the entrance of substrate into ... entrance of the active site. We used 1 and 10 lm propidium and 10 and50 lm aflatoxin. The activity was followed for 1 min after the addition of acetylcholinesterase to the mixture, and the spontaneous...
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Báo cáo khoa học: Gene expression silencing with ‘specific’ small interfering RNA goes beyond specificity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx
... post-transfection,and it decreased thereafter. Thus, according to ourdata, the undesired effects of siRNA seem to appear ina transient way.Another key parameter to take into account couldbe the length of the ... is taken into account in the normalization.Furthermore, in order to consider the purity and quality of the mRNA, a second normalization step was performed on the basis of the average of fluorescence ... in the test and refer-ence samples. The presence of the internal standard probesat different locations of the array allowed quantification of the local background and evaluation of the array homoge-neity,...
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Báo cáo khóa học: Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy pptx
... direct exploitation of the structural results of the former to explain the mechanism of CaM activation of the latter. Crystallizationand resolution of the 3D structures of AC and AC–CaM,which ... 5¢-triphos-phate (Ant-dATP), than the free enzyme [22], and thereforea CaM-induced conformational change in the nucleotide-binding site was postulated. The recent resolution of the 3Dstructure of the anthrax ... led to the conclusion that the amino-acidsequence around W242, which forms an amphiphilic helicalstructure, is the ÔcoreÕ of the CaM-binding site of the enzyme[9,16–21]. The mechanism of AC...
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Báo cáo khoa học: Insight into the phosphodiesterase mechanism from combined QM ⁄ MM free energy simulations pot
... oxyanion by His234. Although the MFEP in the two-dimensional PMF suggests that an intermediate is formed and there is a barrier for the proton transfer from the intermediate, the proton trans-fer ... contributing factor is the elonga-tion of the separation of the divalent metal ions as the reaction proceeds from the Michaelis complex to the transition state, and the activation of the hydroxideion ... to span the entire range of the two reactioncoordinates from the product to the reactant states. To enhance sampling efficiency, a harmonic biasing poten-tial was applied with a force constant...
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Báo cáo khoa học: Investigations on the evolutionary conservation of PCSK9 reveal a functionally important protrusion pot
... in the binding of PCSK9 to the EGF-A domain of the LDLR, and mutations of these residues lead to loss of function, as found in our study and in the study of Kwon et al. [34]. The part of the ... and on PCSK9 cleavage by furin To study the effects of the 13 PCSK9 mutants on the PCSK9-mediated degradation of the LDLR, we usedtransiently transfected HepG2 cells and studied the amount of ... binds to the LDLR and disrupts the normal recycling of the LDLRremains to be determined. One strategy to elucidate the underlying mechanism is to study how mutationsin the PCSK9 gene affect the...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀM